Differences in muscarinic-receptor agonist–, oxytocin-, and prostaglandin-induced uterine contractions

Dittrich, Ralf; Mueller, Andreas; Oppelt, Patricia G.; Hoffmann, Inge; Beckmann, Matthias W.; Maltaris, Theodoros

doi:10.1016/j.fertnstert.2008.08.117

Cited by 28 publications

(15 citation statements)

References 53 publications

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“…Oxytocin and PGF2α have been reported to play an important role in the myometrial contraction [37]. Oxytocin-induced myometrial contraction has been shown in estrogen-primed non-pregnant swine uteri [38,39]. Activation of the oxytocin and PGF2α receptors which are coupled to G protein alpha(q) stimulates uterine contraction through activating the phospholipase C/Ca 2+ dependent pathway, while activation of the muscarinic receptor which is coupled to G protein alpha(i) potentiates contraction through inhibiting the cAMP production [40].…”

Section: Discussionmentioning

confidence: 99%

In-Vitro effect of Ficus deltoidea on the contraction of isolated rat’s uteri is mediated via multiple receptors binding and is dependent on extracellular calcium

Salleh

Ahmad

2013

BMC Complement Altern Med

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BackgroundFicus deltoidea, is a perennial herb that is used to assist labor, firm the uterus post-delivery and to prevent postpartum bleeding. In view of its claimed uterotonic action, the mechanisms underlying plant’s effect on uterine contraction were investigated.MethodsAdult female SD rats were injected with 2 mg/kg 17β-oestradiol (E2) to synchronize their oestrous cycle. A day after injection, uteri were removed for in-vitro contraction studies. The dose dependent effect of Ficus deltoidea aqeous extract (FDA) on the tension produced by the isolated rat’s uteri was determined. The effects of atropine (2×10-8 M), atosiban (0.5 IU), THG113.31 (10 μM), oxodipine (0.25 mM), EDTA (1 mM), 2-amino-ethoxy-diphenylborate (2-APB) (40 mM) and thapsigargin (1 mM) on the maximum force of contraction (Emax) achieved following 2 mg/ml FDA administration were also investigated.ResultsFDA induced in-vitro contraction of the isolated rat’s uteri in a dose-dependent manner. Administration of atropine, atosiban and THG113.31 reduced the Emax with atosiban having the greatest effect. The Emax was also reduced following oxodipine and EDTA administration. There was no significant change observed following 2-APB administration. Thapsigargin, however, augmented Emax.ConclusionsFDA-induced contraction of the isolated rat’s uteri is mediated via multiple uterotonin receptors (muscarinic, oxytocin and prostaglandin F2α) and was dependent on the extracellular Ca2+. Contraction, however, was not dependent on the Ca2+ release from the internal stores. This in-vitro study provides the first scientific evidence on the claimed effect of Ficus Deltoidea on uterine contraction.

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Section: Discussionmentioning

confidence: 99%

In-Vitro effect of Ficus deltoidea on the contraction of isolated rat’s uteri is mediated via multiple receptors binding and is dependent on extracellular calcium

Salleh

Ahmad

2013

BMC Complement Altern Med

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“…therefore it is possible that uterine muscle contractions induced by FDD and FDA stimulate endogenous myometrial synthesis of PGF2α that later bound to PGF2α receptors to activate downstream signalling (Naguib & Vivi Noryati 2013). Oxytocin is also able to stimulate endometrial secretion of endogenous PGF2α to effectively increase the uterine contractions by promoting a more forceful contraction (Carnahan et al 1996;Dittrich et al 2009, Arrowsmith et al 2010. Other than that, estrogen also has been reported to increase PGF2α production that in turn has direct stimulation on uterine contractions (Egarter & husslein 1982).…”

Section: Discussionmentioning

confidence: 99%

Mechanism Identification of Ficus Deltoidea Aqueous Extract in Rat Uterine Contractions

Ibrahim

Derased

Zainudin

et al. 2018

JSKM

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Ficus deltoidea or 'mas cotek,' is a uterotonic herb traditionally consumed by women to improve menstrual circulation, assist labour, remove retained placenta and treat postpartum bleeding. The aim of the study was to elucidate the mechanism of F. deltoidea in uterine contraction. Crude extracts from 2 different variants of F. deltoidea were used in the study; F. deltoidea var. Deltoidea (FDD) and F. deltoidea var. Angustifolia (FDA). This study was conducted ex vivo on the strips of isolated rats uterus treated with either FDD or FDA aqueous extract with increasing concentrations ranging from 10 µg/ ml until 1280 µg/ml at time intervals of 5 minutes between doses. The frequency and intensity of the uterine contractions were monitored via Powerlab software. Maximum contractions for both extracts were identified, recorded and the uterine strips samples at maximum contraction were selected and homogenized in order determine the role of prostaglandin F2α (PGF2α) in the mechanism of uterine contraction. Other than that, phosphorylated 42/44 (p42/44) of mitogen activated protein kinase (MAPK) expression was also detected via immunoblotting. The results showed that the maximum contraction induced by FDD was at the concentration of 320 µg/ml, whereas for FDA was at 960 µg/ml. Both FDD and FDA increased the intensity of uterine strips contractions and there were notable trend of increased PGF2α expression as well. Further analysis revealed that the uterine contractions involved the MAPK pathway through the phosphorylation of p42/44 protein. In conclusion, Ficus deltoidea of both variants have the ability to stimulate uterine contraction through the mechanism of MAPK pathway.

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“…Rhythmic contractions on stimulation with oxytocin were described after perfusion of whole swine uteri,11 in strips of the vaginal wall in the rabbit,12 and analyzed in detail in strips of human myometrium 13. The Gq coupled oxytocin receptor is coupled to various transduction chains including direct action on cell contraction via rhoA and the indirect cGMP–NO pathway 14…”

Section: Discussionmentioning

confidence: 99%

Ex vivo pharmacology of surgical samples of the uterosacral ligament. Part I: Effects of carbachol and oxytocin on smooth muscle

Drews

Renz²,

Busch

et al. 2012

Neurourology and Urodynamics

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Differences in muscarinic-receptor agonist–, oxytocin-, and prostaglandin-induced uterine contractions

Cited by 28 publications

References 53 publications

In-Vitro effect of Ficus deltoidea on the contraction of isolated rat’s uteri is mediated via multiple receptors binding and is dependent on extracellular calcium

In-Vitro effect of Ficus deltoidea on the contraction of isolated rat’s uteri is mediated via multiple receptors binding and is dependent on extracellular calcium

Mechanism Identification of Ficus Deltoidea Aqueous Extract in Rat Uterine Contractions

Ex vivo pharmacology of surgical samples of the uterosacral ligament. Part I: Effects of carbachol and oxytocin on smooth muscle

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