Difference of concentration of placental soluble fms-like tyrosine kinase-1(sFlt-1), placental growth factor (PlGF), and sFlt-1/PlGF ratio in severe preeclampsia and normal pregnancy

Gurnadi, Jeffry Iman; Mose, Johannes C.; Handono, Budi; Satari, Mieke Hemiawati; Anwar, Anita Deborah; Fauziah, Prima Nanda; Pramatirta, Akhmad Yogi; Rihibiha, Dwi Davidson

doi:10.1186/s13104-015-1506-0

Cited by 15 publications

(12 citation statements)

References 21 publications

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“…In a study by Levine et al, a higher mean sFlt-1 value of 4382 pg/ml was reported in women with preeclampsia compared to value of 1643 pg/ml in the control group [18]. In the two separate studies by Kim et al [19] and Gurnadi JI et al [20], elevated serum concentrations of sFlt-1 in women with preeclampsia was found.…”

Section: Discussionmentioning

confidence: 99%

A cross sectional study to assess the sFlt-1:PlGF ratio in pregnant women with and without preeclampsia

Pant

Yadav

Sharma

2019

BMC Pregnancy Childbirth

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Background Preeclampsia is a multisystem disorder characterized by vascular endothelial malfunction occurring after 20 weeks of gestation. Placental soluble fms-like tyrosine kinase-1 (sFlt-1) is an antiangiogenic factor and placental growth factor (PlGF) is a potent angiogenic factor. The imbalance between these factors during placenta and fetal development has been shown to play a role in endothelial damage in preeclampsia. Preeclampsia is the leading cause of maternal mortality in Nepal. This study was designed to compare the sFlt1:PLGF ratio in pregnant women with and without preeclampsia attending Tribhuvan University Teaching Hospital (TUTH). Method An observational cross-sectional study was performed in the Gynecology and Obstetrics Department of TUTH involving forty-four subjects with preeclampsia and forty-four age- and gestational-week-matched normal pregnant subjects as controls. Blood pressure, urinary protein levels, serum sFlt-1 levels, serum PlGF levels and the sFlt-1:PlGF ratio was compared in both the cases and control. The concentrations of sFlt-1 and PlGF were measured with commercially available ELISA kits. SPSS ver. 20.0 was used to analyze the data. Results There was no significant difference in age or gestational age in either study group. The ratio of the sFlt-1 and PlGF concentrations was significantly higher in women with preeclampsia (31.6 ± 9.6) than in the controls (3.2 ± 1.3). Likewise, diastolic blood pressure was significantly associated ( p -value 0.000), whereas the severity of proteinuria was not associated (p-value 0.773) with the sFlt-1:PlGF ratio in women with preeclampsia. The significantly higher ratio (35.51 ± 8.1 versus 25.4 ± 8.7) was found in women with preeclampsia who developed complications than the group of women with preeclampsia who did not develop complication. Conclusion The sFlt-1:PlGF ratio is significantly higher in Nepalese women with preeclampsia than in normal controls and this finding can be applied for further planned clinical trials.

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Section: Discussionmentioning

confidence: 99%

A cross sectional study to assess the sFlt-1:PlGF ratio in pregnant women with and without preeclampsia

Pant

Yadav

Sharma

2019

BMC Pregnancy Childbirth

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“…The alterations of sFlt‐1 and PIGF expression are involved in PE pathological process (Gurnadi et al, ; Lehnen et al, ). PIGF, a key local mediator in angiogenesis, belongs to the family of VEGF and mainly expressed by placentas (Gurnadi et al, ; Lehnen et al, ). Previous reports demonstrate that change in the circulating level of PIGF is closely associated with PE occurrence (Dechend & Luft, ; Grill et al, ).…”

Section: Discussionmentioning

confidence: 99%

Tetramethylpyrazine reduces the consequences of nitric oxide inhibition in pregnant rats

