1982
DOI: 10.1093/nar/10.12.3627
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Differences in the nuclease sensitivity between the two alleles of the immunoglobulin kappa light chain genes in mouse liver and myeloma nuclei

Abstract: In mouse myeloma T the productive kappa light chain gene differs from its aberrantly rearranged allele in the patterns of DNAase I hypersensitive sites. In the region of the alleles where they are identical in sequence they have one site in common which lies 0.8 kb downstream of the coding region; but two sites upstream of and within the C gene segment (2) are found only on the non-productive allele. Within the region of different sequences both alleles have analogously located DNAase I hypersensitive sites; t… Show more

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citations
Cited by 46 publications
(19 citation statements)
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References 32 publications
(44 reference statements)
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“…In view of the complete divergence between the enhancers of the related viruses SV40 and polyomavirus this is not surprising. The activating region also includes a site that is hypersensitive to DNase I in the chromatin of transcriptionally active K genes (3,18,29,35), a finding which is consistent with findings for various enhancers (9,24). Three short segments in the K-activating region are homologous to sequences in various enhancers.…”
supporting
confidence: 82%
“…In view of the complete divergence between the enhancers of the related viruses SV40 and polyomavirus this is not surprising. The activating region also includes a site that is hypersensitive to DNase I in the chromatin of transcriptionally active K genes (3,18,29,35), a finding which is consistent with findings for various enhancers (9,24). Three short segments in the K-activating region are homologous to sequences in various enhancers.…”
supporting
confidence: 82%
“…3, the inverse sequence (A-T-G-C-A-A-A-T), with only occasional alterations, is present 150 ± 10 bp upstream from the first RNA splice junction in all of the heavy chain genes for which adequate data are available (28)(29)(30) This observation implies that factors other than accurate joining of the V and J loci are required to activate K light chain gene transcription. Recently, attention has focused upon events occurring within a small (<250 bp) region of DNA closely linked to the CK locus (9,15,(31)(32)(33). This region, which lies -3.5 kb downstream from the site of transcriptional initiation, undergoes localized changes in chromatin structure that correlate with transcriptional activity of the gene; in 70Z/3 cells, these chromatin changes occur after exposure to LPS (9).…”
Section: Resultsmentioning
confidence: 99%
“…In fact, by analogy to immunoglobulin and T-cell receptor genes (2,38), such hypersensitivity may be indicative of an enhancer element (12). The high degree of base sequence conservation in the region of DH site 4 specific signals for differentiation (15,37).…”
Section: Discussionmentioning
confidence: 99%