Different effectiveness of two pastas supplemented with either lipophilic or hydrophilic/phenolic antioxidants in affecting serum as evaluated by the novel Antioxidant/Oxidant Balance approach

Laus, Maura N.; Soccio, Mario; Alfarano, Michela; Pasqualone, Antonella; Lenucci, Marcello Salvatore; Miceli, Giuseppe Di; Pastore, Donato

doi:10.1016/j.foodchem.2016.10.050

Cited by 26 publications

(33 citation statements)

References 39 publications

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“…This has the added dimension with respect to LOX/RNO method to be a suitable tool for both in vitro measurements of food extracts and ex vivo analysis of human blood samples. In the light of this, it has been applied mainly to ex vivo AC measurements of human blood after both short-term [34] and long-term [35] intakes of food antioxidants.…”

Section: Methods Based On the Use Of The Soybean Lipoxygenase-1 Ismentioning

confidence: 99%

Assessment of Antioxidant Capacity and Putative Healthy Effects of Natural Plant Products Using Soybean Lipoxygenase-Based Methods. An Overview

et al. 2018

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In the last decades, increasing demand of antioxidant-rich foods and growing interest in their putative role in prevention of degenerative diseases have promoted development of methods for measuring Antioxidant Capacity (AC). Nevertheless, most of these assays use radicals and experimental conditions far from the physiological ones, and are able to estimate only one or a few antioxidant mechanisms. On the other hand, the novel LOX/RNO and LOX–FL methods, based on secondary reactions between the soybean lipoxygenase (LOX)-1 isoenzyme and either 4-nitroso-N,N-dimethylaniline (RNO) or fluorescein (FL), may provide a more comprehensive AC evaluation. In fact, they are able to detect simultaneously many antioxidant functions (scavenging of some physiological radical species, iron ion reducing and chelating activities, inhibition of the pro-oxidant apoenzyme) and to highlight synergism among phytochemicals. They are applied to dissect antioxidant properties of several natural plant products: food-grade antioxidants, cereal and pseudocereal grains, grain-derived products, fruits. Recently, LOX–FL has been used for ex vivo AC measurements of human blood samples after short- and long-term intakes of some of these foods, and the effectiveness in improving serum antioxidant status was evaluated using the novel Antioxidant/Oxidant Balance (AOB) parameter, calculated as an AC/Peroxide Level ratio. An overview of data is presented.

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Section: Methods Based On the Use Of The Soybean Lipoxygenase-1 Ismentioning

confidence: 99%

Assessment of Antioxidant Capacity and Putative Healthy Effects of Natural Plant Products Using Soybean Lipoxygenase-Based Methods. An Overview

et al. 2018

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“…TAC assessment is an established methodology for measuring the overall antioxidant defence system; in recent years, a variety of assays with different mechanisms have been developed to monitor TAC [7][8][9]. Very recently, a novel approach has been developed, which is based on the determination of Antioxidant/Oxidant Balance, representing the ratio between serum antioxidant capacity and serum oxidant status [10].An increasing number of studies focus on the role of ROS in the pathogenesis of numerous diseases of civilisation, such as obesity [11]. It has been suggested that higher antioxidant potential can protect the organism against undesirable ROS activity and thus reduce the incidence of disease [12].…”

Section: Total Serum Antioxidant Capacity In Healthy Normal Weight Anmentioning

confidence: 99%

“…In physiological conditions, a great part of TAC appears to be due to endogenous component activity; however, many studies report the ability of dietary intake to modulate antioxidant status after an acute consumption of antioxidant rich foods [10,29,30]. In our study, both caloric intake and nutrient intake and food habits were quite similar between normal and overweight participants; however, normal weight male participants consumed more vegetables and both, male and female participants consumed fewer meat products per day than overweight subjects and at the same time had lower TAC.…”

Section: Gendermentioning

confidence: 99%

Total Serum Antioxidant Capacity in Healthy Normal Weight and Asymptomatic Overweight Adults

