Dietary supplementation with Weissella cibaria C-10 and Bacillus amyloliquefaciens T-5 enhance immunity against Aeromonas veronii infection in crucian carp (Carassiu auratus)

Zhu, Xin-Kun; Yang, Bintong; Hao, Zhi-Peng; Li, Haizhou; Cong, Wei; Kang, Yuan-Huan

doi:10.1016/j.micpath.2022.105559

Cited by 12 publications

(6 citation statements)

References 58 publications

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“…Microecological preparations can improve the immunity of the host, and other beneficial characteristics, such as a lack of drug resistance, and no toxic side effects ( Mingmongkolchai and Panbangred, 2018 ). At present, probiotic microecological preparations are widely used in aquaculture ( Gopi et al, 2022 ; Zhu et al, 2022 ). However, there is no standard for the selection of probiotics for use in microecological preparations.…”

Section: Discussionmentioning

confidence: 99%

Whole genome analysis of host-associated lactobacillus salivarius and the effects on hepatic antioxidant enzymes and gut microorganisms of Sinocyclocheilus grahami

Xin

Yang

et al. 2022

Front. Microbiol.

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As a fish unique to Yunnan Province in China, Sinocyclocheilus grahami hosts abundant potential probiotic resources in its intestinal tract. However, the genomic characteristics of the probiotic potential bacteria in its intestine and their effects on S. grahami have not yet been established. In this study, we investigated the functional genomics and host response of a strain, Lactobacillus salivarius S01, isolated from the intestine of S. grahami (bred in captivity). The results revealed that the total length of the genome was 1,737,623 bp (GC content, 33.09%), comprised of 1895 genes, including 22 rRNA operons and 78 transfer RNA genes. Three clusters of antibacterial substances related genes were identified using antiSMASH and BAGEL4 database predictions. In addition, manual examination confirmed the presence of functional genes related to stress resistance, adhesion, immunity, and other genes responsible for probiotic potential in the genome of L. salivarius S01. Subsequently, the probiotic effect of L. salivarius S01 was investigated in vivo by feeding S. grahami a diet with bacterial supplementation. The results showed that potential probiotic supplementation increased the activity of antioxidant enzymes (SOD, CAT, and POD) in the hepar and reduced oxidative damage (MDA). Furthermore, the gut microbial community and diversity of S. grahami from different treatment groups were compared using high-throughput sequencing. The diversity index of the gut microbial community in the group supplemented with potential probiotics was higher than that in the control group, indicating that supplementation with potential probiotics increased gut microbial diversity. At the phylum level, the abundance of Proteobacteria decreased with potential probiotic supplementation, while the abundance of Firmicutes, Actinobacteriota, and Bacteroidota increased. At the genus level, there was a decrease in the abundance of the pathogenic bacterium Aeromonas and an increase in the abundance of the potential probiotic bacterium Bifidobacterium. The results of this study suggest that L. salivarius S01 is a promising potential probiotic candidate that provides multiple benefits for the microbiome of S. grahami.

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Section: Discussionmentioning

confidence: 99%

Whole genome analysis of host-associated lactobacillus salivarius and the effects on hepatic antioxidant enzymes and gut microorganisms of Sinocyclocheilus grahami

Xin

Yang

et al. 2022

Front. Microbiol.

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“…Bacillus amyloliquefaciens T-5 could improve the activities of related immune factors, immune enzymes and non-specific immune antibodies in blood and organs (gut, gill, liver, kidney and spleen). Bacillus amyloliquefaciens T-5 could be considered to be developed into microecological preparations for the alternatives of antibiotics in aquaculture (Zhu et al, 2022).…”

Section: Resultsmentioning

confidence: 99%

Pathogenicity analysis and application of probiotic bacteria in Catfish (Clarias sp.) cultivation in vivo

Sukmawati, S.,

Fahrizal, A.,

Yunita, M.

