Transcriptomics of the human endometrium

Díaz-Gimeno, Patricia; Ruiz-Alonso, María; Blesa, David; Simón, Carlos

doi:10.1387/ijdb.130340pd

Cited by 58 publications

(43 citation statements)

References 100 publications

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“…Microarray-based expression profiling has technical limitations because it is limited by the nature of the probes included in the given platform as well as their sensitivity and specificity (Reis-Filho 2009;Díaz-Gimeno et al 2014). Nowadays, next generation sequencing (NGS) is an emerging technology that allows measurement of gene expression by RNA sequencing (RNA-seq).…”

Section: New Perspectives: Next-generation Sequencingmentioning

confidence: 99%

“…Nowadays, next generation sequencing (NGS) is an emerging technology that allows measurement of gene expression by RNA sequencing (RNA-seq). This new technology is capable of sequencing all the mRNAs present in a given sample, even the 25% of genes with low expression that remain undetected with standard microarray technologies (Mane et al 2009;Wang et al 2009;Díaz-Gimeno et al 2014).…”

Section: New Perspectives: Next-generation Sequencingmentioning

confidence: 99%

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Human Endometrial Transcriptomics: Implications for Embryonic Implantation

Gómez

Ruiz-Alonso

Miravet

et al. 2015

Cold Spring Harb Perspect Med

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Human endometrium has been extensively investigated in the search for markers capable of predicting its receptive status. The completion of the Human Genome Project has triggered a rapid development of new fields in molecular biology, the "transcriptomics" being a major turning point in the knowledge acquisition of endometrial receptivity. Based on this, a customized Endometrial Receptivity Array (ERA) has been developed, which is capable of identifying the genomic signature of receptivity. This diagnostic tool showed that the window of implantation (WOI) is displaced in one out of four patients with implantation failure, allowing the identification of their personalized WOI. This strategy allows performing a personalized embryo transfer ( pET) on the day in which the endometrium is receptive. The combination of a systems biology approach and next-generation sequencing will overcome the limitations of microarrays, and will, in the future, allow elucidation of the mechanisms involved in embryo implantation.T he endometrium, which lines the inside of the uterine cavity, undergoes cyclic changes that are regulated by ovarian steroids. It can be subdivided into the basal layer that is responsible for its regenerative capacity, and the functional layer that undergoes proliferation, secretion, and tissue degeneration every month from menarche to menopause. Its aim is to prepare the optimal moment for embryonic implantation known as the window of implantation (WOI).The endometrial cycle comprises the menstrual, proliferative, and secretory phases. The proliferative phase, which corresponds to the ovarian follicular phase and increased production of estrogens, lasts until ovulation occurs. During this stage, the increasing estrogen levels cause the proliferation of the stromal cells as well as the elongation of the spiral arteries. After ovulation, with the appearance of progesterone secreted by the granulosa-luteal cells, the secretory phase begins. If implantation does not occur, the secretory phase ends, and the corpus luteum degenerates. Menstruation occurs owing to the drop of estrogen and progesterone, which resets the endometrium until pregnancy

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Section: New Perspectives: Next-generation Sequencingmentioning

confidence: 99%

Section: New Perspectives: Next-generation Sequencingmentioning

confidence: 99%

Human Endometrial Transcriptomics: Implications for Embryonic Implantation

Gómez

Ruiz-Alonso

Miravet

et al. 2015

Cold Spring Harb Perspect Med

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“…These original studies have been refined over the years using immunohistochemical (IHC) markers of cell proliferation, coupled with expression of cell cycle regulated proteins and steroid receptors that together with quantitative approaches in staged endometrial biopsies confirmed the Noyes staging and form the basis of current understanding [6]. Similarly, global gene expression studies of the whole human endometrium defined proliferative, early secretory, mid-secretory and late secretory endometrium into specific categories based on transcriptional and microRNA profiles, indicating the significance of the coordinated sequence of molecular events in response to sex steroids [7–10]. …”

Section: Introductionmentioning

confidence: 93%

Xenografted tissue models for the study of human endometrial biology

Kuokkanen

Zhu

Pollard³

2017

Differentiation

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The human endometrium undergoes extensive morphological, biochemical and molecular changes under the influence of female sex steroid hormones. Besides the fact that estrogen stimulates endometrial cell proliferation and progesterone inhibits this proliferation and induces differentiation, there is limited knowledge about precise molecular mechanisms underlying human endometrial biology. The importance of paracrine signaling in endometrial physiology explains why in vitro culture of endometrial cells has been challenging. Researchers, therefore, have developed alternative experimental in vivo models for the study of endometrial biology. The objective of this review is to summarize the recent developments and work on these in vivo endometrial research models. The in vivo recombinant tissue models in which wild-type endometrial cells are combined with endometrial cells from a gene-targeted mouse strain followed by xenografting to host mice have been critical in confirming the significance of paracrine signaling between the epithelium and stroma in the growth regulation of the endometrium. Additionally, these studies have uncovered differences between the mouse and human, emphasizing the need for the development of experimental models specifically of the human endometrium. Recently, xenotransplants of human endometrial fragments into the subcutaneous space of host mice and endometrial xenografts of dissociated and recombined epithelial and stromal cells beneath the kidney capsule of immunodeficient host mice have proven to be highly promising tools for in vivo research of endometrial functions. For the first time, the latter approach provides an immense opportunity for the application of genome engineering, such as targeted ablation of endometrial genes for example by using CRISPR/CAS9 system. This research will begin to elucidate the functional role of specific genes in this complex tissue. Another advantage of xenotransplantation and xenograft models of the human endometrium is their use to investigate endometrial effects of new compounds and drugs without needing to give them to women. Underpinning the molecular mechanisms underlying endometrial functions is critical to ultimately advance our understanding of endometrial pathophysiology and develop targeted therapies to prevent and cure endometrial pathologies as well as enhance endometrial function when it is desired for fertility.

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“…During the WOI, the endometrium which has been primed by estrogen and progesterone is characterized by changes that are collectively termed endometrial receptivity (Lessey 2011). During the past decades, intensive morphological, histological, and molecular studies have defined the “signature” of the WOI in many species (Cheong et al 2013; Diaz-Gimeno et al 2014; Lessey 2011). …”

Section: Implantationmentioning

confidence: 99%

Implantation and Establishment of Pregnancy in Human and Nonhuman Primates

Fazleabas

2015

Advances in Anatomy, Embryology and Cell Biology

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Implantation and the establishment of pregnancy are critical for the propagation of the species, but yet remain the limiting steps in human and primate reproduction. Successful implantation requires a competent blastocyst and a receptive endometrium during a specific window of time during the menstrual cycle to initiate the bilateral communication required for the establishment of a successful pregnancy. This chapter provides an overview of these processes and discusses the molecular mechanisms associated with implantation of the blastocyst and decidualization of the uterus in primates.

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Transcriptomics of the human endometrium

Cited by 58 publications

References 100 publications

Human Endometrial Transcriptomics: Implications for Embryonic Implantation

Human Endometrial Transcriptomics: Implications for Embryonic Implantation

Xenografted tissue models for the study of human endometrial biology

Implantation and Establishment of Pregnancy in Human and Nonhuman Primates

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