Dietary synbiotic application modulates Atlantic salmon (Salmo salar) intestinal microbial communities and intestinal immunity

Abid, Ali; Davies, Simon J.; Waines, Paul; Emery, Matthew; Castex, Mathieu; Gioacchini, Giorgia; Carnevali, Oliana; Bickerdike, Ralph; Romero, Jaime; Merrifield, Daniel L.

doi:10.1016/j.fsi.2013.09.039

Cited by 171 publications

(87 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, there was a greater presence of Tenericutes in samples from aquarium fish whereas Proteobacteria were more abundant in the loch sampled fish. The dominance of these phyla is in agreement with earlier studies on Atlantic salmon and rainbow trout using both DGGE and NGS (Abid et al, 2013, Green et al, 2013, Ingerslev et al, 2014b). This differs from mammals were Firmicutes and Bacteroidetes tend to dominate (Ley et al, 2008).…”

Section: Discussionsupporting

confidence: 91%

“…Metastats analysis found two OTUs associated with the family Mycoplasmataceae significantly more abundant in aquarium samples than loch samples (Supplementary Table S3) and one OTU of the family Mycoplasmataceae unique in fish from the aquarium (Supplementary Table S1). Previous studies found Mycoplasmataceae to be dominant in Atlantic salmon reared in seawater by DGGE and TRF and hence they appear to be a common microbiota member across environments (Abid et al, 2013, Green et al, 2013). Mycoplasmataceae have therefore been suggested as a dominant community member in Atlantic salmon, although the species this family comprises may be host, tissue and habitat specific (Green et al, 2013, Givens et al, 2015).…”

Section: Discussionmentioning

confidence: 91%

“…Genetic background and ontogenic factors are also factors that shape an individuals' gut microbiota (Benson et al, 2010, Ingerslev et al, 2014a). Previous studies showed that fish harbour more specialised gut microbiota than previously assumed (Abid et al, 2013). Furthermore, fish are able to retain specific bacteria, making gut microbial assemblages not a passive collection of seeding communities (Sullam et al, 2012).…”

Section: Introductionmentioning

confidence: 81%

See 2 more Smart Citations

Environmental and physiological factors shape the gut microbiota of Atlantic salmon parr (Salmo salar L.)

2017

View full text Add to dashboard Cite

Gut microbes are key players in host immune system priming, protection and development, as well as providing nutrients to the host that would be otherwise unavailable. Due to this importance, studies investigating the link between host and microbe are being initiated in farmed fish. The establishment, maintenance and subsequent changes of the intestinal microbiota are central to define fish physiology and nutrition in the future. In fish, unlike mammals, acquiring intestinal microbes is believed to occur around the time of first feeding mainly from the water surrounding them and their microbial composition over time is shaped therefore by their habitat.Here we compare the distal intestine microbiota of Atlantic salmon parr reared in a recirculating laboratory aquarium with that of age matched parr maintained in cage culture in an open freshwater loch environment of a commercial fish farm to establish the microbial profiles in the gut at the freshwater stage and investigate if there is a stable subset of bacteria present regardless of habitat type. We used deep sequencing across two variable regions of the 16S rRNA gene, with a mean read depth of 180,144 ± 12,096 raw sequences per sample. All individual fish used in this study had a minimum of 30,000 quality controlled reads, corresponding to an average of 342 ± 19 Operational Taxonomic Units (OTUs) per sample, which predominantly mapped to the phyla Firmicutes, Proteobacteria, and Tenericutes.The results indicate that species richness is comparable between both treatment groups, however, significant differences were found in the compositions of the gut microbiota between the rearing groups. Furthermore, a core microbiota of 19 OTUs was identified, shared by all samples regardless of treatment group, mainly consisting of members of the phyla Proteobacteria, Bacteroidetes and Firmicutes. Core microbiotas of the individual rearing groups were determined (aquarium fish: 19 + 4 (total 23) OTUs, loch fish: 19 + 13 (total 32) OTUs), indicating that microbe acquisition or loss is occurring differently in the two habitats, but also that selective forces are acting within the host, offering niches to specific bacterial taxa.The new information gathered in this study by the Illumina MiSeq approach will be useful to understand and define the gut microbiota of healthy Atlantic salmon in freshwater and expand on previous studies using DGGE, TGGE and T-RFPL. Monitoring deviations from these profiles, especially the core microbes which are present regardless of habitat type, might be used in the future as early indicator for intestinal health issues caused by sub optimal feed or infectious diseases in the farm setting.Statement of relevanceThe Microbiome is central to gut health, local immune function and nutrient up take. We have used deep sequencing approach to show differences in rearing conditions of Atlantic salmon. This work is of interest to aquaculture nutritionists.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 81%