Shen

Zhou

et al. 2019

Journal Cellular Physiology

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Pre-eclampsia (PE) is closely associated with perinatal morbidity and mortality and we want to investigate tetramethylpyrazine (TMP)'s effects on PE. Pregnant Sprague-Dawley rats were randomly divided into five groups: normal pregnant (PC), PE, PE+TMP 20 mg/kg, PE+TMP 40 mg/kg, and PE+TMP 60 mg/kg group. The PE rat model was established via L-NAME treatment. Systolic blood pressures (SBP) and urinary protein concentration were detected via the tail-cuff method and CBB kit, respectively. mRNA levels of key genes were analyzed via quantitative PCR and protein levels of key genes were measured by ELISA or western blot. TMP decreased SBP and urinary protein concentration of PE rats. TMP inhibited L-NAME-induced decrease in pups alive ratio, pups weight, and the ratio of pups/placenta weight and reversed L-NAME induced changes in placental histology, whereas it had little effect on placental weight. Urinary nephrin and podocin expressions were enhanced and serum placental growth factor level was decreased in PE rats, whereas TMP inhibited the above phenomena. TMP suppressed L-NAME-induced sFlt-1 upregulation in serums and kidneys of PE rats, whereas it downregulated IL-6 and MCP-1 expression in PE rats' serums, placentas and kidneys. TMP also suppressed the increase in placental sFlt-1 and vascular endothelial growth factor level caused by L-NAME. In addition, TMP inhibited CHOP and GRP78 expressions and decreased the ratio of p-elF2α/elF2α in PE rats. TMP attenuated the consequences of NO inhibition in pregnant rats. K E Y W O R D SER stress, hypertension, pre-eclampsia, proteinuria, tetramethylpyrazine

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“…The connection between circulating sFlt-1 and PlGF in preeclampsia was explored by previous studies suggesting that upregulation of the anti-angiogenic sFlt-1 in pre-eclampsia leads to binding of the angiogenic factor PlGF, with a consequent decrease in circulating free PlGF levels [6,16]. to brain edema.…”

Section: Discussionmentioning

confidence: 99%

“…to brain edema. This aberrant angiogenesis and hypertension are the hallmarks of pre-eclampsia [16].…”

Section: Discussionmentioning

confidence: 99%

PlGF, sFlt-1 and sFlt-1/PlGF ratio: Promising markers for predicting pre-eclampsia

Marwa¹

2018

J Gynecol Res Obstet

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Pre-eclampsia is a hypertensive disorder complicating 5-8% of pregnancies [1]. It is characterized by hypertension and proteinuria and is a leading cause of maternal mortality in 15-20% of pregnant women in developed countries versus 40-80% in developing countries [2,3]. Pre-eclampsia is a state of angiogenic imbalance associated with endothelial dysfunction within specifi c vascular beds (4). The abnormalities in the development of the placental vasculature could occur early, weeks to months before the development of the clinical manifestations of pre-eclampsia [5]. Placental growth factor (PlGF), a member of the vascular endothelial growth factor (VEGF) family, is one of the main factors that play a key role in the remodeling process of maternal arteries in normal pregnancy. This growth factor is released into the bloodstream by the migrating trophoblasts [6]. The soluble fms-like tyrosine kinase-1 (sFlt-1), also known as soluble VEGF receptor-1, is a variant of the VEGF receptor, which is secreted from endothelial cells, monocytes, and placenta. The sFlt-1 is capable of binding with both VEGF and PlGF, consequently regulating their action [7]. Alterations in maternal serum concentrations of PlGF and sFlt-1 have been reported to precede the onset of clinically identifi able preeclampsia as early as the fi rst trimester [6]. The aim of our study is to assess the clinical utility of PlGF and sFlt-1 as early predictors of preeclampsia.

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Difference of concentration of placental soluble fms-like tyrosine kinase-1(sFlt-1), placental growth factor (PlGF), and sFlt-1/PlGF ratio in severe preeclampsia and normal pregnancy

Cited by 15 publications

References 21 publications

A cross sectional study to assess the sFlt-1:PlGF ratio in pregnant women with and without preeclampsia

A cross sectional study to assess the sFlt-1:PlGF ratio in pregnant women with and without preeclampsia

Tetramethylpyrazine reduces the consequences of nitric oxide inhibition in pregnant rats

PlGF, sFlt-1 and sFlt-1/PlGF ratio: Promising markers for predicting pre-eclampsia

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