Differences in genomic architecture between two distinct geographical strains of the blood fluke Schistosoma japonicum reveal potential phenotype basis

Gobert, Geoffrey N.; You, Hong; Jones, Malcolm K.; McInnes, Russell; McManus, Donald P.

doi:10.1016/j.mcp.2012.08.005

Cited by 4 publications

(5 citation statements)

References 80 publications

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“…Given these antigens are expressed in the gastrodermis or tegument, the process of vesicle-mediated transport may play an important role in the localization of these proteins. Previously, it has been shown that some of the components associated with vesicle-mediated transport have lower copy number in SjP compared with SjC worms ( Gobert et al, 2013 ), which may impact on the exposure of these antigens to the host. Further, the rSjSTIP1- and rSjPPase-ELISAs showed a sensitivity of 80% and 90%, respectively, on testing a small number of patient sera with SjC-infection ( Chen et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

A Parallel Comparison of Antigen Candidates for Development of an Optimized Serological Diagnosis of Schistosomiasis Japonica in the Philippines

Cai

Weerakoon

et al. 2017

EBioMedicine

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Schistosoma japonicum is stubbornly persistent in China and the Philippines. Fast and accurate diagnostic tools are required to monitor effective control measures against schistosomiasis japonica. Promising antigen candidates for the serological diagnosis of schistosomiasis japonica have generally been identified from the Chinese strain of S. japonicum. However, the Chinese (SjC) and Philippine (SjP) strains of S. japonicum express a number of clear phenotypic differences, including aspects of host immune responses. This feature thereby emphasized the requirement to determine whether antigens identified as having diagnostic value for SjC infection are also suitable for the diagnosis of SjP infection. In the current study, 10 antigens were selected for comparison of diagnostic performance of the SjP infection using ELISA. On testing of sera from 180 subjects in the Philippines, SjSAP4 exhibited the best diagnostic performance with 94.03% sensitivity and 98.33% specificity using an optimized serum dilution. In another large scale testing with 412 serum samples, a combination (SjSAP4 + Sj23-LHD (large hydrophilic domain)) provided the best diagnostic outcome with 87.04% sensitivity and 96.67% specificity. This combination could be used in future for serological diagnosis of schistosomiasis in the Philippines, thereby representing an important component for monitoring integrated control measures.

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Section: Discussionmentioning

confidence: 99%

A Parallel Comparison of Antigen Candidates for Development of an Optimized Serological Diagnosis of Schistosomiasis Japonica in the Philippines

Cai

Weerakoon

et al. 2017

EBioMedicine

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“…Resulting infection burdens in terms of egg and worm pair counts were higher with SjC, consistent with previous findings (Hope et al 1996). These and other phenotypical differences between the SjC and SjP geographical strains have been investigated using transcriptomic and comparative genomic methods (Moertel et al 2006; Gobert et al 2013).…”

Section: Discussionmentioning

confidence: 99%

A novel duplex ddPCR assay for the diagnosis of schistosomiasis japonica: proof of concept in an experimental mouse model

et al. 2017

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The current World Health Organization strategic plan targets the elimination of schistosomiasis as a public health problem by 2025 and accurate diagnostics will play a pivotal role in achieving this goal. DNA-based detection methods provide a viable alternative to some of the commonly used tests, notably microscopy and serology, for the diagnosis of schistosomiasis. The detection of parasite cell-free DNA in different clinical samples is a recent valuable advance, which provides significant benefits for accurate disease diagnosis. Here we validated a novel duplex droplet digital PCR assay for the diagnosis of Chinese (SjC) and Philippine (SjP) strains of Schistosoma japonicum infection in a mouse model. The assay proved applicable for both SjC and SjP infections and capable of detecting infection at a very early intra-mammalian stage in conveniently obtainable samples (urine and saliva) as well as in serum and feces. The target DNA copy numbers obtained in the assay showed a positive correlation with the infection burden assessed by direct traditional parasitology. The potential to detect parasite DNA in urine and saliva has important practical implications for large-scale epidemiological screening programmes in the future, particularly in terms of logistical convenience, and the assay has the potential to be a valuable additional tool for the diagnosis of schistosomiasis japonica.

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“…HSBs represent large sections of a scaffold which share at least two markers between the two species with a conserved order, allowing assignment to a particular chromosome. Differences in HSBs between the two species were identified as "Evolutionary Breakpoint Regions" and were associated with sites where intra-chromosomal rearrangements can occur; with some of these sites subsequent identified in a study of two geographical strains of S. japonicum [9], supporting their in silico identification.…”

Section: Introductionmentioning

confidence: 73%

“…This provides a useful resource to allow the examination of broad chromosomal rearrangements that, for example, may have occurred between species or between geographical isolates. The latter was reported using comparative genomic hybridizations for S. japonicum endemic to mainland China and the Philippines but the study was limited and rudimentary due to the application of an incomplete microarray-based approach [9]. Finally the updated version of the S. mansoni genome also included extensive RNAseq analysis to profile the transcriptional status of cercariae, 3 h schistosomules, 24 h schistosomules and adult parasites.…”

Section: Introductionmentioning

confidence: 99%

Gaining biological perspectives from schistosome genomes

Gobert

You

McManus

2014

Molecular and Biochemical Parasitology

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Differences in genomic architecture between two distinct geographical strains of the blood fluke Schistosoma japonicum reveal potential phenotype basis

Cited by 4 publications

References 80 publications

A Parallel Comparison of Antigen Candidates for Development of an Optimized Serological Diagnosis of Schistosomiasis Japonica in the Philippines

A Parallel Comparison of Antigen Candidates for Development of an Optimized Serological Diagnosis of Schistosomiasis Japonica in the Philippines

A novel duplex ddPCR assay for the diagnosis of schistosomiasis japonica: proof of concept in an experimental mouse model

Gaining biological perspectives from schistosome genomes

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