Differences on the microsporogenesis and tapetal development of male fertile and cytoplasmic male sterile pepper (<i>Capsicum annuum</i> L.)

Qiu, Yi; Liao, Lijuan; Liu, Shengzi; Mao, Dandan; Liu, Ru‐Shi

doi:10.1080/00173134.2016.1248860

Cited by 10 publications

(2 citation statements)

References 32 publications

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“…In this sense, the counting at anaphase I, prophase II, metaphase II, anaphase II, and telophase II revealed PMCs with plates and nuclei with an unbalanced number of chromosomes (Table 4). Finally, cytomixis was observed in all the divisional stages of PMCs, a fact that would contribute to the abnormal meiotic behavior, since the transfer of different amounts of chromatin results in unbalanced complements of the PMCs involved (Guan et al., 2012; Qiu, Liao, Liu, Mao, & Liu, 2017). Therefore, all the abovementioned meiotic abnormalities would lead to the formation of microcytes, as well as of sporads with more than four microspores (pentads and hexads) or sporads with four microspores with different sizes (Table 3).…”

Section: Resultsmentioning

confidence: 99%

Occurrence of 2n microspore production in diploid interspecific hybrids between the wild parental species of peanut (Arachis hypogaea L., Leguminosae) and its relevance in the genetic origin of the cultigen

et al. 2020

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Unreduced (2n) gametes are the driving force that leads to polyploidization of plants in nature and an important tool for ploidy breeding. Peanut (Arachis hypogaea L., Leguminosae) seems most likely to have originated by sexual polyploidization through interspecific hybridization between the wild diploid relatives—A. duranensis Krapov. & W.C. Greg. and A. ipaënsis Krapov. & W.C. Greg—and subsequent binding of the unreduced gametes in the hybrid. Therefore, aiming to contribute to understanding the event of polyploid origin of this important crop, we made diploid hybrids between A. duranensis and A. ipaënsis and then investigated the production of unreduced gametes in the hybrids by analyzing the size range of pollen, the constitution of the sporads, and the microsporogenesis process. The meiotic abnormalities found, such as persistent bridges, tripolar spindles, and cytomixis processes, would lead to the formation of restitution nuclei in both the first and second meiotic division. These failures in the regularity of meiosis lead to the formation of dyads and triads, and consequently to 2n pollen grains. These findings constitute the first evidence of the formation of unreduced gametes in intergenomic hybrids obtained between the wild progenitors of A. hypogaea and enable us to propose a model of origin of the crop through sexual polyploidization.

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Section: Resultsmentioning

confidence: 99%

Occurrence of 2n microspore production in diploid interspecific hybrids between the wild parental species of peanut (Arachis hypogaea L., Leguminosae) and its relevance in the genetic origin of the cultigen

et al. 2020

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“…Previous studies have demonstrated that programmed cell death and ROS stress occur in the tapetum to affect the provision of nutrients and metabolites for microspore development in male sterility ( Ariizumi & Toriyama, 2007 ; Guo et al, 2017 ). The tapetal layer is essential for pollen grain development because it is the site of synthesis for proteins, which are then transported to the anther walls ( Qiu et al, 2016 ; Yang et al, 2014 ). Male sterility in Arabidopsis thaliana is associated with microspore development due to premature or delayed tapetal programmed cell death ( Ariizumi & Toriyama, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of differential anther development from sterile/fertile lines in Capsicum annuum L.

Pei

Xie

Wang

et al. 2022

PeerJ

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Background Pepper (Capsicum annuum L.) is a major cash crop throughout the world. Male sterility is an important characteristic in crop species that leads to a failure to produce functional pollen, and it has crucial roles in agricultural breeding and the utilization of heterosis. Objectives In this study, we identified many crucial factors and important components in metabolic pathways in anther and pollen development, and elucidated the molecular mechanism related to pollen abortion in pepper. Methods Pepper pollen was observed at different stages to detect the characteristics associated with male sterility and fertility. The phytohormone and oxidoreductase activities were detected in spectrophotometric and redox reaction assays, respectively. Proteins were extracted from male sterile and fertile pepper lines, and identified by TMT/iTRAQ (tandem mass tags/isobaric tags for relative and absolute quantitation) and LC-MS/MS (liquid chromatograph-mass spectrometer) analysis. Differentially abundant proteins (DAPs) were analyzed based on Gene Ontology annotations and the Kyoto Encyclopedia of Genes and Genomes database according to |fold change)| > 1.3 and P value < 0.05. DAPs were quantified in the meiosis, tetrad, and binucleate stages by parallel reaction monitoring (PRM). Results In this study, we screened and identified one male sterile pepper line with abnormal cytological characteristics in terms of pollen development. The peroxidase and catalase enzyme activities were significantly reduced and increased, respectively, in the male sterile line compared with the male fertile line. Phytohormone analysis demonstrated that the gibberellin, jasmonic acid, and auxin contents changed by different extents in the male sterile pepper line. Proteome analysis screened 1,645 DAPs in six clusters, which were mainly associated with the chloroplast and cytoplasm based on their similar expression levels. According to proteome analysis, 45 DAPs were quantitatively identified in the meiosis, tetrad, and binucleate stages by PRM, which were related to monoterpenoid biosynthesis, and starch and sucrose metabolism pathways. Conclusions We screened 1,645 DAPs by proteomic analysis and 45 DAPs were related to anther and pollen development in a male sterile pepper line. In addition, the activities of peroxidase and catalase as well as the abundances of phytohormones such as gibberellin, jasmonic acid, and auxin were related to male sterility. The results obtained in this study provide insights into the molecular mechanism responsible for male sterility and fertility in pepper.

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Review on tapetal ultrastructure in angiosperms

Gotelli

Lattar

Zini

et al. 2023

Planta

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Differences on the microsporogenesis and tapetal development of male fertile and cytoplasmic male sterile pepper (Capsicum annuum L.)

Cited by 10 publications

References 32 publications

Occurrence of 2n microspore production in diploid interspecific hybrids between the wild parental species of peanut (Arachis hypogaea L., Leguminosae) and its relevance in the genetic origin of the cultigen

Occurrence of 2n microspore production in diploid interspecific hybrids between the wild parental species of peanut (Arachis hypogaea L., Leguminosae) and its relevance in the genetic origin of the cultigen

Proteomic analysis of differential anther development from sterile/fertile lines in Capsicum annuum L.

Review on tapetal ultrastructure in angiosperms

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