Different diversification histories in tropical and temperate lineages in the ascomycete subfamily Protoparmelioideae (Parmeliaceae)

Singh, Garima; Grande, Francesco Dal; Schnitzler, Jan; Pfenninger, Markus; Schmitt, Imke

doi:10.3897/mycokeys.36.22548

Cited by 7 publications

(7 citation statements)

References 56 publications

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“…Lichens with high selectivity may associate with a limited number of photobionts. Numerous lichen-forming fungi have been shown to associate with identical species of Trebouxia, while others exhibited higher photobiont flexibility (Kroken and Taylor 2000;Ohmura et al 2006Ohmura et al , 2018Doering and Piercey-Normore 2009;Leavitt et al 2013Leavitt et al , 2015 Photobiont diversity can be shaped by the reproductive and dispersal strategies of the mycobiont (Cao et al 2015;Steinová et al 2019), geography (Muggia et al 2014;Werth and Sork 2014;Leavitt et al 2015), growth substrate (Bačkor et al 2010;Leavitt et al 2013;Muggia et al 2014) and macroclimate (Lu et al 2018;Singh et al 2018). Lichens that reproduce sexually via independent dispersal of fungal spores, undergo a process of re-lichenisation.…”

Section: Discussionmentioning

confidence: 99%

Multidisciplinary approach to describe Trebouxia diversity within lichenized fungi Buellia zoharyi from the Canary Islands

et al. 2020

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The Canary Islands are famous for their extraordinary biodiversity; however, lichenized algae have only been studied partially. Buellia zoharyi is a circum-Mediterranean/Macaronesian species that usually occurs in semi-arid areas of the Mediterranean, but occasionally some interesting communities of this species grow on basaltic lava flows in Lanzarote, Fuerteventura and Tenerife. Those three locations showed similar ecological conditions, but different mean annual temperatures. Here we applied a multidisciplinary approach to describe microalgae diversity from B. zoharyi covering the entire described range of distribution in the Canary Islands. Photobionts were characterized in symbiosis using molecular and microscopic techniques. Different Trebouxia spp. were detected as primary photobiont in each island (Trebouxia cretacea-Fuerteventura, T. asymmetrica-Lanzarote and Trebouxia sp.`arnoldoi´-Tenerife). Coexistence of various Trebouxia spp. within a thallus were detected by using specific primers-PCR. Those three photobionts were isolated and cultured under laboratory conditions. Different phytohormone profiles were obtained in the isolated strains which suggest different internal signalling needs. In addition, we characterized the response of the isolated strains to different temperatures using chlorophyll fluorescence. T. asymmetrica did not modify their F v /fm values with respect to temperature acclimation. In contrast, Trebouxia sp.`arnoldoi'and T. cretacea were more sensitive to changes in growing temperature decreasing Fv/fm at 17°C. Our results indicate that B. zoharyi is flexible regarding the photobiont choice depending on the region, and suggest that bioclimatic factors could influence the myco/photobiont association patterns.

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Section: Discussionmentioning

confidence: 99%

Multidisciplinary approach to describe Trebouxia diversity within lichenized fungi Buellia zoharyi from the Canary Islands

et al. 2020

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“…In addition to the predominant myco-and photobionts, additional fungal and green algal/cyanobacterial species are often associated with a lichen thallus, in addition to nonphotosynthetic bacteria (Grube et al 2009;Lawrey and Diederich 2003;Muggia et al 2013). Photobiont diversity can be shaped by reproductive and dispersal strategies of the mycobiont (Cao et al 2015;Steinova et al 2019), geography (Muggia et al 2014;Werth and Sork 2014;Leavitt et al 2015), growth substrate (Bačkor et al 2010;Leavitt et al 2013b;Muggia et al 2014), and macroclimate (Lu et al 2018;Singh et al 2018). The diversity of photobionts has been only recently explored by environmental DNA metabarcoding approaches and has focused on species within the Mediterranean basin to date (Moya et al 2017;Dal Grande et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Metagenomic data reveal diverse fungal and algal communities associated with the lichen symbiosis

