Differences in the drought stress response ofDREB2andCAT1genes and evaluation of related physiological parameters in some bread wheat cultivars

Abstract

Abiotic stresses such as drought are among the most significant environmental stress causes in economically important crop plants including wheat (Triticum aestivum L.) and improving their yield is a major goal of plant breeding. In this study, we analysed expression of dehydration responsive element binding factor (DREB2), and an important antioxidant enzyme gene CAT1 in flag leaf of three bread wheat cultivars named Kavir, Kalheydari and Shahpasand under drought stress by RT-qPCR. In another section, physiological parameters including catalase enzyme (EC 1.11.1.6), relative water content (RWC) and chlorophyll content (a,b and total) were analysed. The cultivars were planted in a randomized complete block design with three replicates in normal and drought stress conditions on experimental field in 2013. Differential expression patterns of the genes DREB2 and CAT1 were observed in cultivars. The observed trend was the high induction in the expression of genes under drought stress. The expression of DREB2 was more than CAT1 in drought stress. The relative genes expression showed significant correlation with the catalase, RWC and chlorophyll b. Under drought stress, Kavir had higher expression of DREB2 and CAT1, activity of catalase, RWC, chlorophyll content (a, b and total) than the other cultivars. According to increasing of drought tolerance through the expression of these genes, it can be concluded that transferring of these genes may enhance drought tolerance in high-yield wheat cultivars.

Screening of worldwide cowpea collection to drought tolerant at a germination stage

“…Worldwide agricultural production has been limited by several environmental constraints in the form of abiotic stresses, which affects plants growth, metabolism and development (Eftekhari et al, 2017;Muscolo et al, 2014). Water scarcity is currently one of the most severe limitations of plant development and production (Eftekhari et al, 2017;Jain and Saxena, 2016). The predicted temperature increase and rainfall decrease will be responsible for more frequent drought periods, mainly in the Mediterranean region including the Iberian Peninsula (Kröner et al, 2017).…”

Drought and salinity stress response in wheat: physiological andTaNACgene expression analysis in contrasting Egyptian wheat genotypes

“…Furthermore, the sensitive genotypes recorded the lowest means for all studied traits, which may indicate that those genotypes showed a lower capacity to accord with stress conditions. In the same context, (Eftekhari et al 2017;Kamoshita et al 2000;Schonfeld et al 1988) revealed that RWC for tolerant cultivar retain major amount of water than the non-tolerant and RWC reduced significantly under drought stress conditions. Under drought conditions, the decreasing in RWC fundamentally related with the capacity of more tolerant genotypes to better absorb soil, water and to prevent water loss through stomata (Keyvan et al 2010).…”

Transcriptome changes induced by drought stress in Jerusalem artichoke (Helianthus tuberosus L.) and weighted gene co-expression network analysis (WGCNA)

“…DREB2 and DREB2a were also highly expressed in both varieties of Jerusalem artichoke, particularly in QY3, in which the expression of DREB2 was extremely high. It has been proven that DREB2 was closely related to drought stress in wheat [42], A. thaliana [43] and mustard [44]. Compared with the DREB family, which had only a few genes, we identi ed a number of WRKY transcription factors in the Jerusalem artichoke transcriptome data.…”