Differences in the expression of cell cycle genes in osteoblasts and endothelial cells cultured on the surfaces of Ti6Al4V and Ti6Al7Nb alloys

Walkowiak-Przybyło, Magdalena; Komorowski, Piotr; Walkowiak, Bogdan

doi:10.1002/jbm.a.35972

Cited by 3 publications

(6 citation statements)

References 17 publications

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“…However, contrary to these studies and our results, it has been reported that these two Ti alloy surfaces have no effect on proliferation of human osteosarcoma cells, − mesenchymal stem cells, Saos-2 osteoblasts, and EA.hy-926 endothelial cells, and even alloys containing Nb cause cytotoxicity in Saos cells . Of the two alloys containing nanotube films, it has been reported that alloys containing vanadium have better osteoblastic activities .…”

Section: Discussioncontrasting

confidence: 99%

Fibroblast Cell Responses to Vanadium and Niobium Titanium Alloys: A Biocompatibility Study

2023

ACS Omega

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The interactions of a biomaterial with tissues must be determined for the material to be fully compatible with the body for a long time. The tissue and environment where the material is implanted are highly affected by its content. Titanium-6Aluminum-4Vanadium is widely used in orthopedics and dentistry. Recently, Titanium-6Aluminum-7Niobium alloys have been studied because of Titanium-6Aluminum-4Vanadium toxicity, which may be caused by vanadium. The aim of this study was to determine whether Titanium-6Aluminum-4Vanadium and Titanium-6Aluminum-7Niobium affect fibroblast cell proliferation, mineralization, and collagen production and whether they change the expression of type 1 collagen and fibronectin genes. It was determined that the niobium-containing alloy increased cell proliferation and calcium mineralization compared with the vanadium-containing alloy (p < 0.05). However, the alloys did not cause changes in the expression of collagen type 1 or fibronectin in cells. The collagen content of the cells on the niobium-containing alloy was lower than that on both the vanadium-containing alloy and tissue culture plate surface (p < 0.05). The niobium-containing alloy was found to be superior to the vanadium-containing alloy in terms of cell proliferation and calcium mineralization. Furthermore, neither vanadium-containing alloy nor niobium-containing alloy implant materials altered gene expression. Although both alloys are considered compatible with bone tissue, it should be considered whether they are also biocompatible with fibroblast cells.

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Section: Discussioncontrasting

confidence: 99%

Fibroblast Cell Responses to Vanadium and Niobium Titanium Alloys: A Biocompatibility Study

2023

ACS Omega

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“…On the other hand, there were also specific differences that were clearly visible against the backdrop of a common pattern. The comparison was similar in all other areas of gene expression pattern and it confirms the existence of both specific and nonspecific cell responses to the stress resulting from exposure to artificial surfaces, which has been reported previously by the authors [ 22 , 23 , 24 ] and by others [ 25 , 26 ]. Unfortunately when taking into account transcriptomic analysis of cell response towards biomaterials, in particular titanium alloys, or PEEK, in current literature there is a lack of reports on this topic.…”

Section: Discussionsupporting

confidence: 88%

“…However, some of the genes differed in expression both in relation to the control cells and between cells exposed to individual biomaterials. It has been previously reported that cells are able to recognize and respond to stress resulting from contact with different artificial surfaces in a highly specific manner [ 22 , 23 , 24 ]. Analysis of the results obtained in this study confirms this phenomenon and brings to light several new observations.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Biological Evaluation of Biomaterials Used in Spinal and Orthopedic Surgery