Petelin

Tedeschi

Maietti

et al. 2017

Exp Clin Endocrinol Diabetes

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Obesity and overweight are major contributors to the burden of chronic disease. Both are defined as abnormal or excessive fat accumulation and by increased production of free radicals leading to oxidative stress. The aim of the present study was to evaluate whether overweight and fat accumulation is associated with serum total antioxidant capacity (TAC) in men and women, irrespective of nutritional habits, nutrient intakes, physical activity, smoking, and other confounders, which may be responsible for modifying the association between serum TAC and overweight/obesity measures. This cross-sectional study was conducted on 60 normal weight and 60 overweight adults aged 25-49. All participants underwent standard anthromorphological measurements of body composition, blood pressure and biochemical measurements, aerobic capabilities assessment and dietary intake evaluation. TAC was measured by using the photochemioluminescence method. All data were analysed with SPSS software. Men had higher values of TAC than women and concentrations of TAC were significantly higher in overweight subjects compared to normal weight subjects. In the present study TAC tended to be increased by various metabolic risk factors, especially overweight/obesity parameters (body mass index, body fat), inflammation and increased serum levels of Cysteine, irrespective of nutritional habits, nutrient intakes, physical activity and smoking. Overweight and obesity at an early stage may stimulate TAC. Therefore, the elevation of TAC in overweight adults may be a compensatory response to oxidative stress, generated by reactive oxygen species.

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“…Both parameters of the redox balance were assessed together as the ratio of total antioxidant defenses and oxidants, namely, antioxidant oxidant balance (AOB), previously applied in Ciliberti et al (21), where it was demonstrated a reliable index for the evaluation of the effects of diet on OS activated by heat stress exposition in sheep. Moreover, in human studies, it was applied in order to evaluate the antioxidant status of serum (22,23) and after long-term intake of plant antioxidant-rich foods (24). An increase in the ratio indicates a lower risk for OS exposition due to a prompter antioxidant defense against pro-oxidant production.…”

Section: Determination Of Ros Production Total Antioxidant Capacitymentioning

confidence: 99%

Nexus Between Immune Responses and Oxidative Stress: The Role of Dietary Hydrolyzed Lignin in ex vivo Bovine Peripheral Blood Mononuclear Cell Response

et al. 2020

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The control of immune responses is particularly critical when an increase of oxidative stress occurs, causing an impairment of immune cell response and a condition of systemic inflammation, named oxinflammation. Nutritional strategies based on the use in the diet of phytochemicals extracted from plants, rich in antioxidants, could help restore the antioxidant/oxidant balance and obtain a modulation of immune response. Lignin represents a valuable resource of phenolic compounds, which are characterized by a corroborated antioxidant effect. To date, there are no studies reporting the effects of lignin in the diet on immune responses and oxidative stress in ruminants. The objective of the present experiment was the evaluation of the dietary inclusion of Pinus taeda hydrolyzed lignin on the ex vivo immune responses and oxidative stress biomarkers by peripheral blood mononuclear cells (PBMCs) isolated from beef steers. In order to test the effect during oxidative stress exposition, cells were treated with hydrogen peroxide (H 2 O 2). The proliferation test and the viability assay were carried out on cells, whereas, on supernatants, the cytokine profile and the oxidative stress biomarkers were evaluated. The dietary inclusion with P. taeda hydrolyzed lignin resulted in cytoprotection after H 2 O 2 exposition, increasing the number of viable monocytes and decreasing the reactive oxygen/nitrogen species production in supernatants. The cytokine profile indicated the modulatory role of hydrolyzed lignin on immune response, with a concomitant decrease of TNF-α and increase of IL-8 production, which are strictly connected with monocyte activation and antioxidant response pathway. These results demonstrated that hydrolyzed lignin may provide a modulation of oxidative stress and inflammatory response in PBMCs; thus, the P. taeda hydrolyzed lignin could be suggested as an innovative phytochemical in ruminant feed.

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Different effectiveness of two pastas supplemented with either lipophilic or hydrophilic/phenolic antioxidants in affecting serum as evaluated by the novel Antioxidant/Oxidant Balance approach

Cited by 26 publications

References 39 publications

Assessment of Antioxidant Capacity and Putative Healthy Effects of Natural Plant Products Using Soybean Lipoxygenase-Based Methods. An Overview

Assessment of Antioxidant Capacity and Putative Healthy Effects of Natural Plant Products Using Soybean Lipoxygenase-Based Methods. An Overview

Total Serum Antioxidant Capacity in Healthy Normal Weight and Asymptomatic Overweight Adults

Nexus Between Immune Responses and Oxidative Stress: The Role of Dietary Hydrolyzed Lignin in ex vivo Bovine Peripheral Blood Mononuclear Cell Response

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