Different effects of energy dependent irradiation of red and green lights on proliferation of human umbilical cord matrix-derived mesenchymal cells

Abstract

Light-emitting diodes (LED) have recently been introduced as a potential factor for proliferation of various cell types in vitro. Nowadays, stem cells are widely used in regenerative medicine. Human umbilical cord matrix-derived mesenchymal (hUCM) cells can be more easily isolated and cultured than adult mesenchymal stem cells. The aim of this study was to evaluate the effect of red and green lights produced by LED on the proliferation of hUCM cells. hUCM cells were isolated from the umbilical cord, and light irradiation was applied at radiation energies of 0.318, 0.636, 0.954, 1.59, 3.18, 6.36, 9.54, and 12.72 J/cm(2). Irradiation of the hUCM cells shows a significant (p < 0.05) increase in cell number as compared to controls after 40 h. In addition, cell proliferation on days 7, 14, and 21 in irradiated groups were significantly (p < 0.001) higher than that in the non-irradiated groups. The present study clearly demonstrates the ability of red and green lights irradiation to promote proliferation of hUCM cells in vitro. The energy applied to the cells through LED irradiation is an effective factor with paradoxical alterations. Green light inserted a much profound effect at special dosages than red light.

Effects of light emitting diode irradiation on neural differentiation of human umbilical cord-derived mesenchymal cells

“…In the current study, the effect of green and red lights on neural differentiation of hUCM cells was investigated. Some studies have reported that LED irradiation could improve cell proliferation 1 , 5 , 32 . Kim et al .…”

“…To assess differentiation capacity of the isolated cells, third passage hUCM cells at a density of 2.5 × 10 4 cells/cm 2 were seeded onto glass slides with DMEM/F12 supplemented either with adipogenic (50 μg/ml indomethacin and 100 nM dexamethasone) or osteogenic (10 nM dexamethasone, 10 mM β-glycerophosphate and 50 μg/ml ascorbic acid,) differentiation medium for 18 days. The culture media were refreshed every 72 h. Adipogenic and osteogenic differentiations were detected with Oil red O and Alizarin red staining, respectively 1 , 49 .…”

“…The power density and distribution of LED array radiation was measured by appropriate meters (Melles-Griot, US) and adjusted to 5.3 mW/cm 2 . The hUCM cells were irradiated once for five minutes (Green and RA + Green groups, separately), and one minute (Red and RA + Red groups, separately) at radiation energies of 1.59 J/cm² and 0.318 J/cm², respectively 1 (Table 1 ). The spectrum of the LED device emission was tested by the spectrometer (Avantes, The Netherlands).…”

“…In the recent years, the physical factors such as light irradiation emitted from different sources including light emitting diodes (LEDs), have been introduced as a potential physical factor for proliferation and differentiation of various cell types 1 – 3 . Peng et al .…”

Effects of maternal consumption of morphine on rat skeletal system development

“…Cell density in proliferative and hypertrophy zones of growth plate cartilage in all groups were counted in all samples. Alcian blue and alizarin red [ 31 , 32 ] were used for specific staining of cartilage and bone tissues, respectively. Comparison and evaluation of chondrogenesis and osteogenesis were performed based on a spectrophotometric assay by extracting and quantifying the amount of stain absorbed by the sections in previous experiments.…”