et al. 2020

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Lichens have traditionally been considered the symbiotic phenotype from the interactions of a single fungal partner and one or few photosynthetic partners. However, lichen symbioses have been shown to be far more complex and may include a wider range of other interacting organisms, including non-photosynthetic bacteria, accessory fungi, and algae. In this study, we analyzed metagenomic shotgun sequences in an attempt to characterize lichen mycobiomes. Specifically, we inferred the range of fungi associated within lichen thalli from five groups of lichenshorsehair lichens (mycobiont = Bryoria spp.), shadow lichens (taxa in Physciaceae), rock posies (Rhizoplaca spp.), rock tripes (Umbilicaria spp.), and green rock shields (Xanthoparmelia spp.). Metagenomic reads from the multi-copy nuclear ribosomal internal transcribed spacer region, the standard DNA barcode region for fungi, were extracted, clustered, and used to infer taxonomic assignments. Our data revealed diverse lichen-associated mycobiomes. Many of the members of the lichen-associated mycobiomes that were identified here have not previously been found in association with lichens. Furthermore, closely related mycobionts tended to have more similar mycobiomes. We found little evidence supporting the ubiquitous presence of Cystobasidiales yeasts in macrolichens, although reads representing this putative symbiotic partner were found in samples of Bryoria lichens, albeit in low abundance. Our study further highlights the ecosystem-like features of lichens, with partners and interactions far from being completely understood. Future research is needed to more fully and accurately characterize lichen mycobiomes and how these fungi interact with the major lichen components, the photo-and mycobionts.

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“…This species comprises the specimens recovered within ‘ P.isidiata E’ in ‘ Protoparmelia tropical clade’ in Singh et al (2015) and referred to as Maronina in Divakar et al (2017) and Singh et al (2018). Coalescent-based species delimitation inferred from the six-locus dataset supports these taxa as distinct lineage from the other isidiate samples collected from the geographically distant populations.…”

Section: Taxonomic Conclusionmentioning

confidence: 82%

“…This approach identifies a divergence time of ~102–112 Ma for families and 29–33 Ma for genera (Kraichak et al 2017). Based on this approach, the genus Maronina has been resurrected ( Maronina - Protoparmelia split ~70 Ma; Divakar et al 2017, Kraichak et al 2017, Singh et al 2018) and both genera, Protoparmelia and Maronina , have been placed together in the subfamily Protoparmelioideae (Parmeliaceae). Currently, the genus Protoparmelia comprises arctic, boreal, temperate and Mediterranean species, whereas the genus Maronina comprises subtropical and tropical species.…”

Section: Introductionmentioning

confidence: 99%

Neoprotoparmelia gen. nov. and Maronina (Lecanorales, Protoparmelioideae): species description and generic delimitation using DNA barcodes and phenotypical characters

Singh¹,

Ptroot²,

Ico³

et al. 2018

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Multilocus phylogenetic studies revealed a high level of cryptic diversity within the lichen-forming fungal genus Maronina (Protoparmelioideae, Parmeliaceae). Coalescent-based species delimitation suggested that most of the cryptic molecular lineages warranted recognition as separate species. Here we study the morphology and chemistry of these taxa and formally describe eight new species based on phenotypical and molecular characters. Further, we evaluate the use of ITS rDNA as a DNA barcode for identifying species in this genus. For the first time, we obtained an ITS sequence of Maroninaaustraliensis, the type species of the genus and showed that it is phylogenetically not closely related to species currently placed in Maronina or Protoparmelia. We assembled a dataset of 66 ITS sequences to assess the interspecies genetic distances amongst the twelve Maronina species using ITS as DNA barcode. We found that Maronina and Protoparmelia form a supported monophyletic group whereas M.australiensis is sister to both. We therefore propose a new genus Neoprotoparmelia to accommodate the tropical-subtropical species within Protoparmelioideae, with Neoprotoparmeliacorallifera as the type, N.amerisidiata, N.australisidiata, N.brasilisidiata, N.capensis, N.crassa, N.pauli, N.plurisporibadia and N.siamisidiata as new species and N.capitata, N.isidiata, N.multifera, N.orientalis and N.pulchra as new proposed combinations. We provide a key to Neoprotoparmelia and confirm the use of ITS for accurately identifying species in this group.

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Different diversification histories in tropical and temperate lineages in the ascomycete subfamily Protoparmelioideae (Parmeliaceae)

Cited by 7 publications

References 56 publications

Multidisciplinary approach to describe Trebouxia diversity within lichenized fungi Buellia zoharyi from the Canary Islands

Multidisciplinary approach to describe Trebouxia diversity within lichenized fungi Buellia zoharyi from the Canary Islands

Metagenomic data reveal diverse fungal and algal communities associated with the lichen symbiosis

Neoprotoparmelia gen. nov. and Maronina (Lecanorales, Protoparmelioideae): species description and generic delimitation using DNA barcodes and phenotypical characters

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