2024

MJM

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Aims:The aim of this study was to analyze the physiological properties, pathogenicity and application of probiotic bacteria to catfish (Clarias sp.) cultivation in vivo. Methodology and results: Probiotic bacteria's physiological characteristics were described using descriptive approaches, whereas experimental methods analyzed pathogenicity tests and the application of the probiotic bacteria in catfish cultivation. The results of bacterial characterization showed that Bacillus safensis strain C251 SA3, Bacillus amyloliquefaciens strain NO10 SA8 and Bacillus australimaris strain IIHR GAPB01 SL1 had bacili cell shape and showed a positive catalase test for all test bacteria except Bacillus safensis strain C251 SA3. The three probiotic bacteria also had negative oxidative-fermentative results, which were able to reduce nitrate and survive in environmental conditions with pH 3-9. Pathogenicity analysis revealed that the three bacteria had the potential to be non-pathogenic or less pathogenic. Bacillus safensis strain C251 SA3 treatment on catfish resulted in the best survival rate of 64% and a 17.5 cm growth in catfish body length by week 13. When Bacillus amyloliquefaciens strain NO10 SA8 was applied to catfish, the survival rate was 32% and the catfish body length increased by 22 cm by week 13. The control treatment revealed that all catfish samples died or had a 0% survival rate, while the application of Bacillus australimaris strain IIHR GAPB01 SL1 revealed a catfish survival rate of 0%. Conclusion, significance and impact of study: Bacillus australimaris strain IIHR GAPB01 SL1 is a potential probiotic bacterium to be applied in catfish food.

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“…Previous studies have reported that the diversity, composition, and relative abundance of intestinal flora are affected by probiotic administration. Feeding probiotics to animals can regulate microbial diversity ( 35 , 36 ). Symbiotic microorganisms play a role in many aspects of animal biology, including digestion and absorption, immune development, behavior, and development.…”

Section: Discussionmentioning

confidence: 99%

Bacillus subtilis M6 improves intestinal barrier, antioxidant capacity and gut microbial composition in AA broiler

Liu

Shen

et al. 2022

Front. Nutr.

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Bacillus subtilis can secret a variety of substances to improve human and animal gut health via inhibiting the proliferation of pathogenic bacteria. In this study, a fast-growing and stress-resistant strain of Bacillus subtilis M6 (B. subtilis M6) were isolated, which showed a strong antibacterial activity to E. coli K88, S. typhimurium ATCC14028, and S. aureus ATCC25923 in vitro. In vivo studies showed that B. subtilis M6 can significantly improve the average daily gain (ADG) using an AA broiler model. Dietary B. subtilis M6 improved the intestinal morphology. The villus height of jejunum and ileum were significantly increased. The concentration of malondialdehyde (MDA) in the ileal mucosa was significantly reduced in B. subtilis M6 treatment group, which suggested the oxidative stress of the ileum was significantly relieved. Though the β diversity of treatments was not significantly, B. subtilis M6 improved the composition of intestinal microbes, especially at the level of caecum genus, the dominant genus was changed from Ruminococcus to Akkermansia, which indicated the change of intestinal carbohydrate nutrition. In conclusion, these data indicate that the B. subtilis M6 shows a probiotic potential to improve intestinal health via altering gut microbiota.

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Dietary supplementation with Weissella cibaria C-10 and Bacillus amyloliquefaciens T-5 enhance immunity against Aeromonas veronii infection in crucian carp (Carassiu auratus)

Cited by 12 publications

References 58 publications

Whole genome analysis of host-associated lactobacillus salivarius and the effects on hepatic antioxidant enzymes and gut microorganisms of Sinocyclocheilus grahami

Whole genome analysis of host-associated lactobacillus salivarius and the effects on hepatic antioxidant enzymes and gut microorganisms of Sinocyclocheilus grahami

Pathogenicity analysis and application of probiotic bacteria in Catfish (Clarias sp.) cultivation in vivo

Bacillus subtilis M6 improves intestinal barrier, antioxidant capacity and gut microbial composition in AA broiler

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