Differences in the expression profile of endo-β-(1,6)- d -galactanase in pathogenic and non-pathogenic races of Colletotrichum lindemuthianum grown in the presence of arabinogalactan, xylan or Phaseolus vulgaris cell walls

Villa-Rivera, Maria Guadalupe; Zavala-Páramo, María Guadalupe; Conejo-Saucedo, Ulises; López-Romero, Everardo; Lara-Márquez, Alicia; Cano-Camacho, Horacio

doi:10.1016/j.pmpp.2016.10.002

Cited by 2 publications

(8 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are no crystallized structures of endo1,6GAL and 1,6GAL; however, a prediction of the three-dimensional structure of Colletotrichum lindemuthianum endo1,6GAL has been reported. The endo1,6GAL model adopts a (β/α)8 TIM barrel fold topology (eight-stranded parallel β-strand, forming a central barrel surrounded by eight α-helices) with a putative active site located at the C-terminus, which is consistent with the characteristic structure of GH30 family enzymes ( Figure 2C ; Villa-Rivera et al, 2017b ). These enzymes have been characterized from fungi and bacteria and have also been expressed in heterologous models ( Table 1 ).…”

Section: Degradation Of Ags By Enzymes Of Microorganismssupporting

confidence: 68%

“…Deletion of the MoAbfB gene encoding an α -N- arabinofuranosidase B from Magnaporthe oryzae resulted in a reduction in disease severity in rice ( Wu et al, 2016 ). Likewise, comparison of the genetic expression of an endo1,6GAL between pathogenic and non-pathogenic strains of C. lindemuthianum , showed that compared with most of the evaluated conditions, the levels of genetic expression were higher in pathogenic than non-pathogenic strains, supporting a role for this enzyme in the PCW degradation during the establishment of the infection ( Villa-Rivera et al, 2017b ). Finally, the inactivation of Malus domestica AGPs with β-Glc Yariv reagent, which exhibit an effect similar to that of degradation by enzymes, causes a more rapid progress of Penicillium spinulosum infection, thus reinforcing the notion that the inactivation of AG type II has an impact on the PCW integrity and the activation of plant defense responses ( Leszczuk et al, 2019 ).…”

Section: Ags Hydrolysis and Plant-microbe Interactionsmentioning

confidence: 95%

“…(B) Thermobacillus xylanolyticus ABF GH51, PDB code: 2VRQ. (C) Endo1,6GAL modeling of Colletotrichum lindemuthianum ( Villa-Rivera et al, 2017b ). (D) Aspergillus luchuensis ABF GH 54, PDB code: 1WD3.…”

Section: Degradation Of Ags By Enzymes Of Microorganismsmentioning

confidence: 99%

See 2 more Smart Citations

The Role of Arabinogalactan Type II Degradation in Plant-Microbe Interactions

et al. 2021

Self Cite

View full text Add to dashboard Cite

Arabinogalactans (AGs) are structural polysaccharides of the plant cell wall. A small proportion of the AGs are associated with hemicellulose and pectin. Furthermore, AGs are associated with proteins forming the so-called arabinogalactan proteins (AGPs), which can be found in the plant cell wall or attached through a glycosylphosphatidylinositol (GPI) anchor to the plasma membrane. AGPs are a family of highly glycosylated proteins grouped with cell wall proteins rich in hydroxyproline. These glycoproteins have important and diverse functions in plants, such as growth, cellular differentiation, signaling, and microbe-plant interactions, and several reports suggest that carbohydrate components are crucial for AGP functions. In beneficial plant-microbe interactions, AGPs attract symbiotic species of fungi or bacteria, promote the development of infectious structures and the colonization of root tips, and furthermore, these interactions can activate plant defense mechanisms. On the other hand, plants secrete and accumulate AGPs at infection sites, creating cross-links with pectin. As part of the plant cell wall degradation machinery, beneficial and pathogenic fungi and bacteria can produce the enzymes necessary for the complete depolymerization of AGs including endo-β-(1,3), β-(1,4) and β-(1,6)-galactanases, β-(1,3/1,6) galactanases, α-L-arabinofuranosidases, β-L-arabinopyranosidases, and β-D-glucuronidases. These hydrolytic enzymes are secreted during plant-pathogen interactions and could have implications for the function of AGPs. It has been proposed that AGPs could prevent infection by pathogenic microorganisms because their degradation products generated by hydrolytic enzymes of pathogens function as damage-associated molecular patterns (DAMPs) eliciting the plant defense response. In this review, we describe the structure and function of AGs and AGPs as components of the plant cell wall. Additionally, we describe the set of enzymes secreted by microorganisms to degrade AGs from AGPs and its possible implication for plant-microbe interactions.

