Differences in IgG1 and IgG2 responses of cattle infected with Coxiella burnetii and following vaccination

Schmeer, N.; Krauss, H; Lohrbach, W; Wiegand, D

doi:10.1016/0147-9571(86)90080-9

Cited by 12 publications

(6 citation statements)

References 4 publications

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“…An important consideration is which phase of C. burnetii to use. Whilst experiments in laboratory animals have demonstrated the superiority of phase I vaccines in affording protection against challenge (18), large scale commercial production of antigen is easier and safer using organisms in phase II (23). The particular strain of the organism would appear to be of little consequence given the proven ability of individual strains to induce complete reciprocal cross protection, at least in guinea pigs (18).…”

Section: Preventionmentioning

confidence: 99%

“…Of the more sensitive and versatile tests now available the enzyme-linked immunosorbent assay (ELISA) is particularly valuable for routine serum analysis and for isotype analysis of the antibody response. Recently, considerable advances have been made in the development and application of an ELISA for detection and sub-isotypic discrimination of antibodies to C. burnetii (23,24). Notwithstanding criticisms of possible allotypic bias in this type of test (11) it has the particular pragmatic advantage that, in cattle, it can differentiate between the essentially IgG~ response to infection and the predominantly IgG: response to vaccination.…”

Section: Preventionmentioning

confidence: 99%

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Clinical aspects and prevention of Q fever in animals

Aitken

1989

Eur J Epidemiol

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The natural reservoir of Coxiella burnetii encompasses many free-living vertebrates but the major risk of human infection arises through contact with infected ruminant livestock and their contaminated products. The organism has a remarkable affinity for the ruminant placenta and mammary gland but the great majority of naturally-occurring infections are asymptomatic. However, the potential of C. burnetii to cause abortion has been demonstrated experimentally and observed in the field while more recent evidence has implied a contributory role in bovine infertility. Empirical vaccines incorporating inactivated whole cells of C. burnetii or derivatives have induced varying degrees of protection of cattle and sheep against both natural and experimental challenge but, in some cases, severe reactions have occurred at inoculation sites. Modifications in processes of antigen preparation seem to overcome this problem. Discrimination between antibodies resulting from natural infection and those induced by vaccination is possible using ELISAs with specificity for individual immunoglobulin isotypes.

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Section: Preventionmentioning

confidence: 99%

Section: Preventionmentioning

confidence: 99%

Clinical aspects and prevention of Q fever in animals

Aitken

1989

Eur J Epidemiol

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“…From previous vaccination experiments, a pool of serum samples from 10 vaccinated cattle was used which had preinoculation titers derived from natural C. burnetii infection prior to vaccination with a Formalin-inactivated whole-cell antigen of C. burnetii (22). Fifty-two serum samples from nonvaccinated cattle with naturally occurring C. burnetii infections (different breeds, ages, and sexes originating from the Federal Republic of Germany and the United States) were randomly selected from a serum bank available from previous investigations (18,20,22,23).…”

Section: Downloaded Frommentioning

confidence: 99%

“…Previous investigations with IgG1-and IgG2-specific ELISA, using a whole-cell antigen, demonstrated that cattle vaccinated with a commercially available, Formalin-inactivated Q fever vaccine predominantly synthesized C. burnetii-specific IgG2. In contrast, naturally infected cattle preferentially produced IgGl (18,20,22). Therefore, we investigated to what extent different antigens, such as SP27 and LPS, induce production of different IgG subclasses.…”

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confidence: 99%

Enzyme-linked immunosorbent fluorescence assay and high-pressure liquid chromatography for analysis of humoral immune responses to Coxiella burnetti proteins

et al. 1988

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A microtiter enzyme-linked immunosorbent fluorescence assay based on alkaline phosphatase conjugate and 4-methylumbelliferyl phosphate as fluorogenic substrate was developed and adapted to quantitatively analyze immunoglobulin G subclass 1 (IgGl) and IgG2 responses of vaccinated and infected cattle to proteins of Coxiella burnetii. The enzyme-linked immunosorbent fluorescence assay surpassed the conventional enzymelinked immunosorbent assay with a 50-fold-higher sensitivity and a broader range of linear dose-response signals. Antigens of C. burnetii were purified by sodium dodecyl sulfate extraction and molecular-sieve high-pressure liquid chromatography. The purified 14-, 27-, and 30-kilodalton proteins were used as antigens without any further treatment. Vaccination with either chloroform-methanol-extracted cell residues of C. burnetii or the 27-kilodalton major surface protein evoked an early IgG2 response to the 27-kilodalton protein (2 weeks after immunization), whereas IgG2 to lipopolysaccharides of C. burnetii was detected only in the late phase (13 weeks after immunization). These results may have implications for the serodiagnosis of acute and chronic Q fever. IgGl against these antigens was induced solely by naturally occurring C. burnetii infections, indicating that infected cattle can be distinguished from vaccinated cattle by using the enzyme-linked immunosorbent fluorescence assay and SP27 antigen.

