Differences in bacterial diversity of host-associated populations of Phylloxera notabilis Pergande (Hemiptera: Phylloxeridae) in pecan and water hickory

Medina, Raúl F.; Nachappa, Punya; Tamborindeguy, Cécilia

doi:10.1111/j.1420-9101.2010.02215.x

Cited by 48 publications

(43 citation statements)

References 84 publications

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“…Erwinia, Pantoea, Enterobacter, Serratia, Escherichia and Pseudomonas have been identified internally from thrips (de Vries et al 2001a; Gitaitis et al 2003; Chanbusarakum & Ullman 2008; de Vries et al 2008; de Vries et al 2012). Erwinia herbicola and Enterobacter agglomerans have been synonymized with Pantoea agglomerans so these 3 genera identified in chilli thrips could represent a single taxon found in association with several insects (Medina et al 2011). Agrobacterium, Methylobacterium, Pseudomonas and Escherichia have been isolated from the exoskeleton of Sericothrips staphylinus (Yamoah et al 2008).…”

Section: Resultsmentioning

confidence: 99%

Estimating Bacterial Diversity inScirtothrips dorsalis(Thysanoptera: Thripidae) via Next Generation Sequencing

Dickey¹,

Trease²,

Jara-Cavieres³

et al. 2014

Florida Entomologist

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The last 2 decades have produced a better understanding of insect-microbial associations and yielded some important opportunities for insect control. However, most of our knowledge comes from model systems. Thrips (Thysanoptera: Thripidae) have been understudied despite their global importance as invasive species, plant pests and disease vectors. Using a culture and primer independent next-generation sequencing and metagenomics pipeline, we surveyed the bacteria of the globally important pest, Scirtothrips dorsalis Hood. The most abundant bacterial phyla identified were Actinobacteria and Proteobacteria and the most abundant genera were Propionibacterium, Stenotrophomonas, and Pseudomonas. A total of 189 genera of bacteria were identified. The absence of any vertically transferred symbiont taxa commonly found in insects is consistent with other studies suggesting that thrips primarilly acquire resident microbes from their environment. This does not preclude a possible beneficial/intimate association between S. dorsalis and the dominant taxa identified and future work should determine the nature of these associations.

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Section: Resultsmentioning

confidence: 99%

Estimating Bacterial Diversity inScirtothrips dorsalis(Thysanoptera: Thripidae) via Next Generation Sequencing

Dickey¹,

Trease²,

Jara-Cavieres³

et al. 2014

Florida Entomologist

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“…Either scenario would link this clade to nutrient acquisition strategies. Although phylloxerids lack stable nutritional endosymbionts, numerous microbial partners exist within the gut microbiome (PDN unpublished data, [20]). How gut microbial community dynamics modify sap-feeder fitness is less well understood.…”

Section: Discussionmentioning

confidence: 99%

“…Insects within the Phylloxeridae are considered sister to the aphids (Aphidoidea: Aphididae) and adelgids (Phylloxeroidea: Adelgidae), groups that also retain galling and free-living species [18]. In contrast with aphids and adelgids that harbor symbionts in bacteriocytes, Phylloxeridae species lack stable intracellular symbionts [19, 20]. Further, Phylloxeridae comprises numerous life history strategies, including galling and free-living species that allow a phylogenetically controlled comparison to understand how these strategies arose with respect to their nutrient acquisition and metabolism.…”

Section: Introductionmentioning

confidence: 99%

Plant manipulation through gall formation constrains amino acid transporter evolution in sap-feeding insects

