Differences of RNA Expression in the Tendon According to Anatomic Outcomes in Rotator Cuff Repair

Ahn, Jin Ok; Chung, Jin Young; Kim, Do Hoon; Im, Wooseok; Kim, Sae Hoon

doi:10.1177/0363546517713198

Cited by 12 publications

(23 citation statements)

References 46 publications

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“…Of the studies, 2 were deemed poor quality, 79,81 15 were moderate quality, 2,4,13,27,37,39,47,49,55,56,59,64,69,70,82 and 9 were high quality. 3,7,11,12,16,38,42,47,55,70,72,80,82 These results are outlined in Table II. A further breakdown of these scores is shown in Supplementary Tables S7-S9.…”

Section: Quality Assessmentmentioning

confidence: 76%

“…7,12,37,47,59,69,79,81 13 investigated the association of altered gene expression in human shoulder tissue with RCTs, 2,4,11,13,16,39,42,49,55,56,64,70,82 and 5 investigated genetic associations with healing after surgical repair of RCTs at !1 year following surgery. 3,27,38,72,80 Study characteristics are described in Supplementary Tables S4-S6.…”

Section: Individual Study Characteristics and Resultsmentioning

confidence: 99%

“…Owing to the heterogeneity of study designs and data synthesis across studies, a qualitative analysis was applied. Gene expression findings associated with RCTs across studies were first stratified into the molecular pathway in which they have the greatest influence within the shoulder, per Ahn et al 3 ; these included extracellular matrix (ECM)-related genes, apoptotic signaling genes, immune and inflammatory response genes, and growth factor genes. Genetic findings across studies that were associated with failed healing after surgical repair of RCTs were stratified into these same molecular pathways.…”

Section: Data Synthesismentioning

confidence: 99%

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Polymorphisms and alterations in gene expression associated with rotator cuff tear and healing following surgical repair: a systematic review

Mousley

Hill-Buxton

Gill

et al. 2021

Journal of Shoulder and Elbow Surgery

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Background: Rotator cuff tears (RCTs) are a common cause of shoulder disability, yet both conservative and surgical treatment strategies can lead to poor results in some patient populations. Enhanced understanding of the genetic processes associated with RCTs can assist in the development of more effective management options and help predict individual responses to surgical treatment. This systematic review analyzes the current literature on the genetic footprint associated with RCTs and interprets these findings to enhance the current understanding of RCT pathogenesis, potential treatment regimens, and prognostic biomarkers of outcomes after surgical repair. Methods: A systematic search of the Embase, PubMed, and Web of Science electronic databases was performed. Medical Subject Headings (MeSH) and Emtree index terms were formulated from the concept terms ''rotator cuff tear,'' ''genetics,'' and ''human,'' and synonyms of these concepts were applied to the Web of Science search. Articles were screened against predefined inclusion and exclusion criteria. Eligible studies compared gene expression patterns and genetic polymorphisms between cases (with RCTs) and controls (without RCTs). Quality assessment was performed with studies being rated as high, moderate, or poor quality. A modified bestevidence synthesis was applied, and studies were determined to be of strong, moderate, or limited evidence. Results: The search identified 259 articles. Of these studies, 26 were eligible for review. Two studies were considered poor quality; 15 studies, moderate quality; and 9 studies, high quality. Analysis of these articles found that RCTs were associated with alterations in genes that code for the extracellular matrix, cell apoptosis, immune and inflammatory responses, and growth factor pathways. In particular, there was strong evidence of a significant association between RCTs and the genes MMP3, TNC, and ESRRB. Strong evidence of an association between BMP5 upregulation and successful healing after surgical repair was also found. Conclusion: This review provides strong evidence of an genetic association with RCTs. The genotype and gene expression patterns detailed within this review can assist in deciphering the biological mechanisms resulting in RCTs, as well as predicting an individual's Institutional review board approval was not required for this systematic review.

