Differences of expression of cytoskeletal proteins in cultured rat hepatocytes and hepatoma cells

Franke, Werner W.; Mayer, Doris; Schmid, Erika Nardon; Denk, Helmut; Borenfreund, Ellen

doi:10.1016/0014-4827(81)90435-3

Cited by 166 publications

(73 citation statements)

References 41 publications

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“…1.). These two major polypeptides are consistent with the component A and D cytokeratins, respectively, reported by Franke et al (7,8). The bile duct-ligated rats exhibited many changes as compared to control rats.…”

Section: Resultssupporting

confidence: 88%

“…Investigation of the relationship between hepatocyte cytoskeleton and liver diseases is still at an embryonic stage and only a few reports on how the cytoskeleton of hepatocytes changes quantitatively and qualitatively in obstructive jaundice have been published (7,8,18,24). Okanoue et al (18) observed the cytoskeleton of hepatocytes from bile duct-ligated rats under both transmission and scanning electron microscopes and reported a marked increase in the intermediate filaments of hepatocytes.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cytoskeletal protein changes in hepatocytes of bile duct-ligated rats.

Hoshino

Katagiri

Takeshima

et al. 1989

Acta Histochem. Cytochem

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Section: Resultssupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Cytoskeletal protein changes in hepatocytes of bile duct-ligated rats.

Hoshino

Katagiri

Takeshima

et al. 1989

Acta Histochem. Cytochem

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“…For this, confluent HeLa cells were extracted with Triton X-100 and high salt buffers, and cytoskeletal proteins enriched in IFs were obtained as described (Franke et al, 1978;Franke et al, 1981). In the fractions, we could detect K8/18, but not trichoplein (data not shown).…”

Section: Interaction Of Trichoplein With Keratin Filaments In Vivomentioning

confidence: 94%

Identification of trichoplein, a novel keratin filament-binding protein

Nishizawa

Izawa

Inoko

et al. 2005

Journal of Cell Science

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Keratins 8 and 18 (K8/18) are major components of the intermediate filaments (IFs) of simple epithelia. We report here the identification of a novel protein termed trichoplein. This protein shows a low degree of sequence similarity to trichohyalin, plectin and myosin heavy chain, and is a K8/18-binding protein. Among interactions between trichoplein and various IF proteins that we tested using two-hybrid methods, trichoplein interacted significantly with K16 and K18, and to some extent with K5, K6a, K8 and K14. In in vitro co-sedimentation assays, trichoplein directly binds to K8/18, but not with vimentin, desmin, actin filaments or microtubules. An antibody raised against trichoplein specifically recognized a polypeptide with a relative molecular mass of 61 kDa in cell lysates. Trichoplein was immunoprecipitated using this antibody in a complex with K8/18 and immunostaining revealed that trichoplein colocalized with K8/18 filaments in HeLa cells. In polarized Caco-2 cells, trichoplein colocalized not only with K8/18 filaments in the apical region but also with desmoplakin, a constituent of desmosomes. In the absorptive cells of the small intestine, trichoplein colocalized with K8/18 filaments at the apical cortical region, and was also concentrated at desmosomes. Taken together, these results suggest that trichoplein is a keratin-binding protein that may be involved in the organization of the apical network of keratin filaments and desmosomes in simple epithelial cells.

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“…To distinguish between meningeal cells and the closely apposed fibroblasts, we wanted to identify a specific marker for meningeal cells. Unlike that of the fibroblasts (16,32), the embryonic origin of the meninges can be partly traced back to neural ectoderm-derived cells (17,20,29,38), which are known to express cytokeratins (15); this was the rationale for using an antibody against these markers to exclusively immunostain ONP meningeal cells. Immunofluorescence for PrP Sc and cytokeratins on serial tissue sections demonstrated that cytokeratins and PrP Sc colocalized inside the meningeal layer of the ONP (Fig.…”

Section: Using Immunofluorescence For Prpmentioning

confidence: 99%

Olfactory System Involvement in Natural Scrapie Disease

Corona

Porcario

Martucci

et al. 2009

J Virol

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The olfactory system (OS) is involved in many infectious and neurodegenerative diseases, both human and animal, and it has recently been investigated in regard to transmissible spongiform encephalopathies. Previous assessments of nasal mucosa infection by prions following intracerebral challenge suggested a potential centrifugal spread along the olfactory nerve fibers of the pathological prion protein (PrP Sc ). Whether the nasal cavity may be a route for centripetal prion infection to the brain has also been experimentally studied. With the present study, we wanted to determine whether prion deposition in the OS occurs also under

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Differences of expression of cytoskeletal proteins in cultured rat hepatocytes and hepatoma cells

Cited by 166 publications

References 41 publications

Cytoskeletal protein changes in hepatocytes of bile duct-ligated rats.

Cytoskeletal protein changes in hepatocytes of bile duct-ligated rats.

Identification of trichoplein, a novel keratin filament-binding protein

Olfactory System Involvement in Natural Scrapie Disease

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