Genetic dissection of Sharka disease tolerance in peach (P. persica L. Batsch)

Cirilli, Marco; Rossini, Laura; Geuna, F.; Palmisano, F.; Minafra, A.; Castrignanò, Tiziana; Gattolin, Stefano; Ciacciulli, Angelo; Babini, Anna Rosa; Liverani, A.; Bassi, D.

doi:10.1186/s12870-017-1117-0

Cited by 23 publications

(21 citation statements)

References 73 publications

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“…Tolerance to tomato yellow leaf curl virus is also associated with single dominant genes in wild tomato species and was successfully introgressed into cultivated tomato ( Zamir et al, 1994 ; Vidavsky and Czosnek, 1998 ). In peach, tolerance to plum pox virus (a potyvirus) was mapped to three loci ( Cirilli et al, 2017 ). One of these loci included a candidate gene with similarities to the A. thaliana RTM-2 gene, which is implicated in the restriction of the systemic movement of other potyviruses ( Cirilli et al, 2017 ).…”

Section: Insights In the Complexity Of Tolerant Plant–virus Interactimentioning

confidence: 99%

“…In peach, tolerance to plum pox virus (a potyvirus) was mapped to three loci ( Cirilli et al, 2017 ). One of these loci included a candidate gene with similarities to the A. thaliana RTM-2 gene, which is implicated in the restriction of the systemic movement of other potyviruses ( Cirilli et al, 2017 ). However, functional validation will be required to confirm whether the RTM-2 -like gene is indeed responsible for the tolerance.…”

Section: Insights In the Complexity Of Tolerant Plant–virus Interactimentioning

confidence: 99%

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Exploring the Diversity of Mechanisms Associated With Plant Tolerance to Virus Infection

Paudel

Sanfaçon

2018

Front. Plant Sci.

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Tolerance is defined as an interaction in which viruses accumulate to some degree without causing significant loss of vigor or fitness to their hosts. Tolerance can be described as a stable equilibrium between the virus and its host, an interaction in which each partner not only accommodate trade-offs for survival but also receive some benefits (e.g., protection of the plant against super-infection by virulent viruses; virus invasion of meristem tissues allowing vertical transmission). This equilibrium, which would be associated with little selective pressure for the emergence of severe viral strains, is common in wild ecosystems and has important implications for the management of viral diseases in the field. Plant viruses are obligatory intracellular parasites that divert the host cellular machinery to complete their infection cycle. Highjacking/modification of plant factors can affect plant vigor and fitness. In addition, the toxic effects of viral proteins and the deployment of plant defense responses contribute to the induction of symptoms ranging in severity from tissue discoloration to malformation or tissue necrosis. The impact of viral infection is also influenced by the virulence of the specific virus strain (or strains for mixed infections), the host genotype and environmental conditions. Although plant resistance mechanisms that restrict virus accumulation or movement have received much attention, molecular mechanisms associated with tolerance are less well-understood. We review the experimental evidence that supports the concept that tolerance can be achieved by reaching the proper balance between plant defense responses and virus counter-defenses. We also discuss plant translation repression mechanisms, plant protein degradation or modification pathways and viral self-attenuation strategies that regulate the accumulation or activity of viral proteins to mitigate their impact on the host. Finally, we discuss current progress and future opportunities toward the application of various tolerance mechanisms in the field.

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Section: Insights In the Complexity Of Tolerant Plant–virus Interactimentioning

confidence: 99%

Section: Insights In the Complexity Of Tolerant Plant–virus Interactimentioning

confidence: 99%

Exploring the Diversity of Mechanisms Associated With Plant Tolerance to Virus Infection

Paudel

Sanfaçon

2018

Front. Plant Sci.

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“…An extensive evaluation of a peach gene pool collection (germplasm) showed that PPV resistance is probably quantitatively based and is manifested as a decrease in symptom intensity (Cirilli et al, 2017). For this reason, it is important to disclose the genetic architecture of the response of peach trees and their relative species to PPV infection, which is necessary for pyramiding of resistant genes and for development of more tolerant species and rootstocks.…”

Section: Rootstockmentioning

confidence: 99%

Evaluation of Presence and Concentration of PPV in Rootstocks Derived from Prunus davidiana (Carr.) Franch

Pavelková

Kiss

Nečas

2019

Acta Univ. Agric. Silvic. Mendelianae Brun.

