Different detection and stacking techniques in capillary electrophoresis for metabolomics

Grochocki, Wojciech; Markuszewski, Michał J.; Quirino, Joselito P.

doi:10.1039/c5ay02493e

Cited by 13 publications

(9 citation statements)

References 48 publications

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“…SPE, liquid–liquid extraction and various space‐time distributions of BGE components leading to pH‐mediated stacking, field‐amplified stacking, and transient ITP (tITP) are the most frequently used. All are clearly summarized in several reviews . An interesting idea is the implementation of a fritless SPE microcartridge.…”

Section: Development Of Methodology and Instrumentationmentioning

confidence: 99%

Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

2016

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This review focuses on the latest development of microseparation electromigration methods in capillaries and microfluidic devices coupled with MS for detection and identification of important analytes. It is a continuation of the review article on the same topic by Kleparnik (Electrophoresis 2015, 36, 159-178). A wide selection of 161 relevant articles covers the literature published from June 2014 till May 2016. New improvements in the instrumentation and methodology of MS interfaced with capillary or microfluidic versions of zone electrophoresis, isotachophoresis, and isoelectric focusing are described in detail. The most frequently implemented MS ionization methods include electrospray ionization, matrix-assisted desorption/ionization and inductively coupled plasma ionization. Although the main attention is paid to the development of instrumentation and methodology, representative examples illustrate also applications in the proteomics, glycomics, metabolomics, biomarker research, forensics, pharmacology, food analysis, and single-cell analysis. The combinations of MS with capillary versions of electrochromatography, and micellar electrokinetic chromatography are not included.

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Section: Development Of Methodology and Instrumentationmentioning

confidence: 99%

Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

2016

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“…It is necessary to mention also the problem with sensitivity in protein/peptide analysis by CE-MS. The most frequently used methods for improved sensitivity are various online or offline concentration techniques such as SPE, liquid-liquid extraction, pH-mediated stacking, field-amplified stacking, and transient ITP (tITP) (for reviews see [42][43][44][45][46][47][48]). Really popular is using dynamic pH junction methods for stacking large volume injections of proteins [46].…”

Section: Separation Tasksmentioning

confidence: 99%

Coupling of CE‐MS for protein and peptide analysis

Mikšı́k

2018

J of Separation Science

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The review is focused on the latest developments in the analysis of proteins and peptides by capillary electrophoresis techniques coupled to mass spectrometry. First, the methodology and instrumentation are overviewed. In this section, recent progress in capillary electrophoresis with mass spectrometry interfaces and capillary electrophoresis with matrix‐assisted laser desorption/ionization is mentioned, as well as separation tasks. The second part is devoted to applications—mainly bottom‐up and top‐down proteomics. It is obvious that capillary electrophoresis with mass spectrometry methods are well suited for peptide and protein analysis (proteomic research) and it is described how these techniques are complementary and not competitive with the often used liquid chromatography with mass spectrometry methods.

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“…The majority of the articles were from journals focusing on novel and improved analytical methods. For these articles, a noticeable amount targeted trace analysis facilitated by derivatisation and in some cases combined with sample stacking . A fewer number dealt with derivatisation to gain deeper insight into the composition of food stuff and biomarker determination in clinical samples.…”

Section: Introductionmentioning

confidence: 99%

Derivatisation for separation and detection in capillary electrophoresis (2015–2017)

Wuethrich

Quirino

2017

Electrophoresis

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Derivatisation is an integrated part of many analytical workflows to enable separation and detection of the analytes. In CE, derivatisation is adapted in the four modes of pre-capillary, in-line, in-capillary, and post-capillary derivatisation. In this review, we discuss the progress in derivatisation from February 2015 to May 2017 from multiple points of view including sections about the derivatisation modes, derivatisation to improve the analyte separation and analyte detection. The advancements in derivatisation procedures, novel reagents, and applications are covered. A table summarising the 46 reviewed articles with information about analyte, sample, derivatisation route, CE method and method sensitivity is provided.

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Different detection and stacking techniques in capillary electrophoresis for metabolomics

Abstract: In the last decade, capillary electrophoresis (CE) has joined other analytical separation techniques that are widely used in metabolomics.

Cited by 13 publications

References 48 publications

Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

Coupling of CE‐MS for protein and peptide analysis

Derivatisation for separation and detection in capillary electrophoresis (2015–2017)

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