Differences in gene expression in the proliferative human endometrium

Yanaihara, Atsushi; Otsuka, Yukiko; Iwasaki, Shinji; Aida, Tadateru; Tachikawa, Tetsuhiko; Irie, Taro; Okai, Takashi

doi:10.1016/j.fertnstert.2004.11.032

Cited by 37 publications

(24 citation statements)

References 45 publications

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“…Endometrial transcriptomic studies have covered the identification of the physiological endometrial profile throughout the menstrual cycle with special attention to the WOI (Carson et al 2002;Kao et al 2002;Borthwick et al 2003;Riesewijk et al 2003;Horcajadas et al 2004a,b;Ponnampalam et al 2004;Mirkin et al 2005;Puynadeera et al 2005;Yanaihara et al 2005;Critchley et al 2006;Talbi et al 2006;Haouzi et al 2009a;Kuokkanen et al 2010;Tseng et al 2010;Van Verenbergh et al 2010;Díaz-Gimeno et al 2011;Revel et al 2011). Endometrial transcriptomic profiles have also been investigated under different controlled ovarian stimulation protocols (Simon et al 2005;Horcajadas et al 2008), or in the context of unexplained pathologies such as recurrent implantation failure (Tapia et al 2008;Koler et al 2009;Altmäe et al 2010), or other endometrial conditions (Table 1) (Habermann et al 2011;Matsuzaki 2011).…”

Section: Transcriptomics Of the Endometriummentioning

confidence: 99%

“…Most of these studies investigate the transcriptomic signature in the whole endometrial tissue without separating the different compartments. However, in some studies, laser capture micro-dissection has facilitated specific compartment gene expression profiles (Yanaihara et al 2005;Evans et al 2012;Ulbrich et al 2013). Even the specific profiles for stromal cells and glands at different depths in the endometrium have been reported (Gaide Chevronnay et al 2009).…”

Section: Transcriptomics Of the Endometriummentioning

confidence: 99%

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Human Endometrial Transcriptomics: Implications for Embryonic Implantation

Gómez

Ruiz-Alonso

Miravet

et al. 2015

Cold Spring Harb Perspect Med

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Human endometrium has been extensively investigated in the search for markers capable of predicting its receptive status. The completion of the Human Genome Project has triggered a rapid development of new fields in molecular biology, the "transcriptomics" being a major turning point in the knowledge acquisition of endometrial receptivity. Based on this, a customized Endometrial Receptivity Array (ERA) has been developed, which is capable of identifying the genomic signature of receptivity. This diagnostic tool showed that the window of implantation (WOI) is displaced in one out of four patients with implantation failure, allowing the identification of their personalized WOI. This strategy allows performing a personalized embryo transfer ( pET) on the day in which the endometrium is receptive. The combination of a systems biology approach and next-generation sequencing will overcome the limitations of microarrays, and will, in the future, allow elucidation of the mechanisms involved in embryo implantation.T he endometrium, which lines the inside of the uterine cavity, undergoes cyclic changes that are regulated by ovarian steroids. It can be subdivided into the basal layer that is responsible for its regenerative capacity, and the functional layer that undergoes proliferation, secretion, and tissue degeneration every month from menarche to menopause. Its aim is to prepare the optimal moment for embryonic implantation known as the window of implantation (WOI).The endometrial cycle comprises the menstrual, proliferative, and secretory phases. The proliferative phase, which corresponds to the ovarian follicular phase and increased production of estrogens, lasts until ovulation occurs. During this stage, the increasing estrogen levels cause the proliferation of the stromal cells as well as the elongation of the spiral arteries. After ovulation, with the appearance of progesterone secreted by the granulosa-luteal cells, the secretory phase begins. If implantation does not occur, the secretory phase ends, and the corpus luteum degenerates. Menstruation occurs owing to the drop of estrogen and progesterone, which resets the endometrium until pregnancy