show abstract

Section: Degradation Of Ags By Enzymes Of Microorganismssupporting

confidence: 68%

Section: Ags Hydrolysis and Plant-microbe Interactionsmentioning

confidence: 95%

See 1 more Smart Citation

The Role of Arabinogalactan Type II Degradation in Plant-Microbe Interactions

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Xylan is the second most abundant polysaccharide in PCW and consists of a backbone of β-(1,4)-D-xylose that can be branched by different glycosidic residues, such as L-arabinofuranose, L-arabinopiranose, L-rhamnose, D-glucuronic acid, and D-galacturonic acid, which can bind to the ends of the pectic polysaccharides and cellulose fibers [ 34 , 35 ]. In cultures with glucose as the carbon source, P0 and P1472 showed low levels of XYL activity ( Figure 2 A), in agreement with previous reports [ 14 , 26 ] and consistent with the CCR of hydrolytic enzyme-encoding genes in filamentous fungi [ 36 , 37 , 38 ].…”

Section: Resultssupporting

confidence: 92%

“…Previously, we found that two pathotypes of C. lindemuthianum (pathogenic P1472 and non-pathogenic P0) showed differential gene expression and/or secretion levels of pectin lyase, endo-1,4-β-D-xylanase, and β-galactosidase in cultures with pectin, polygalacturonic acid, arabinogalactan, xylan, and PCW from P. vulgaris as carbon sources, suggesting a relationship with their lifestyle differences [ 11 , 12 , 14 , 26 ]. Furthermore, these findings suggest the differential production of PCWDEs with biotechnological potential.…”

Section: Introductionmentioning

confidence: 99%

Differential Carbon Catabolite Repression and Hemicellulolytic Ability among Pathotypes of Colletotrichum lindemuthianum against Natural Plant Substrates

Díaz-Tapia,

Zavala-Páramo,

Villa-Rivera

et al. 2024

JoF

Self Cite

View full text Add to dashboard Cite

Colletotrichum lindemuthianum is a phytopathogenic fungus that causes anthracnose in common beans (Phaseolus vulgaris) and presents a great diversity of pathotypes with different levels of virulence against bean varieties worldwide. The purpose of this study was to establish whether pathotypic diversity is associated with differences in the mycelial growth and secretion of plant-cell-wall-degrading enzymes (PCWDEs). We evaluated growth, hemicellulase and cellulase activity, and PCWDE secretion in four pathotypes of C. lindemuthianum in cultures with glucose, bean hypocotyls and green beans of P. vulgaris, and water hyacinth (Eichhornia crassipes). The results showed differences in the mycelial growth, hemicellulolytic activity, and PCWDE secretion among the pathotypes. Glucose was not the preferred carbon source for the best mycelial growth in all pathotypes, each of which showed a unique PCWDE secretion profile, indicating different levels of carbon catabolite regulation (CCR). The pathotypes showed a high differential hemicellulolytic capacity to degrade host and water hyacinth tissues, suggesting CCR by pentoses and that there are differences in the absorption and metabolism of different monosaccharides and/or disaccharides. We propose that different levels of CCR could optimize growth in different host tissues and could allow for consortium behavior in interactions with bean crops.

show abstract

Differences in the expression profile of endo-β-(1,6)- d -galactanase in pathogenic and non-pathogenic races of Colletotrichum lindemuthianum grown in the presence of arabinogalactan, xylan or Phaseolus vulgaris cell walls

Cited by 2 publications

References 80 publications

The Role of Arabinogalactan Type II Degradation in Plant-Microbe Interactions

The Role of Arabinogalactan Type II Degradation in Plant-Microbe Interactions

Differential Carbon Catabolite Repression and Hemicellulolytic Ability among Pathotypes of Colletotrichum lindemuthianum against Natural Plant Substrates

Contact Info

Product

Resources

About