Difference in virulence and composition of a cariogenic biofilm according to substratum direction

Dang, Minh-Huy; Jung, Jieun; Choi, Hyeon-Mi; Jeon, Jongho

doi:10.1038/s41598-018-24626-2

Cited by 10 publications

(7 citation statements)

References 24 publications

(25 reference statements)

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“…In addition to the production of acids (acidogenicity), other virulence properties associated with cariogenic bacteria are their abilities of synthesizing more acids in a low‐pH medium (aciduricity), being resistant to this pH level without restricting their cellular functions (acidophilicity), and producing glucans and fructans, which promote their adherence to the dental surface and serve as a nutrient reserve (Dang et al, 2018 ; Kuramitsu & Wang, 2006 ). These properties adversely influence the prevalence and incidence of caries by hindering the oral immune response and the action of saliva as a buffer system (Rosier et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Does high sugar intake really alter the oral microbiota?: A systematic review

Díaz

Fong

Bedoya-Correa

et al. 2022

Clinical & Exp Dental Res

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Objectives: Diet is one of the main factors influencing the diversity and interactions of the oral microbiota. The purpose of this study is to determine the impact of sugar intake on the microbial diversity and bacteria that predominate under these conditions.Material and Methods: A systematic review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guide, using the PubMed, Scopus, and Science Direct databases and combinations of the words "microbiota," "microbiology," "bacteria," "sugars," "dysbiosis," "caries," "microbiome," "oral microbial," and "oral microbiota profile pattern." The selection criteria included year, language, type of publication, comparison of microbiota during low and high sugar intake, and bacterial identification by molecular sequencing of the 16S subunit of ribosomal RNA.Results: Out of a total of 374 papers that came up after the initial search, 8 met the criteria for this review. The papers included research on populations comprising children, young adults, and adults, with most of the studies reporting selection criteria for the participants and using validated instruments to determine sugar intake. Apart from one study, all others reported for high sugar intake conditions a significant decrease in microbial diversity of the oral microbiome and the predominance of several bacterial genera or species, including Streptococcus, Scardovia, Veillonella, Rothia, Actinomyces, and Lactobacillus.Conclusions: Sugar-rich diets have a significantly unfavorable effect on the diversity and balance of oral microbiota; however, further studies are required to determine the exact role of sugar in microbial interactions.

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Section: Introductionmentioning

confidence: 99%

Does high sugar intake really alter the oral microbiota?: A systematic review

Díaz

Fong

Bedoya-Correa

et al. 2022

Clinical & Exp Dental Res

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“…Although secondary caries is affected by numerous factors, one of the important factors is the local chemical environment, such as acid production, which is the main virulence factor of S. mutans that leads to the demineralization. The concentration of H + production is reportedly related to the number of viable acidogenic cells in the biofilm (Dang et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Immobilizing Bactericides on Dental Resins via Electron Beam Irradiation

et al. 2021

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Polymerizable bactericides, such as quaternary ammonium compound–based monomers, have been intensively studied as candidates for immobilizing antibacterial components on dental resin. However, they predominantly exhibit a bacteriostatic behavior, rather than bactericidal, as the immobilized components are left with insufficient molecular movement to disrupt the bacterial surface structure through contact-mediated action. In this study, we developed a novel strategy to increase the density of the immobilized bactericide and enhance its antibacterial/antibiofilm properties by combining a surface-grafting technique with electron beam irradiation. A solution of the quaternary ammonium compound–based monomer, 12-methacryloyloxydodecylpyridinium bromide (MDPB), was coated on polymethyl methacrylate (PMMA) resin specimens at the concentrations of 30, 50, and 80 wt%. The coated resins were subsequently exposed to 10 MeV of electron beam irradiation at 50 and 100 kGy, followed by thermal stabilization at 60 °C. The antibacterial effect was evaluated by inoculating a Streptococcus mutans suspension on the coated PMMA resin samples, which exhibited bactericidal effects even after 28 d of aging ( P < 0.05, Tukey’s honestly significant difference test). Transmission electron microscopy and bacteriolytic activity evaluation revealed that the S. mutans cells had sustained membrane depolarization. Furthermore, the antibiofilm effects against S. mutans and bacteria collected from human saliva were assessed. The thickness and the percentage of membrane-intact cells of the S. mutans and multispecies biofilms formed on the MDPB-immobilized surfaces were significantly lower than the uncoated PMMA specimens, even after 28-d aging ( P < 0.05, Tukey’s honestly significant difference test). Thus, the immobilization of antibacterial MDPB via electron beam irradiation induced rapid membrane depolarization, increasing membrane permeability and eventually causing cell death. Our strategy substantially enhances the antibacterial properties of the resinous materials and inhibits biofilm formation, therefore demonstrating significant potential for preventing infectious diseases in the oral environment.

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“…mutans biofilms were calculated using the dry weight, which was derived from the biochemical study above, and the total biovolume of the biofilms (live cells + dead cells + EPSs), which was derived from the CLSM study above. The biofilm density (μg/μm 3 ) is defined as the dry weight (μg/μm 2 ) divided by the total biovolume of the biofilms (μm 3 /μm 2 ) [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of cigarette smoking on the growth of Streptococcus mutans biofilms: An in vitro study

Han

2021

PLoS ONE

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The increased incidence of dental caries by cigarette smoking (CS) has been widely reported in epidemiological studies, but the relationship between CS and cariogenic biofilm growth has been rarely studied. This study aims to investigate the effects of CS exposure on the growth and virulence of Streptococcus mutans biofilms (S. mutans). Briefly, S. mutans biofilms were formed on saliva-coated hydroxyapatite disks, which were exposed to CS 1, 3, and 6 times per day, respectively. In addition, S. mutans biofilms without CS exposure were considered as the control group. Acidogenicity, dry weight, colony-forming units (CFUs), water-soluble/insoluble extracellular polysaccharides (EPSs), and intracellular polysaccharides (IPSs) were analyzed and confocal laser scanning microscopy (CLSM) images of 74-h-old S. mutans biofilms were obtained. The lowest accumulation of biofilms and EPSs were detected in the 6 times/day CS exposure group compared with those of the control group and other CS exposure groups in 74-h-old S. mutans biofilms. CLSM also revealed the lowest bacterial count (live and dead cells) and EPSs biovolume in the six times/day CS exposure group in 74-h-old S. mutans biofilms. CS exposure inhibited the growth of S. mutans biofilm in vitro study, the anti-cariogenic biofilm formation was enhanced with a dose (frequency)-dependent at which frequency has more influence in the present findings.

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Difference in virulence and composition of a cariogenic biofilm according to substratum direction

Cited by 10 publications

References 24 publications

Does high sugar intake really alter the oral microbiota?: A systematic review

Does high sugar intake really alter the oral microbiota?: A systematic review

Immobilizing Bactericides on Dental Resins via Electron Beam Irradiation

Effects of cigarette smoking on the growth of Streptococcus mutans biofilms: An in vitro study

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