Difference in reactions of apricot and peach cultivars to Plum pox virus: serological and symptomatological evaluation

Polák, J.; Oukropec, I.; Krška, B.; Pívalová, J.; Miller, William M.

doi:10.17221/3873-hortsci

Cited by 8 publications

(5 citation statements)

References 11 publications

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“…Leathery leaves with thickened veins get yellow during June and drop off. That is why no symptoms can be observed by the end of June especially in cultivars more resistant to PPV (Polák et al 2003). In more susceptible peach cultivars PPV symptoms develop in the third, contingently in the fourth and further leaves of growing branch.…”

Section: Methodsmentioning

confidence: 99%

Investigation on the incidence of Plum pox virus in fruit nurseries of the Czech Republic

Polák¹,

Komínek²

2016

Plant Prot. Sci.

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Polák J., Komínek P. (2016): Investigation on the incidence of Plum pox virus in fruit nurseries of the Czech Republic. Plant Protect. Sci., 52: 158-163.Nine different visual evaluations of Plum pox virus (PPV) presence were carried out in four nurseries during 2012-2015. Results of visual evaluation were verified by ELISA. The presence of PPV was confirmed by ELISA in all the trees showing PPV symptoms. PPV has never been detected by ELISA in trees without PPV symptoms on leaves. A very low occurrence of PPV was proved in plums in all the four nurseries. There were two cases of higher occurrence of PPV in plum cultivars, where PPV originated from infected grafts. The PPV occurrence was 0.126 and 1.59%. The average occurrence of PPV in plums was 0.075%, 93 trees out of 123 630 inspected were PPV-infected. All the PPVinfected trees were destroyed immediately. No new PPV infection was proved later in season (August-September). In comparison with the situation in the 60s of the last century, the PPV occurrence in plums was 2.48% in one nursery in 1963 and there were nurseries with PPV occurrence exceeding 5%. All growing plants were destroyed in this case. The occurrence of PPV in the Czech fruit nurseries today is more than hundred times lower in comparison with the situation fifty years ago. The incidental occurrence of PPV in nurseries cannot be excluded in countries and areas with the endemic presence of PPV (in the Czech Republic and in most European countries). Visual inspection of PPV symptoms in nurseries confirmed by ELISA testing is sufficient. Infected plants must be removed immediately.

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Section: Methodsmentioning

confidence: 99%

Investigation on the incidence of Plum pox virus in fruit nurseries of the Czech Republic

Polák¹,

Komínek²

2016

Plant Prot. Sci.

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“…Among the 85 accessions used for GWA analysis, 46 were evaluated for PPV-M susceptibility (Additional file 1 ). The phenotypes of the remaining accessions were derived from studies adopting the same viral strain and evaluation protocol [ 59 – 61 ], except for a few cases of highly susceptible accessions derived from other publications [ 7 , 8 , 62 ]. Seedlings from three pseudo BC1 populations derived from ‘Orion’ (peach) x SD45, 75 and 81 (thress ‘Summergrand’ peach x P. davidiana ‘P1908’ selections) and composed of 39, 18 and 13 individuals, respectively, were evaluated using the same protocol described above.…”

Section: Methodsmentioning

confidence: 99%

“…The introgression of resistance through hybrid selections has been unsuccessful so far due to several drawbacks, including the lack of resistance-associated molecular markers. The vast majority of peach accessions are highly susceptible to the PPV-M strain, although genotypes showing lesser symptom severity upon infection have been reported [ 7 , 8 ]. Most, if not all, the peach varieties are symptomless when infected by PPV-D strain [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic dissection of Sharka disease tolerance in peach (P. persica L. Batsch)

