Cytoskeletal protein changes in hepatocytes of bile duct-ligated rats.

Hoshino, Makoto; Katagiri, Kenji; Takeshima, Akihiko; Nakai, Tomio; Ohnishi, Hayato; Hayakawa, Tomihiro; Miyaji, Makoto; Takeuchi, Toshihiko

doi:10.1267/ahc.22.341

Cited by 3 publications

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar increase in microfilaments has been reported in ductular cells of cholestatic livers Adler et al, 1980), while no significant changes to intermediate filaments have been reported following bile duct ligation in the rat (Okanoue et al, 1988). Since intermediate filaments in hepatocytes can be increased by changes in the environment over an extended period of time in obstructive jaundice (Okanoue et al, 1988) and following experimental bile duct ligation (Hoshino et al, 1989), and the lampreys used in this study have been infected for several years, it seems plausible that the numerous intermediate filaments in bile duct cells ofL. lamottenii are induced by the resultant increased biliary pressure.…”

Section: Periductal Connective Tissuesupporting

confidence: 56%

Morphology of the bile ducts of the brook lamprey, Lampetra lamottenii (Le Sueur) before and during infection with the nematode, Truttaedacnitis stelmioides (Vessichelli, 1910) (Nematoda: Cucullanidae)

Eng

Youson

1992

Anat. Rec.

View full text Add to dashboard Cite

Routine light microscopy and transmission and scanning electron microscopy were used to describe and compare the biliary tree of larval Lampetra lamottenii before and during infestation of the bile ducts with the nematode, Truttaedacnitis stelmioides. The most prominent changes to the biliary tree following infection by the parasite are the dilation of the bile ducts, alterations to their epithelial cells, and an increase in periductal fibrous tissue. In recently infected animals, the simple epithelium of dilated bile ducts often contains many mitotic figures. In long-term infestations, the epithelium is stratified or pseudostratified. Changes to the fine structure of the biliary epithelial cells include increase and/or dilation of the RER and SER, and increases in microfilaments, intermediate filaments, and microtubules. The abundance of dense bodies may reflect enhance reabsorption of biliary constituents, and their accumulation may ultimately result in cytolysis. There are increased mucous granules in the apical cytoplasm of biliary epithelial cells and an abundance of mucinous material within the bile duct lumen, and the basal lamina appears thickened. The changes to the liver of L. lamottenii following infection are discussed and compared to those reported in small mammals following bile duct ligation, in patients with extrahepatic biliary obstruction, and in parasitic infection of the biliary tree.

show abstract

Section: Periductal Connective Tissuesupporting

confidence: 56%

Morphology of the bile ducts of the brook lamprey, Lampetra lamottenii (Le Sueur) before and during infection with the nematode, Truttaedacnitis stelmioides (Vessichelli, 1910) (Nematoda: Cucullanidae)

Eng

Youson

1992

Anat. Rec.

View full text Add to dashboard Cite

show abstract

Bile ductule formation in fetal, neonatal, and infant livers compared with extrahepatic biliary atresia