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Section: Transcriptomics Of the Endometriummentioning

confidence: 99%

Section: Transcriptomics Of the Endometriummentioning

confidence: 99%

Human Endometrial Transcriptomics: Implications for Embryonic Implantation

Gómez

Ruiz-Alonso

Miravet

et al. 2015

Cold Spring Harb Perspect Med

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show abstract

“…4. Preliminary studies using this technique have shown that it holds considerable promise to improve our understanding of the interactions between different cellular compartments in endometrium (Yanaihara et al 2005). Although microarray techniques have become extremely powerful tools, many cellular functions require alterations in proteins that are not reflected in steady-state RNA levels.…”

Section: Microarray Studies Of Endometriummentioning

confidence: 99%

Large-scale gene expression studies of the endometrium: what have we learnt?

Sherwin¹,

Catalano²,

Sharkey³

2006

Reproduction

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The endometrium is a dynamic tissue that undergoes coordinated changes under the influence of steroid hormones. This results in proliferation and differentiation culminating in a receptive state, followed by menstruation and endometrial repair. These functions involve complex interactions between the epithelium, stroma and leucocytes in the endometrium. Understanding the underlying causes of endometrial disorders, such as infertility, endometriosis and heavy menstrual bleeding, therefore represents a considerable challenge. Recently developed techniques, such as differential display and DNA microarrays permit the abundance of thousands of mRNA transcripts within cells or tissues to be measured simultaneously. This provides a new approach to understanding the complex interactions that underlie both healthy and disease states. Responses of the endometrium to hormones or drugs can be studied and the response of the system as an integrated whole can be assessed. Comparisons of endometrium from healthy women and those with endometrial dysfunction have advanced our understanding of key areas of endometrial physiology, including infertility, receptivity, endometriosis and cancer. Using this approach, novel genes controlling specific endometrial functions like receptivity have been identified for functional testing. This paper will review the impact of these techniques for transcript profiling on our understanding of selected areas of endometrial biology and discuss the potential applications in future.

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“…Also different endometrial cellular compartments have been analysed, demonstrating specific cell-type gene expression profiles (Yanaihara et al, 2005;Evans et al, 2012;Ulbrich et al, 2013). In all of these studies stromal and epithelial fractions were isolated by laser capture microdissection, and the mRNA signatures were related to the menstrual cycle day (Yanaihara et al, 2005;Evans et al, 2012).…”

Section: Transcriptomics Of Human Endometriummentioning

confidence: 99%

“…In all of these studies stromal and epithelial fractions were isolated by laser capture microdissection, and the mRNA signatures were related to the menstrual cycle day (Yanaihara et al, 2005;Evans et al, 2012). In addition, Evans' group compared Affymetrix and Agilent microarray platforms, demonstrating concordance in their results (Evans et al, 2012).…”

Section: Transcriptomics Of Human Endometriummentioning

confidence: 99%

Transcriptomics of the human endometrium

Díaz-Gimeno

Ruiz-Alonso²,

Blesa³

et al. 2014

Int. J. Dev. Biol.

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During the mid-secretory phase, the endometrium acquires the receptive phenotype, which corresponds to the only period throughout the endometrial cycle in which embryo implantation is viable. Endometrial receptivity is a crucial process and even more important in Assisted Reproductive Technologies (ART) where embryo-endometrial synchronization is coordinated through embryo transfer timing. Over the last decade, transcriptomic analyses performed on the human endometrium have shown that specific genomic signatures can be used to successfully phenotype different phases of the menstrual cycle including the receptive stage, independently of the histological appereance of the endometrial tissue. In this paper, we review current evidence demonstrating that endometrial transcriptomics objectively identifies the implantation window in a personalized manner, opening the field for the diagnosis of the endometrial factor in ART and moving to stratified medicine at this level, using microarray technology and soon high-throughput next generation sequencing coupled with functional and systems genomics approach.

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Differences in gene expression in the proliferative human endometrium

Cited by 37 publications

References 45 publications

Human Endometrial Transcriptomics: Implications for Embryonic Implantation

Human Endometrial Transcriptomics: Implications for Embryonic Implantation

Large-scale gene expression studies of the endometrium: what have we learnt?

Transcriptomics of the human endometrium

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