et al. 2017

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Background Plum pox virus (PPV), agent of Sharka disease, is the most important quarantine pathogen of peach (P. persica L. Batsch). Extensive evaluation of peach germplasm has highlighted the lack of resistant sources, while suggesting the presence of a quantitative disease resistance, expressed as reduction in the intensity of symptoms. Unravelling the genetic architecture of peach response to PPV infection is essential for pyramiding resistant genes and for developing more tolerant varieties. For this purpose, a genome-wide association (GWA) approach was applied in a panel of accessions phenotyped for virus susceptibility and genotyped with the IPSC peach 9 K SNP Array, and coupled with an high-coverage resequencing of the tolerant accession ‘Kamarat’.ResultsGenome-wide association identified three highly significant associated loci on chromosome 2 and 3, accounting for most of the reduction in PPV-M susceptibility within the analysed peach population. The exploration of associated intervals through whole-genome comparison of the tolerant accession ‘Kamarat’ and other susceptible accessions, including the PPV-resistant wild-related species P. davidiana, allow the identification of allelic variants in promising candidate genes, including an RTM2-like gene already characterized in A. thaliana.ConclusionsThe present study is the first effort to identify genetic factors involved in Sharka disease in peach germplasm through a GWA approach. We provide evidence of the presence of quantitative resistant loci in a collection of peach accessions, identifying major loci and highly informative SNPs that could be useful for marker assisted selection. These results could serve as reference bases for future research aimed at the comprehension of genetic mechanism regulating the complex peach-PPV interaction.Electronic supplementary materialThe online version of this article (10.1186/s12870-017-1117-0) contains supplementary material, which is available to authorized users.

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“…The apricot cultivar 'Harlayne' was used as a donor of resistance in these populations. It was crossed with two susceptible cultivars, 'Vestar' and 'Strepet' (Polák et al, 2003), and an immune cultivar, 'Orangered' (Fuchs et al, 2001). The crosses were made by hand, without isolation of the fl owers, a er fi rst removing the petals and stamens from the fl ower buds.…”

Section: Plant Materialsmentioning

confidence: 99%

Inheritance of resistance to Plum pox virus in the progeny of the apricot cv. 'Harlayne'

Pilarova¹,

Krška²

2014

Acta Univ. Agric. Silvic. Mendelianae Brun.

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PILAŘOVÁ, P., KRŠKA, B.: Inheritance of resistance to Plum pox virus in the progeny of the apricot cv. 'Harlayne'. Acta univ. agric. et silvic. Mendel. Brun., 2009, LVII, No. 5, pp. 243-250 Natural resistance to Plum pox virus (PPV), the agent of sharka disease, is one of the most important traits of interest to stone fruit breeders, although few sources of resistance have been identifi ed. One of the few apricot cultivars which does show resistance, 'Harlayne', was chosen for a study of the genetics of PPV resistance. It was crossed with three diff erent cultivars, two susceptible ('Vestar' and 'Strepet') and one immune ('Orangered'). Four diff erent lines (since there was one reciprocal combination) were established and the F1 crosses were subsequently inoculated with the PPV-M and PPV-D strains by gra ing infected buds. A woody indicator Prunus persica 'GF 305' was then also top-gra ed onto the plants of three of these F1 populations. The observations of leaf symptoms and accompanying ELISA tests were performed over three, or in one case fi ve, growing seasons and then hybrids were classifi ed accordingly, as either resistant or susceptible. The resistant : susceptible ratios were calculated and compared with expected theoretical ratios using the χ 2 -test. The ratios of resistant to susceptible plants in the progeny derived from the four apricot crosses are compatible with the hypothesis of three dominant genes being responsible for PPV resistance, with 'Harlayne' being heterozygous for all three genes. However, the possibility that resistance is controlled by just two dominant genes can not be ruled out just yet.

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Difference in reactions of apricot and peach cultivars to Plum pox virus: serological and symptomatological evaluation

Cited by 8 publications

References 11 publications

Investigation on the incidence of Plum pox virus in fruit nurseries of the Czech Republic

Investigation on the incidence of Plum pox virus in fruit nurseries of the Czech Republic

Genetic dissection of Sharka disease tolerance in peach (P. persica L. Batsch)

Inheritance of resistance to Plum pox virus in the progeny of the apricot cv. 'Harlayne'

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