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Section: Quality Assessmentmentioning

confidence: 76%

Section: Individual Study Characteristics and Resultsmentioning

confidence: 99%

Section: Data Synthesismentioning

confidence: 99%

See 1 more Smart Citation

Polymorphisms and alterations in gene expression associated with rotator cuff tear and healing following surgical repair: a systematic review

Mousley

Hill-Buxton

Gill

et al. 2021

Journal of Shoulder and Elbow Surgery

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“…These authors found 15 SNPs of the TNC gene and they were significantly associated with degenerative lesions. 30 Ahn et al 31 evaluated 14 patients who underwent repair and concluded that negative regulation of inflammatory response genes and positive regulation of cell differentiation genes at the time of surgery are related to rotator cuff healing.…”

Section: Rotator Cuff Injurymentioning

confidence: 99%

“…Ahn et al 31 avaliaram 14 pacientes que foram submetidos ao reparo e concluíram que a regulação negativa de genes de resposta inflamatória e a regulação positiva de genes de diferenciação celular no momento da cirurgia estão relacionados à cicatrização do manguito rotador.…”

Section: Lesão Do Manguito Rotadorunclassified

Aspectos genéticos nas afecções do ombro

Cohen

Figueiredo

Belangero

et al. 2020

Rev Bras Ortop (Sao Paulo)

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ResumoA influência da herança genética tem sido cada vez mais investigada nas afecções do ombro, como a lesão do manguito rotador, instabilidade e ombro congelado. Ainda que os achados iniciais sejam pouco esclarecedores, é necessário construir progressivamente um banco de marcadores genéticos para catalogar perfis genômicos que, mais adiante, poderão contribuir para a previsão do risco da doença, desenvolvimento de melhores ferramentas de diagnóstico e tratamento. O presente artigo busca atualizar o que há de evidências de estudos genéticos na literatura para essas doenças, desde análises de polimorfismos, expressão de genes candidatos em tecidos e estudos de associação genômica ampla (GWAS, na sigla em inglês). Porém, é necessário apontar que existe grande dificuldade na replicação e utilização dos achados, principalmente em razão da falta de poder estatístico, da alta taxa de resultados falso-positivos e da grande quantidade de variáveis envolvidas.

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RNA‐Seq analysis reveals sex‐dependent transcriptomic profiles of human subacromial bursa stratified by tear etiology

Farooq

Cai

Tycksen³

et al. 2022

Journal Orthopaedic Research

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Rotator cuff tendinopathy, a major cause of shoulder disability, occurs due to trauma or degeneration. Our molecular understanding of traumatic and degenerative tears remains elusive. Here, we probed transcript level differences between traumatic and degenerative tears. Subacromial bursa tissues were collected from patients with traumatic or degenerative tears during arthroscopy (N = 32). Transcripts differentially expressed by tear etiology were detected by RNA‐seq. RNA‐seq results were validated by real‐time quantitative polymerase chain reaction. We identified 334 protein‐coding transcripts differentially expressed between traumatic and degenerative tears in females and 167 in males at a fold‐change greater than 2. In females, XIRP2, MYL1, MYBPC1, TNNT1, and LMOD2, were highly expressed in traumatic tears whereas TPSD1, CDSN, RCVRN, LTBP4, and PTGS1 were elevated in degen tears. Transcripts elevated in traumatic tears represented muscle cell differentiation and development, and muscle contraction whereas those elevated in degenerative tears represented cell activation and immune response. In males, AZGP1, CNTFR, COL9A1, ZNF98, and EREG were highly elevated in traumatic tears whereas MYL2, HOXD11, SLC6A7, CADM1, and MMP17 were highly expressed in degenerative tears. Transcripts elevated in traumatic tears represented metabolic/catabolic processes, and transmembrane protein transport while processes related to cell cycle were mainly enriched in degenerative tears. Numerous long noncoding RNAs were differentially expressed between traumatic and degenerative tears in both sexes. In summary, this study provides insights into molecular biology of bursa in patients with rotator cuff tendon disease based on tear acuity and novel sex‐based transcript differences that could inform clinical decision making in treating patients with traumatic or degenerative shoulder injuries.

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Differences of RNA Expression in the Tendon According to Anatomic Outcomes in Rotator Cuff Repair

Abstract: The downregulation of inflammatory response genes and the upregulation of cell differentiation genes in torn rotator cuffs at the time of surgery are related to rotator cuff healing. These results provide useful baseline information for future biological studies on rotator cuff healing.

Cited by 12 publications

References 46 publications

Polymorphisms and alterations in gene expression associated with rotator cuff tear and healing following surgical repair: a systematic review

Polymorphisms and alterations in gene expression associated with rotator cuff tear and healing following surgical repair: a systematic review

Aspectos genéticos nas afecções do ombro

RNA‐Seq analysis reveals sex‐dependent transcriptomic profiles of human subacromial bursa stratified by tear etiology

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