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Evaluation of the presence and concentration of PPV (Plum pox virus) in selected rootstocks was carried out in 2016 - 2017. For the purpose of the experiment we used rootstocks derived from crossbreeding of Prunus davidiana (Carr.) Franch, such as Cadaman and Barrier, and also a P. davidiana seedling. Peach seedling rootstock GF-305 was used as a control. The rootstocks were inoculated artificially with PPV strain M (Marcus). Both the rootstock and the inoculum were tested for presence of the virus by a serological method -semiquantitative DAS-ELISA test and molecular methods -RT-PCR, real-time RT-PCR and RT-LAMP. During the growing season the plants were evaluated for symptom intensity by using a scoring scale. The results show interdependency between symptom intensity and the amount of PPV in leaves, with DAS-ELISA test giving less positive samples than RT-PCR. The RT-LAMP and real-time RT-PCR methods were capable of revealing low concentrations of the virus even in symptom-free plants. The lowest PPV concentrations of all the four rootstocks were detected by real-time RT-PCR in P. davidiana. The highest PPV concentrations were detected in Barrier rootstock. In inocula, the lowest concentration was found in the inocula on Cadaman rootstock, whereas the highest PPV concentration was detected in the inocula inoculated on Barrier rootstock.

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“…The sequencing of 'PI91459' ('NJ Weeping') and 'Bounty' parents was performed at the Genomics Platform of Parco Tecnologico Padano (Lodi, Italy) following the protocol described by Cirilli et al (2017). Functional annotation of the variants was performed using SNPEFFECT 2.0 (Reumers et al, 2006).…”

Section: Whole-genome Sequencingmentioning

confidence: 99%

Deletion of the miR172 target site in a TOE‐type gene is a strong candidate variant for dominant double‐flower trait in Rosaceae

et al. 2018

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Double flowers with supernumerary petals have been selected by humans for their attractive appearance and commercial value in several ornamental plants, including Prunus persica (peach), a recognized model for Rosaceae genetics and genomics. Despite the relevance of this trait, knowledge of the underlying genes is limited. Of two distinct loci controlling the double-flower phenotype in peach, we focused on the dominant Di2 locus. High-resolution linkage mapping in five segregating progenies delimited Di2 to an interval spanning 150 858 bp and 22 genes, including Prupe.6G242400 encoding an euAP2 transcription factor. Analyzing genomic resequencing data from single- and double-flower accessions, we identified a deletion spanning the binding site for miR172 in Prupe.6G242400 as a candidate variant for the double-flower trait, and we showed transcript expression for both wild-type and deleted alleles. Consistent with the proposed role in controlling petal number, Prupe.6G242400 is expressed in buds at critical times for floral development. The indelDi2 molecular marker designed on this sequence variant co-segregated with the phenotype in 621 progenies, accounting for the dominant inheritance of the Di2 locus. Further corroborating the results in peach, we identified a distinct but similar mutation in the ortholog of Prupe.6G242400 in double-flower roses. Phylogenetic analysis showed that these two genes belong to a TARGET OF EAT (TOE)-type clade not represented in Arabidopsis, indicating a divergence of gene functions between AP2-type and TOE-type factors in Arabidopsis and other species. The identification of orthologous candidate genes for the double-flower phenotype in two important Rosaceae species provides valuable information to understand the genetic control of this trait in other major ornamental plants.

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Genetic dissection of Sharka disease tolerance in peach (P. persica L. Batsch)

Cited by 23 publications

References 73 publications

Exploring the Diversity of Mechanisms Associated With Plant Tolerance to Virus Infection

Exploring the Diversity of Mechanisms Associated With Plant Tolerance to Virus Infection

Evaluation of Presence and Concentration of PPV in Rootstocks Derived from Prunus davidiana (Carr.) Franch

Deletion of the miR172 target site in a TOE‐type gene is a strong candidate variant for dominant double‐flower trait in Rosaceae

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