Zhao

Nabity

2017

BMC Evol Biol

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BackgroundThe herbivore lifestyle leads to encounters with plant toxins and requires mechanisms to overcome suboptimal nutrient availability in plant tissues. Although the evolution of bacterial endosymbiosis alleviated many of these challenges, the ability to manipulate plant nutrient status has evolved in lineages with and without nutritional symbionts. Whether and how these alternative nutrient acquisition strategies interact or constrain insect evolution is unknown. We studied the transcriptomes of galling and free-living aphidomorphs to characterize how amino acid transporter evolution is influenced by the ability to manipulate plant resource availability.ResultsUsing a comparative approach we found phylloxerids retain nearly all amino acid transporters as other aphidomorphs, despite loss of nutritional endosymbiosis. Free living species show more transporters than galling species within the same genus, family, or infraorder, indicating plant hosts influence the maintenance and evolution of nutrient transport within herbivores. Transcript profiles also show lineage specificity and suggest some genes may facilitate life without endosymbionts or the galling lifestyle.ConclusionsThe transcript abundance profiles we document across fluid feeding herbivores support plant host constraint on insect amino acid transporter evolution. Given amino acid uptake, transport, and catabolism underlie the success of herbivory as a life history strategy, this suggests that plant host nutrient quality, whether constitutive or induced, alters the selective environment surrounding the evolution and maintenance of endosymbiosis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12862-017-1000-5) contains supplementary material, which is available to authorized users.

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“…Prior to DNA extraction, spiders were surface sterilized with sequential 10 s rinses of ethanol, deionized water, 5% bleach solution and deionized water (adapted from Medina et al, 2011). DNA was extracted using a Chelex extraction protocol adapted from White et al (2009): abdomens of adults were pulverized in 4 μl of Proteinase K, incubated in a 20% Chelex bead solution for 60 min at 37°C and then heat shocked for 10 min at 95°C to deactivate Proteinase K. Extraction quality was assessed by measuring DNA concentration using a Nanodrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and by PCR using universal eukaryotic primers NSF 18/19 or COI (Supplementary Table S1).…”

Section: Establishment Of Laboratory Matrilines and Determination Of mentioning

confidence: 99%

Multiple endosymbiont infections and reproductive manipulations in a linyphiid spider population

et al. 2015

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In many arthropods, maternally inherited endosymbiotic bacteria can increase infection frequency by manipulating host reproduction. Multiple infections of different bacteria in a single host population are common, yet few studies have documented concurrent endosymbiont phenotypes or explored their potential interactions. We hypothesized that spiders might be a particularly useful taxon for investigating endosymbiont interactions, because they are host to a plethora of endosymbiotic bacteria and frequently exhibit multiple infections. We established two matrilines from the same population of the linyphiid spider Mermessus fradeorum and then used antibiotic curing and controlled mating assays to demonstrate that each matriline was subject to a distinct endosymbiotic reproductive manipulation. One matriline was co-infected with Rickettsia and Wolbachia and produced offspring with a radical female bias. Antibiotic treatment eliminated both endosymbionts and restored an even sex ratio to subsequent generations. Chromosomal and fecundity observations suggest a feminization mechanism. In the other matriline, a separate factorial mating assay of cured and infected spiders demonstrated strong cytoplasmic incompatibility (CI) induced by a different strain of Wolbachia. However, males with this Wolbachia induced only mild CI when mated with the Rickettsia-Wolbachia females. In a subsequent survey of a field population of M. fradeorum, we detected these same three endosymbionts infecting 55% of the spiders in almost all possible combinations, with nearly half of the infected spiders exhibiting multiple infection. Our results suggest that a dynamic network of endosymbionts may interact both within multiply infected hosts and within a population subject to multiple strong reproductive manipulations.

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Differences in bacterial diversity of host-associated populations of Phylloxera notabilis Pergande (Hemiptera: Phylloxeridae) in pecan and water hickory

Cited by 48 publications

References 84 publications

Estimating Bacterial Diversity inScirtothrips dorsalis(Thysanoptera: Thripidae) via Next Generation Sequencing

Estimating Bacterial Diversity inScirtothrips dorsalis(Thysanoptera: Thripidae) via Next Generation Sequencing

Plant manipulation through gall formation constrains amino acid transporter evolution in sap-feeding insects

Multiple endosymbiont infections and reproductive manipulations in a linyphiid spider population

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