et al. 1996

View full text Add to dashboard Cite

antigen, carcinoembryonic antigen, and epithelial membrane The cell of origin of intrahepatic bile ducts during fetal antigen. [13][14][15][16][17][18][19][20][21][22][23] These studies have further supported the hepadevelopment remains a subject of controversy, although tocytogenic theory. there has been recent evidence that they form from heCytokeratins are the intermediate filaments of the cypatocytes. However, the origin of neoductules and ducts toskeleton characteristic of epithelial cells. [24][25][26][27] At least 20 in the setting of liver disease has not been extensively CKs have been distinguished on the basis of different molecuinvestigated in humans. Using anticytokeratins characlar weights and isoelectric points on two-dimensional gels. 24-26 teristic of hepatocytes and bile ducts, we repeated ear-The human embryonic liver is composed of liver cell precurlier studies of fetal development to compare ductule forsors (hepatoblasts) in its earliest developmental stages. mation in normal developing and newborn livers withThese hepatoblasts express cytokeratins 8, 18, and 19. 19 At the ductules formed during extrahepatic biliary atresia. We utilized an antibody to proliferating cell nuclear an-approximately the eighth week of gestation, the hepatoblasts tigen (PCNA) staining to determine which cells were in that are adjacent to the mesenchyme surrounding the largest active DNA synthesis (S phase) during fetal development hilar portal veins become more immunoreactive for cytokeraand liver disease progression. The results indicated that tins 8, 18, and 19. These cells, which surround the portal hepatocytes undergo a phenotypic switch (metaplasia) vein branches, become smaller and later flatten to form the to form ductular cells during fetal development. There ductal plate. 1 They become more immunoreactive to the CK was no ductular cell replication in the fetal livers. In antibodies, while the hepatoblasts away from the mesencontrast, both bile ductular metaplasia and proliferation chyme start to lose their antigenicity for cytokeratin 19, and were observed in biliary atresia. Therefore, both a lim-by 10 weeks of gestation, the future parenchymal cells are iting plate phenotypic switch to ductules and replication antigenic for only cytokeratins 8 and 18, as seen in adult of ductular cells play a role in the increase in the duct-liver parenchymal cells. ules seen in the progression to biliary cirrhosis. Bile duc-The ductal plate later becomes a double cell layer, and a tular proliferation in biliary atresia, however, was less slitlike opening begins to form between the cell layers to form than that seen in hepatocytes, whereas the number of lumens. The formation of these ductal plates starts at the bile ductules increased and the relative proportion of hilum where the largest portal vein branches are located, hepatocytes diminished as the accompanying periductu-and then progresses to the smaller portal tracts away from lar fibrosis progressed to cirrhosis. (HEPATOLOGY 1996; the hilum. At 20 weeks of gestati...

show abstract

Three-dimensional studies of the cytoskeleton of cultured hepatocytes: A quick-freezing and deep-etching study

Ohno

Fujii²

1991

Vichows Archiv A Pathol Anat

View full text Add to dashboard Cite

The ultrastructure of the cytoskeleton of cultured mouse hepatocytes was studied by a quick-freezing and deep-etching method. Isolated mouse hepatocytes were cultured on collagen gels for 48 h, fixed in paraformaldehyde and centrifuged to prepare cell pellets. The hepatocytes were split open to remove cytoplasmic soluble proteins for replica preparations. Some specimens were decorated with anti-actin antibody or S1 myosin fragments to identify actin filaments. They were quickly frozen in isopentane-propane mixture, fractured in liquid nitrogen, deeply etched in a freeze-fracture machine and rotary shadowed by platinum and carbon. The basal cell membranes of hepatocytes were in contact with the collagen gels and the apical surface faced the culture medium. Networks of actin filaments were attached to the apical cell membranes, but intermediate filaments were localized along the basal layer. Some intermediate filaments were associated with cell organelles, such as the endoplasmic reticulum. The Golgi apparatus was less associated with the cytoskeleton and showed synthesized materials in the cisternae. Cytoskeletal organization in cultured hepatocytes was revealed three-dimensionally, indicating that the interaction of cell membranes with collagen gels is important for the organization of the cytoskeleton.

show abstract

Cytoskeletal protein changes in hepatocytes of bile duct-ligated rats.

Cited by 3 publications

References 21 publications

Morphology of the bile ducts of the brook lamprey, Lampetra lamottenii (Le Sueur) before and during infection with the nematode, Truttaedacnitis stelmioides (Vessichelli, 1910) (Nematoda: Cucullanidae)

Morphology of the bile ducts of the brook lamprey, Lampetra lamottenii (Le Sueur) before and during infection with the nematode, Truttaedacnitis stelmioides (Vessichelli, 1910) (Nematoda: Cucullanidae)

Bile ductule formation in fetal, neonatal, and infant livers compared with extrahepatic biliary atresia

Three-dimensional studies of the cytoskeleton of cultured hepatocytes: A quick-freezing and deep-etching study

Contact Info

Product

Resources

About