Dietzia natronolimnaios sp. nov., a new member of the genus Dietzia isolated from an East African soda lake

Abstract

Two novel alkaliphilic aerobic organotrophic bacteria have been isolated from a moderately saline and alkaline East African soda lake. The new isolates grow at pH values between 6 and 10, with a pH optimum for growth of 9.0, and at a salt concentration between 0% and 10% (w/v). Phylogenetic analysis based on 16S rDNA sequence shows that these isolates are very closely related (99.6% similarity) and are members of the monospecific genus Dietzia (98.8% and 98.7% similarity). DNA/DNA hybridization revealed a relatedness of 83% between the two isolates, but only 8% between them and the type strain Dietzia maris. The G + C content as measured by thermal denaturation is 66.1 mol%. Phenotypic comparisons between D. maris and one isolate showed that they share very similar morphological and chemotaxonomic properties, but differ significantly in carbon source utilization profiles and halotolerance in alkaline medium. We propose a second species of this genus which we name Dietzia natronolimnaios (type strain 15LN1 = CBS 107.95).

High efficiency degradation of alkanes and crude oil by a salt-tolerant bacterium Dietzia species CN-3

“…Overall, Dietzia sp. CN-3 was able to grow at pH between 5 and 10, with an optimal pH of 7.0, at which Dietzia has the highest enzymatic activity for degradation of the saturated hydrocarbons in crude oil (Duckworth et al, 1998;Wang et al, 2007;Yumoto et al, 2002).…”

Molecular Identification of a Dietzia maris Hip Prosthesis Infection Isolate

“…Dietzia maris has been isolated from soil and from the skin and intestinal tract of a carp (8,9), and Dietzia natronolimnaios was isolated from an east African soda lake (4). Previously classified as Rhodococcus maris (8), D. maris has subsequently been transferred to a new genus, Dietzia, proposed on the basis of the peculiar structure of polar lipids, the presence of short-chain mycolic acids, and 16S ribosomal DNA (rDNA) sequence-based phylogenetic evidence (9).…”

Enhancement of canthaxanthin production from Dietzia natronolimnaea HS-1 in a fed-batch process using trace elements and statistical methods

“…CQ4 (Tao et al, 2007), D. natronolimnaios sp. nov. (Duckworth et al, 1998), Brevibacterium KY-4313 (Nelis and De Leenheer, 1989), Bradyrhizobium strain ORS278 (Hannibal et al, 2000), and Gordonia jacobaea MV-1 (De Miguel et al, 2000); the halophilic archaeon Haloferax alexandrinus TM T (Asker and Ohta, 2002) and various green micro-algae, including Chlorella pyrenoidosa (Czygan, 1964), Chlorococcum sp. strain MA-1 (Zhang and Lee, 2001), and Chlorella zofingiensis (Li et al, 2006), there is clear potential for employing D. natronolimnaea HS-1 to synthesize canthaxanthin.…”

“…CQ4 (Tao et al, 2007), Dietzia natronolimnaios sp. nov. (Duckworth et al, 1998), Gordonia jacobaea (Veiga-Crespo et al, 2005), Bradyrhizobium strain ORS278 (Hannibal et al, 2000), Corynebacterium michiganense (Saperstein and Starr, 1954), Micrococus roseus (Cooney et al, 1996) and Brevibacterium sp. KY-4313 (Nelis and De Leenheer, 1989)), green micro-algae (Chlorococcum sp.…”