Komorowski

Siatkowska²,

Kamińska

et al. 2020

Materials

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Biological acceptance is one of the most important aspects of a biomaterial and forms the basis for its clinical use. The aim of this study was a comprehensive biological evaluation (cytotoxicity test, bacterial colonization test, blood platelets adhesion test and transcriptome and proteome analysis of Saos-2 cells after contact with surface of the biomaterial) of biomaterials used in spinal and orthopedic surgery, namely, Ti6Al4V ELI (Extra Low Interstitials), its modified version obtained as a result of melting by electron beam technology (Ti6Al4V ELI-EBT), polyether ether ketone (PEEK) and polished medical steel American Iron and Steel Institute (AISI) 316L (the reference material). Biological tests were carried out using the osteoblasts-like cells (Saos-2, ATCC HTB-85) and bacteria Escherichia coli (DH5α). Results showed lack of cytotoxicity of all materials and the surfaces of both Ti6Al4V ELI and PEEK exhibit a significantly higher resistance to colonization with E. coli cells, while the more porous surface of the same titanium alloy produced by electron beam technology (EBT) is more susceptible to microbial colonization than the control surface of polished medical steel. None of the tested materials showed high toxicity in relation to E. coli cells. Susceptibility to platelet adhesion was very high for polished medical steel AISI 316L, whilst much lower for the other biomaterials and can be ranked from the lowest to the highest as follows: PEEK < Ti6Al4V ELI < Ti6Al4V ELI-EBT. The number of expressed genes in Saos-2 cells exposed to contact with the examined biomaterials reached 9463 genes in total (ranging from 8455 genes expressed in cells exposed to ELI to 9160 genes in cells exposed to PEEK). Whereas the number of differentially expressed proteins detected on two-dimensional electrophoresis gels in Saos-2 cells after contact with the examined biomaterials was 141 for PEEK, 223 for Ti6Al4V ELI and 133 for Ti6Al4V ELI-EBT. Finally, 14 proteins with altered expression were identified by mass spectrometry. In conclusion, none of the tested biomaterials showed unsatisfactory levels of cytotoxicity. The gene and protein expression analysis, that represents a completely new approach towards characterization of these biomaterials, showed that the polymer PEEK causes much more intense changes in gene and protein expression and thus influences cell metabolism.

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“…In addition to the amount of proteins adsorbed on the surface, their biological activity, which is connected, for example, with their conformation, may be changed [46,47]. Moreover, the size of the biological molecules and the time of contact with the biomaterial’s surface can determine the tissue–biomaterial interaction [48].…”

Section: Resultsmentioning

confidence: 99%

“…This may be due to the fact that the content of Si in the Si-DLC 4 coating is too high and it affects the process of cells’ division. The critical concentration of Si may be different for different cell lines and it may be associated with cellular adaptation [48,53]. Moreover, according to some authors, the high hydrophilicity of surface may inhibit the adhesion of cells due to the preferential adsorption of water molecules [54].…”

Section: Resultsmentioning

confidence: 99%

Surface Characteristics and Biological Evaluation of Si-DLC Coatings Fabricated Using Magnetron Sputtering Method on Ti6Al7Nb Substrate

et al. 2019

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Diamond-like carbon (DLC) coatings are well known as protective coatings for biomedical applications. Furthermore, the incorporation of different elements, such as silicon (Si), in the carbon matrix changes the bio-functionality of the DLC coatings. This has also been proven by the results obtained in this work. The Si-DLC coatings were deposited on the Ti6Al7Nb alloy, which is commonly used in clinical practice, using the magnetron sputtering method. According to the X-ray photoelectron spectroscopy (XPS) analysis, the content of silicon in the examined coatings varied from ~2 at.% up to ~22 at.%. Since the surface characteristics are key factors influencing the cell response, the results of the cells’ proliferation and viability assays (live/dead and XTT (colorimetric assays using tetrazolium salt)) were correlated with the surface properties. The surface free energy (SFE) measurements, Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the polarity and wettability of the surfaces examined increase with increasing Si concentration, and therefore the adhesion and proliferation of cells was enhanced. The results obtained revealed that the biocompatibility of Si-doped DLC coatings, regardless of the Si content, remains at a very high level (the observed viability of endothelial cells is above 70%).

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Differences in the expression of cell cycle genes in osteoblasts and endothelial cells cultured on the surfaces of Ti6Al4V and Ti6Al7Nb alloys

Cited by 3 publications

References 17 publications

Fibroblast Cell Responses to Vanadium and Niobium Titanium Alloys: A Biocompatibility Study

Fibroblast Cell Responses to Vanadium and Niobium Titanium Alloys: A Biocompatibility Study

Comprehensive Biological Evaluation of Biomaterials Used in Spinal and Orthopedic Surgery

Surface Characteristics and Biological Evaluation of Si-DLC Coatings Fabricated Using Magnetron Sputtering Method on Ti6Al7Nb Substrate

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