2016
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Abstract: The cryopreservation of testicular tissue is presented as the only alternative for the preservation of genetic material from prepubertal animals. However, this biotechnology is still being tested. The objective of this study was to evaluate the effect of different associations of cryoprotectants and the potential of cell proliferation after vitrification of testicular tissue of prepubertal cats. Five testicular pairs from five prepubertal cats were used, and each pair was divided into four fragments. Of these,… Show more

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Cited by 13 publications
(15 citation statements)
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References 31 publications
(50 reference statements)
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“…Long-term preservation of testicular tissues provides more options to maintain genetic diversity and sustainability in populations of rare and endangered species. Despite other techniques available for pubertal animals (including the rescue of epididymal sperm cells) to subsequently transmit genes to the next generation, testicular tissue is the only biomaterial for preserving the fertility of prepubertal animals that died unexpectedly [ 1 , 2 ]. In addition, oncological treatments for young boys are gonadotoxic and may lead to infertility [ 3 ].…”
Section: Introductionmentioning
confidence: 99%
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“…Long-term preservation of testicular tissues provides more options to maintain genetic diversity and sustainability in populations of rare and endangered species. Despite other techniques available for pubertal animals (including the rescue of epididymal sperm cells) to subsequently transmit genes to the next generation, testicular tissue is the only biomaterial for preserving the fertility of prepubertal animals that died unexpectedly [ 1 , 2 ]. In addition, oncological treatments for young boys are gonadotoxic and may lead to infertility [ 3 ].…”
Section: Introductionmentioning
confidence: 99%
“…Thus, the cryopreservation of testicular tissue can be an option to preserve the fertility of these cancer patients [ 4 ]. Previous studies aiming at establishing optimal protocols to protect biological material for future utilization in assisted reproduction have been conducted in different species such as mouse [ 5 ], humans [ 6 ], cats [ 1 ], dogs [ 7 ], and wild species [ 8 ]. However, a lot of progress remains to be done in male fertility preservation.…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…Compared with the PDX model, tissue slice cultivation represented a more convenient and practical model in light of the shorter culture cycle and simpler operation. Certainly, the insert support was helpful for maintaining slice viability, and enabled the implementation of a steady platform and provided an ample supply of oxygen and nutrition, compared with floating alone (18,26,27). On the whole, precision-cut slice cultivation retained the heterogeneous architecture of the original tumor and thus might provide a basis for in-depth study of pharmacokinetic mechanisms in pharmacology and gene pathways in tumor biology.…”
Section: Discussionmentioning
confidence: 99%
“…After the testicular tissue is exposed to the vitrification solution, fragments are placed in a metal cube held above liquid nitrogen in an insulated box, which enables the tissue to be cooled ultrarapidly. Upon cooling, these fragments are placed in cryotubes and kept in liquid nitrogen (LIMA et al, 2016). Among all the testicular cryopreservation techniques, vitrification is the one with the lowest operating costs and is the easiest to carry out.…”
Section: Cryopreservation Methods Of Testicular Tissuementioning
confidence: 99%