Differences in bovine placentome blood vessel density and transcriptomics in a mid to late-gestating maternal nutrient restriction model

Reid, Dana; Burnett, D. D.; Contreras-Correa, Zully E; Lemley, Caleb O

doi:10.1016/j.placenta.2021.12.004

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…Prior to the present study, the effects of melatonin on placental nutrient transporter expression in a compromised pregnancy has not been reported, however, in a sheep study by Edwards, McKnight, Askelson, McKnight, Dunlap and Satterfield [6], SLC38A2 abundance was reduced in placentomes from nutrient restricted IUGR pregnancies, which is contradictory to the current study. Previous work from our lab found genes associated with peptide biosynthesis and peptide metabolism were upregulated in placentomes from nutrient restricted heifers [29]. In early gestation nutrient restricted heifers, neutral amino acid transporters were reduced in uteroplacental tissues [7].…”

Section: Discussionmentioning

confidence: 73%

Melatonin Supplementation Alters Maternal and Fetal Amino Acid Concentrations and Placental Nutrient Transporters in a Nutrient Restriction Bovine Model

et al. 2022

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Melatonin rescues uterine blood flow and fetal body weight in a seasonal dependent manner within a nutrient restriction bovine model. We sought to identify the effects of nutrient restriction, melatonin, and sampling time on maternal and fetal amino acids, and placental nutrient transporters. Pregnant heifers received adequate or restricted nutrition, and 20 mg of melatonin or placebo from gestational days 160–240 over two seasons. On day 240 maternal and fetal blood, amnion, and placentomes were collected. Amino acid concentrations were determined by gas chromatography-mass spectrometry. Caruncle and cotyledon tissues were assessed for nutrient transporter density by qPCR. Data were analyzed using the MIXED procedure of SAS for fixed effects. In fall, melatonin rescued effects of nutrient restriction on System N, Anion, and total maternal amino acids. Furthermore, melatonin rescued effects of nutrient restriction on Systems A, N, Br, Bo, and essential amnion amino acids. In summer, melatonin rescued effects of nutrient restriction in Systems Br and Bo maternal amino acids. Furthermore, melatonin rescued effects of nutrient restriction on caruncle SLC38A10 and SLC38A2. Melatonin rescued effects of nutrient restriction in a seasonal dependent manner. These data align with previous studies suggesting melatonin is a more effective therapeutic in summer months.

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Section: Discussionmentioning

confidence: 73%

Melatonin Supplementation Alters Maternal and Fetal Amino Acid Concentrations and Placental Nutrient Transporters in a Nutrient Restriction Bovine Model

et al. 2022

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“…RNA sequencing showed the differences in gene expression pattern between the LW and MW placentas and the multiple genes involved in the ribosomal pathway and mTOR signaling pathway in the LW placentas. Any disturbance in the ribosome environment could have devastating effects on placental development (Janssen et al, 2015; Reid et al, 2022). Ribosomal proteins (RPs) are essential in regulating translation for facilitating placental growth and development (Kondrashov et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

RACK1 may participate in placental development at mid‐gestation via regulating trophoblast cell proliferation and migration in pigs

Gong

et al. 2023

Molecular Reproduction Devel

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Intrauterine growth restriction (IUGR) is a severe complication in swine production.Placental insufficiency is responsible for inadequate fetal growth, but the specific etiology of placental dysfunction-induced IUGR in pigs remains poorly understood.In this work, placenta samples supplying the lightest weight (LW) and mean weight (MW) pig fetuses in the litter at Day 65 (D65) of gestation were collected, and the relationship between fetal growth and placental morphologies and functions was investigated using histomorphological analysis, RNA sequencing, quantitative polymerase chain reaction, and in vitro experiment in LW and MW placentas.Results showed that the folded structure of the epithelial bilayer of LW placentas followed a poor and incomplete development compared with that of MW placentas.A total of 654 differentially expressed genes (DEGs) were screened out between the LW and MW placentas, and the gene encodes receptor for activated C kinase 1 (RACK1) was found to be downregulated in LW placentas. The DEGs were mainly enriched in translation, ribosome, protein synthesis, and mammalian target of rapamycin (mTOR) signaling pathway according to gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. In vitro experiments indicated that the decreased RACK1 in LW placentas may be involved in abnormal development of placental folds (PFs) by inhibiting the proliferation and migration of porcine trophoblast cells. Taken together, these results revealed that RACK1 may be a vital regulator in the development of PFs via regulating trophoblast cell proliferation and migration in pigs.

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“…RNA sequencing showed the differences in gene expression pattern between the LW and MW placentas and the multiple genes involved in the ribosomal pathway and mTOR signaling pathway in the LW placentas. Any disturbance in the ribosome environment could have devastating effects on placental development [31, 32]. Ribosomal proteins (RPs) are essential in regulating translation for facilitating placental growth and development [33].…”

Section: Discussionmentioning

confidence: 99%

RACK1 may participate in placental development via regulating proliferation and migration of trophoblast cell in pigs following intrauterine growth restriction

Wu¹,

Hu²,

Gong³

et al. 2022

Preprint

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Intrauterine growth restriction (IUGR) is a severe complication in swine production. Placental insufficiency is responsible for inadequate fetal growth, but the specific etiology of placental dysfunction-induced IUGR in pigs remains poorly understood. In this work, placenta samples supplying the lightest-weight (LW) and mean-weight (MW) pig fetuses in the litter at day 65 (D65) of gestation were collected, and the relationship between fetal growth and placental morphologies and functions was investigated using histomorphological analysis, RNA sequencing, quantitative polymerase chain reaction, and in-vitro experiment in LW and MW placentas. Results showed that the folded structure of the epithelial bilayer of LW placentas followed a poor and incomplete development compared with that of MW placentas. A total of 632 differentially expressed genes (DEGs) were screened out between the LW and MW placentas, and RACK1 was found to be downregulated in LW placentas. The DEGs were mainly enriched in translation, ribosome, protein synthesis, and mTOR signaling pathway according to GO and KEGG enrichment analyses. In-vitro experiments indicated that the decreased RACK1 in LW placentas may be involved in abnormal development of placental folds (PFs) by inhibiting the proliferation and migration of porcine trophoblast cells. Taken together, these results revealed that RACK1 may be a vital regulator in the development of PFs via regulating trophoblast ribosome function, proliferation, and migration in pigs.

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Differences in bovine placentome blood vessel density and transcriptomics in a mid to late-gestating maternal nutrient restriction model

Cited by 7 publications

References 25 publications

Melatonin Supplementation Alters Maternal and Fetal Amino Acid Concentrations and Placental Nutrient Transporters in a Nutrient Restriction Bovine Model

Melatonin Supplementation Alters Maternal and Fetal Amino Acid Concentrations and Placental Nutrient Transporters in a Nutrient Restriction Bovine Model

RACK1 may participate in placental development at mid‐gestation via regulating trophoblast cell proliferation and migration in pigs

RACK1 may participate in placental development via regulating proliferation and migration of trophoblast cell in pigs following intrauterine growth restriction

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