See 1 more Smart Citation

Environmental and physiological factors shape the gut microbiota of Atlantic salmon parr (Salmo salar L.)

2017

View full text Add to dashboard Cite

show abstract

“…Improved intestinal health may increase the ability to absorb digested nutrients, thereby providing the potential to enhance growth performance in fish. These data are in harmony with (Abid et al, 2013) reported that supplementation with a synbiotic that included P. acidilactici and FOS improved the microvilli in rainbow trout.…”

Section: Discussionsupporting

confidence: 89%

Effect of Dietary Vitamin C and and #914;-Glucan to Alleviate the Toxic Effect of Copper Sulphate in Tilapia Fish

El-Keredy¹,

El-Shenway²,

Diab³

et al. 2017

AJVS

View full text Add to dashboard Cite

show abstract

“…This outcome is contrary to that of Jesus et al (), who observed higher width, height, number of villi, and numbers of goblet cells in hybrid surubim ( Pseudoplatystoma reticulatum × P. corruscans ) after receiving the probiotic W. cibaria for 45 days. Other studies have reported higher measurements of gut metrics in fish that received probiotic bacteria, for example, Lee et al () in Anguilla japonica after 60 days of experiment and Abid et al () in Salmon salar after 63 days of feeding with probiotic bacteria. This discrepancy could be attributed to the longer feeding period during which the probiotic had been offered to the fish.…”

Section: Discussionmentioning

confidence: 93%

Autochthonous probiotic bacteria modulate intestinal microbiota of Pirarucu, Arapaima gigas

Pereira

Soares³

et al. 2019

J World Aquaculture Soc

View full text Add to dashboard Cite

This study aimed to investigate the ability of two probiotic strains (Lactococcus lactis subsp. lactis and Enterococcus faecium) to colonize Arapaima gigas intestines and their competence in modulating gut microbiota, morphology, and immunological status. A total of 135 A. gigas (58.86 ± 10.25 g) were divided into three treatments: (a) fish fed a diet containing L. lactis subsp. lactis at 1 × 108 CFU−1, (b) fish fed a diet containing E. faecium at 1 × 108 CFU−1, and (c) fish fed a diet nonsupplemented with probiotic. After 21 days, 12 fish per treatment were sampled. Blood and posterior intestine were sampled for further analysis. High‐throughput sequencing results demonstrated that the relative abundance of Cetobacterium genus was high in all treatments. The number of thrombocytes increased in both groups fed the probiotic, and eosinophils were higher in fish fed E. faecium. Serum antimicrobial activity of fish fed L. lactis subsp. lactis was higher when compared with the other two treatments. In conclusion, both strains could populate the intestinal mucosa of A. gigas and could be used as probiotics in pirarucu production as they can influence hematoimmunological parameters and decrease the abundance of pathogenic bacteria in the gut of pirarucu within 21 days of feeding.

show abstract

Dietary synbiotic application modulates Atlantic salmon (Salmo salar) intestinal microbial communities and intestinal immunity

Cited by 171 publications

References 44 publications

Environmental and physiological factors shape the gut microbiota of Atlantic salmon parr (Salmo salar L.)

Environmental and physiological factors shape the gut microbiota of Atlantic salmon parr (Salmo salar L.)

Effect of Dietary Vitamin C and and #914;-Glucan to Alleviate the Toxic Effect of Copper Sulphate in Tilapia Fish

Autochthonous probiotic bacteria modulate intestinal microbiota of Pirarucu, Arapaima gigas

Contact Info

Product

Resources

About