2004
DOI: 10.1016/j.eurpolymj.2004.02.015
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Diethylmethyl chitosan as an antimicrobial agent: Synthesis, characterization and antibacterial effects

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Cited by 191 publications
(109 citation statements)
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“…For growth inhibitory concentration (PMIC) the presence of viable microorganisms was tested and the lowest concentration causing bactericidal effect was reported as MBC as suggested by Avadi et al [28]. To test for bactericidal effect, a loopful from each flask was inoculated on nutrient agar and incubated at 35°C for 24 h. The nanoparticle concentration causing bactericidal effect was selected based on absence of colonies on the agar plate.…”
Section: Determination Of Minimum Inhibitory Concentration (Mic) and mentioning
confidence: 99%
“…For growth inhibitory concentration (PMIC) the presence of viable microorganisms was tested and the lowest concentration causing bactericidal effect was reported as MBC as suggested by Avadi et al [28]. To test for bactericidal effect, a loopful from each flask was inoculated on nutrient agar and incubated at 35°C for 24 h. The nanoparticle concentration causing bactericidal effect was selected based on absence of colonies on the agar plate.…”
Section: Determination Of Minimum Inhibitory Concentration (Mic) and mentioning
confidence: 99%
“…The negatively charged surface of the bacterial cell wall interacts effectively to a greater degree with the polycationic nanochitosan (NH 3 + ) and hinders the growth of the microorganism [60]. Chitosan nano-particles provide higher affinity with bacteria cells for a quantum-size effect, due to the larger surface area of the chitosan nanoparticles, which could be tightly adsorbed onto the surface of the bacteria cells to disrupt the membrane, which would lead to the leakage of intracellular components, thus killing the bacteria cells [61,62].…”
Section: Antibacterial Activitymentioning
confidence: 99%
“…Some authors suggested the amino groups of the chitosan when in contact with physiological fluids are protonated and bind to anionic groups of the microorganisms, resulting in the agglutination of the microbial cells, and growth inhibition [49,50]. On the other hand, reference [49] report that when interacting with the bacterial cell, the chitosan, promotes displacement of Ca ++ of the anionic sites of the membrane, damaging them.…”
Section: Properties Antimicrobial and Toxicity Of Fungi Chitosanmentioning
confidence: 99%