Differences in cell wall polysaccharide composition between embryogenic and non-embryogenic calli of Medicago arborea L.

Endress, Vanessa; Barriuso, Jorge; Rupérez, Pilar; Martín, Juan José Molina; Blázquez, Antonio; Villalobos, Nieves; Guerra, Hilario; Martı́n, Luisa

doi:10.1007/s11240-009-9531-0

Cited by 12 publications

(8 citation statements)

References 29 publications

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“…In the maize callus, the extracellular matrix surface network (ECMS) of the embryogenic cells contained the AGP epitope, which is recognised by the JIM4 antibody; while, the non-embryogenic callus cells were devoid of this epitope (Šamaj et al 1999, 2008). Analysis of the cell wall polysaccharide composition of the embryogenic and non-embryogenic calli that had been obtained from hypocotyl and petiole explants from Medicago arborea L. has revealed that the levels of total sugars, pectins, and hemicelluloses were higher in the embryogenic callus than in the non-embryogenic callus (Endress et al 2009). In addition, during the somatic embryogenesis of Trifolium nigrescens Viv., it was demonstrated that the low methylesterified homogalacturonan (HG), which is recognised by the JIM5 antibody, and the arabinan from side chains of rhamnogalacturonan I (RG I), which is recognised by the LM6 antibody, were detected in the embryogenic sectors of the explant (Pilarska et al 2013).…”

Section: Jim20mentioning

confidence: 99%

Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta

Popielarska-Konieczna

Sala

Abdullah

et al. 2020

Plant Cell Rep

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Key message Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit. Abstract The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue. In the presented study, this relationship was found in the callus tissue that had been differentiated from the isolated endosperm of the kiwiberry, Actinidia arguta. The experimental system was based on callus samples of exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway. The analyses which were performed using bright field, fluorescence and scanning electron microscopy techniques showed significant differences between the two types of calli. The organogenic tissue was compact and the outer walls of the peripheral cells were covered with granular structures. The non-organogenic tissue was composed of loosely attached cells, which were connected via a net-like structure. The extracellular matrices from both the non-and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus. Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).

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Section: Jim20mentioning

confidence: 99%

Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta

Popielarska-Konieczna

Sala

Abdullah

et al. 2020

Plant Cell Rep

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“…The use of monoclonal antibodies has shown that the distribution of different pectic epitopes in cell walls is developmentally regulated and can also be tissue, organ and species specific (Bush and McCann 1999;Willats et al 1999;McCartney et al 2000). In the embryogenic cultures of carrot (Kikuchi et al 1995) and Medicago arborea (Endress et al 2009) differences were evident in the pectic polysaccharide content between embryogenic and non-embryogenic callus cells. Data relating to the distribution of specific pectic epitopes during SE are scarce and indicate differences in the methyl-esterification of homogalacturonan (HG) during the early stages of SE (Verdeil et al 2001;Š amaj et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Arabinogalactan-protein and pectin epitopes in relation to an extracellular matrix surface network and somatic embryogenesis and callogenesis in Trifolium nigrescens Viv.

Pilarska

Knox

Konieczny

2013

Plant Cell Tiss Organ Cult

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The formation of an extracellular matrix surface network (ECMSN), and associated changes in the distribution of arabinogalactan-protein and pectin epitopes, have been studied during somatic embryogenesis (SE) and callogenesis of Trifolium nigrescens Viv. Scanning electron microscopy observations revealed the occurrence of an ECMSN on the surface of cotyledonary-staged somatic embryos as well as on the peripheral, non-regenerating callus cells. The occurrence of six AGP (JIM4, JIM8, JIM13, JIM16, LM2, MAC207) and four pectin (JIM5, JIM7, LM5, LM6) epitopes was analysed during early stages of SE, in cotyledonary-staged somatic embryos and in non-embryogenic callus using monoclonal antibodies. The JIM5 low methyl-esterified homogalacturonan (HG) epitope localized to ECMSN on the callus surface but none of the epitopes studied were found to localize to ECMSN over mature somatic embryos. The LM2 AGP epitope was detected during the development of somatic embryos and was also observed in the cell walls of meristematic cells from which SE was initiated. The pectic epitopes JIM5, JIM7, LM5 and LM6 were temporally regulated during SE. The LM6 arabinan epitope, carried by side chains of rhamnogalacturonan-I (RG-I), was detected predominantly in cells of embryogenic swellings, whilst the LM5 galactan epitope of RG-I was uniformly distributed throughout the ground tissue of cotyledonary-staged embryoids but not detected at the early stages of SE. Differences in the distribution patterns of low and high methyl-esterified HG were detected: low ester HG (JIM5 epitope) was most abundant during the early steps of embryo formation and highly methyl-esterified form of HG (JIM7 epitope) became prevalent during embryoid maturation.

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“…Pectins are the major components of the primary cell walls of all terrestrial plants (Pilarska et al, 2013). In studies with Medicago arborea (Endress et al, 2009), pectin content diff erences were observed in embryogenic and non-embryogenic callus cells. Evidence regarding the specifi c distribution of pectin epitopes during somatic embryogenesis is scarce (Pilarska et al, 2013), but embryogenic callusproliferative cells have been shown to contain large amounts of reactive pectins (Xu et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

AUXIN PULSE IN THE INDUCTION OF SOMATIC EMBRYOS OF Eucalyptus

et al. 2019

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The objective of this study was to evaluate the effect of auxin pulse intervals on the induction of somatic embryos of Eucalyptus grandis x E. urophylla and to describe the embryogenic behavior of callus under the effect of auxinic stress. Cotyledons were inoculated in culture medium containing 207.07 µM picloram, a treatment considered as auxin pulse. Explants that were in the auxin pulse treatment were transferred to semisolid or liquid medium containing 20.71 µM picloram after one, two, four or eight days of auxin pulse. In a second experiment, explants that were on auxin pulse treatment were transferred to semi-solid medium containing 20.71 µM picloram after one, two or three days of auxin pulse. Auxiliary picloram pulse treatments (207.02 µM) can be used as an initial source of stress for the acquisition of embryogenic competence. The oxidation of cotyledonary explants may be considered as an indication of the formation of embryogenic calli. The presence of pectins in peripheral regions of somatic pro-embryos can be considered as a marker of somatic embryogenesis in cotyledonary explants of Eucalyptus grandis x E. urophylla.

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Differences in cell wall polysaccharide composition between embryogenic and non-embryogenic calli of Medicago arborea L.

Cited by 12 publications

References 29 publications

Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta

Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta

Arabinogalactan-protein and pectin epitopes in relation to an extracellular matrix surface network and somatic embryogenesis and callogenesis in Trifolium nigrescens Viv.

AUXIN PULSE IN THE INDUCTION OF SOMATIC EMBRYOS OF Eucalyptus

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