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“…Indirekte Immunperoxidase-Technik (IPOD) Die Durchfuhrung des Immunperoxidaseverfahrens zum Nachweis Coxiellen-spezifischer Antikorper erfolgte bis auf folgende Modifikationen wie kurzlich beschrieben (38) (35,36). Auch bei Coxiellen-infizierten Menschen, sowie Chlamydien-infizierten Tauben lieR sich nicht-komplementbindendes IgM (31,33,49) nur mit IgM-spezifischen oder mit allen Immunglobulinen reagierenden IgG(H+L)-spezifischen ELISAs bzw.…”

Section: Agargelprazipitationstest (Agpt)unclassified

Nahweis von Antikörpern gegen Chlamydia psittaci und Coxiella burnetii bei Hunden und Katzen: Vergleich zwischen Enzymimmuntest, Immunperoxidase‐Technik, Komplementbindungsreaktion und Agargelpräzipitationstest

Werth

Schmeer

Müller

et al. 1987

Journal of Veterinary Medicine, Series B

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Summary Detection of Antibodies to Chlamydia psittaci and Coxiella burnetii in dogs and cats: Comparison between Enzyme‐linked Immunosorbent Assay, Immunoperoxidase‐Technique, Complement Fixation Test and Immunodiffusion Test An enzyme‐linked immunosorbent assay was applied for the first time to detect antibodies against Chlamydia psittaci and Coxiella burnetii in 1127 serum samples of dogs and 108 serum samples of cats originating from the Federal Republic of Germany. In comparison to the immunodiffusion test, the immunoperoxidase technique and the complement fixation test, the ELISA proved to be specific and superseded the other tests in sensitivity and practicability. Using a single serum dilution of 1:100 and an automated photometer, specific antibody levels of large numbers of sera could be determined quantitatively in a short time. In dogs, the ELISA revealed seroprevalences of 20% and 13% against Chlamydia psittaci and Coxiella burnetii, respectively. Of the cats 19% reacted against chlamydia and 26% against coxiella. Zusammenfassung In der vorliegenden Studie wurden 1127 Hundeseren und 108 Katzenseren aus der Bundesrepublik Deutschland auf ihren Gehalt an Antikörper gegen Chlamydia psittaci und Coxiella burnetii untersucht, wobei erstmals ein Enzyme‐linked Immunosorbent Assay (ELISA) zur Anwendung kam. Im Vergleich zu dem Agargelpräzipitationstest, der indirekten Immunperoxidase‐Technik und der Komplementbindungsreaktion erwies sich der ELISA bei gleicher Spezifität den anderen Techniken an Sensitivität und Praktikabilität als deutlich überlegen. Durch Anwendung von nur einer Serumverdünnung von 1:100 und eines automatischen Photometers konnte mit dem ELISA eine quantitative Bestimmung des spezifischen Antikörperspiegels in einer großen Anzahl von Seren in kürzester Zeit durchgeführt werden. Anhand der im ELISA ermittelten Ergebnisse ließen sich in der Hundepopulation eine Seroprävalenz von 20% gegen Chlamydia psittaci und 13% gegen Coxiella burnetii feststellen. In der Katzenpopulation lag die Seroprävalenz bei 19% (Chlamydien) und 26% (Coxiellen).

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Differences in IgG1 and IgG2 responses of cattle infected with Coxiella burnetii and following vaccination

Cited by 12 publications

References 4 publications

Clinical aspects and prevention of Q fever in animals

Clinical aspects and prevention of Q fever in animals

Enzyme-linked immunosorbent fluorescence assay and high-pressure liquid chromatography for analysis of humoral immune responses to Coxiella burnetti proteins

Nahweis von Antikörpern gegen Chlamydia psittaci und Coxiella burnetii bei Hunden und Katzen: Vergleich zwischen Enzymimmuntest, Immunperoxidase‐Technik, Komplementbindungsreaktion und Agargelpräzipitationstest

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