Differences in dopamine- and noradrenaline-induced amylase release from the rat parotid gland

Sundström, Staffan; Carlsöö, Bengt; Danielsson, Åke; Henriksson, Roger

doi:10.1016/0014-2999(85)90396-6

Cited by 13 publications

(9 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is considered that salivary amylase is released due to direct activation of the β 1 -ARs in the acinar cells of the parotid glands (11). On the other hand, salivary amylase is induced by activation of the sympathetic nerves in the central nervous systems.…”

Section: Discussionmentioning

confidence: 99%

“…Salivary amylase is thought to reflect changes in noradrenaline levels due to increased activation of the sympathetic-adrenal-medullary system (9,10). It is conceivable that salivary amylase is released due to activation of β-adrenoceptors (ARs) in the acinar cells of the parotid glands (11) and/or the sympathetic nerves in the central nervous systems. However, the involvement of the sympathetic nerves or β 1 -and β 2 -ARs on the secretion of salivary amylase induced by various stimuli such as cisplatin, apomorphine, and LiCl has not yet been clarified.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Possible Involvement of β1 Receptors in Various Emetogen-Induced Increases in Salivary Amylase Activity in Rats

et al. 2011

View full text Add to dashboard Cite

Abstract. We investigated the inhibitory effects of β 1 -or β 2 -adrenoceptor (AR) antagonists on salivary amylase secretion produced by various emetic agents, such as cisplatin, apomorphine, and lithium chloride (LiCl), or the non-emetic agent β 1/2 -AR agonist isoprenaline in rats. We also determined the inhibitory effect of metoclopramide, a dopamine D 2 -receptor antagonist, on increases in the salivary amylase activity induced by apomorphine or granisetron, a 5-HT 3 -receptor antagonist, on LiCl-induced increased salivary amylase activity. Isoprenaline (0.01 mg/kg, s.c.) produced an increase in salivary amylase and the increase was inhibited by the β 1/2 -AR antagonist propranolol (5 mg/kg, s.c.) and β 1 -AR antagonist atenolol (2 mg/kg, s.c.) but not by the β 2 -AR antagonist butoxamine (8 mg/kg, s.c.). The increased amylase activity induced by cisplatin (15 mg/kg, i.v.), apomorphine (3 mg/kg, s.c.), or LiCl (120 mg/kg, i.p.) was inhibited significantly by atenolol (2 mg/kg, s.c.) but not by butoxamine (8 mg/kg, s.c.). In addition, increases in amylase activities induced by apomorphine and LiCl were inhibited significantly by metoclopramide (10 mg/kg, i.v.) and granisetron (3 mg/kg, i.v.), respectively. These results suggest that salivary amylase secretion induced by various emetogens is involved in β 1 -adrenoceptor activity and that salivary amylase activity is useful to detect emetogens with no direct β 1 -AR activation in rats, a species that does not exhibit vomiting.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Possible Involvement of β1 Receptors in Various Emetogen-Induced Increases in Salivary Amylase Activity in Rats

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Dopamine is an effective inducer of amylase release in rat parotid glands and the release is mediated by a Di-receptor (Sundstr6m et al 1985a). Adult sympathetic denervation induces supersensitivity in a variety of tissues including skeletal muscle, nerves (Fleming, 1981) and salivary glands (Emmelin, 1952;Emmelin & Engstrom, 1960;Stefano & Perec, 1981).…”

Section: Discussionmentioning

confidence: 99%

Dopamine actions in vitro on enzyme and electrolyte secretion from normal and sympathectomized rat parotid glands.

Danielsson

Henriksson

Sundström

et al. 1988

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. Adult rats were denervated unilaterally by removal of the left superior cervical ganglion or chemically denervated with 6-hydroxydopamine or reserpine. Two weeks later the parotid glands were used for in vitro secretory studies and their catecholamines and major metabolites were measured.2. Noradrenaline concentrations were reduced 2 weeks after surgical sympathectomy and reserpine pre-treatment 18 h previously, whereas 6-hydroxydopamine pre-treatment for 3 days reduced both noradrenaline and dopamine concentrations. 3. Dopamine caused a prominent amylase release from incubated control glands. However, a subsensitivity for dopamine-induced amylase release was recorded on the denervated side.4. Dopamine caused a prominent potassium efflux measured as 86Rb+ efflux from control glands, but was without effect in denervated glands. This is in contrast to noradrenaline-induced 86Rb+ efflux which was equally effective in both denervated and control glands.5. Dopamine caused [3H]noradrenaline efflux in control glands, but was without effect in surgically denervated glands and in glands pre-treated with reserpine or 6-hydroxydopamine.6. It is concluded that dopamine-induced potassium release is caused by a presynaptic action on noradrenergic nerves, whereas dopamine-induced amylase release has a presynaptic and a postsynaptic component. The results suggest a specific action of dopamine in salivary glands, with different effects on enzyme release and ionic fluxes.

show abstract

“…Data on the influence of dopamine on salivary secretion in mammals are sparse and often inconsistent. Earlier studies reported that dopamine and noradrenaline induce amylase secretion, as a result of dopamine being mainly mediated through postsynaptic D 1 -like receptors (30). The effect of the catecholamine was probably due to a minor extent to presynaptic interaction and activation of the presynaptic D 1 -like receptors, leading to noradrenaline release (30).…”

mentioning

confidence: 99%

“…Earlier studies reported that dopamine and noradrenaline induce amylase secretion, as a result of dopamine being mainly mediated through postsynaptic D 1 -like receptors (30). The effect of the catecholamine was probably due to a minor extent to presynaptic interaction and activation of the presynaptic D 1 -like receptors, leading to noradrenaline release (30). Other pharmacological and biochemical investigations reported that dopamine mediates amylase secretion indirectly through ACh and noradrenaline released from parasympathetic and sympathetic nerve terminals (12).…”

mentioning

confidence: 99%

Dopamine, vesicular transporters, and dopamine receptor expression in rat major salivary glands

Tomassoni

Traini

Mancini

et al. 2015

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

The localization of dopamine stores and the expression and localization of dopamine (DAT) and vesicular monoamine transporters (VMAT) type-1 and -2 and of dopamine D1-like and D2-like receptor subtypes were investigated in rat submandibular, sublingual, and parotid salivary glands by HPLC with electrochemical detection, as well as immunochemical and immunohistochemical techniques. Male Wistar rats of 2 mo of age were used. The highest dopamine levels were measured in the parotid gland, followed by the submandibular and sublingual glands. Western blot analysis revealed DAT, VMAT-1, VMAT-2, and dopamine receptors immunoreactivity in membrane preparations obtained from the three glands investigated. Immunostaining for dopamine and transporters was developed within striated ducts. Salivary glands processed for dopamine receptors immunohistochemistry developed an immunoreaction primarily in striated and excretory ducts. In the submandibular gland, acinar cells displayed strong immunoreactivity for the D2 receptor, while cells of the convoluted granular tubules were negative for both D1-like and D2-like receptors. Parotid glands acinar cells displayed the highest immunoreactivity for both D1 and D2 receptors compared with other salivary glands. The above localization of dopamine and dopaminergic markers investigated did not correspond closely with neuron-specific enolase (NSE) localization. This indicates that at least in part, catecholamine stores and dopaminergic markers are independent from glandular innervation. These findings suggest that rat major salivary glands express a dopaminergic system probably involved in salivary secretion. The stronger immunoreactivity for dopamine transporters and receptors in striated duct cells suggests that the dopaminergic system could regulate not only quality, but also volume and ionic concentration of saliva.

show abstract

Differences in dopamine- and noradrenaline-induced amylase release from the rat parotid gland

Cited by 13 publications

References 20 publications

Possible Involvement of β1 Receptors in Various Emetogen-Induced Increases in Salivary Amylase Activity in Rats

Possible Involvement of β1 Receptors in Various Emetogen-Induced Increases in Salivary Amylase Activity in Rats

Dopamine actions in vitro on enzyme and electrolyte secretion from normal and sympathectomized rat parotid glands.

Dopamine, vesicular transporters, and dopamine receptor expression in rat major salivary glands

Contact Info

Product

Resources

About