Dietary Supplementation with Spray-Dried Porcine Plasma Attenuates Colon Inflammation in a Genetic Mouse Model of Inflammatory Bowel Disease

Miró, Lluïsa; Amat, Concepció; Rosell-Cardona, Cristina; Campbell, Joy; Polo, Javier; Pérez-Bosque, Anna; Moretó, Miquel

doi:10.3390/ijms21186760

Cited by 13 publications

(16 citation statements)

References 59 publications

(85 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a similar manner to our results, previous studies have shown that SDP supplementation reduces Inos gene expression in a mouse model of acute lung injury [ 21 ], and other natural products such as curcumin can inhibit this gene expression in microglia [ 62 ]. This relationship between neuroinflammation and activated microglia might explain the reduction in microglia markers by SDP supplementation, as SDP supplementation reduces both neuronal and systemic inflammation [ 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…SDP promotes growth performance, feed efficiency, and animal survival [ 15 , 16 ]. Furthermore, SDP exerts anti-inflammatory effects in different models of inflammation, such as in the intestinal [ 17 , 18 , 19 , 20 ], pulmonary [ 21 ], and genitourinary mucosae in a mouse model, inducing low pregnancy rates by stress [ 22 ]. It also stimulates the growth of probiotic species in the gut microbiota and the expression of mucosal regulators of anti-inflammatory pathways [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dietary Spray-Dried Porcine Plasma Reduces Neuropathological Alzheimer’s Disease Hallmarks in SAMP8 Mice

et al. 2021

Self Cite

View full text Add to dashboard Cite

Alzheimer’s disease (AD) is characterized by the aberrant processing of amyloid precursor protein (APP) and the accumulation of hyperphosphorylated tau, both of which are accompanied by neuroinflammation. Dietary supplementation with spray-dried porcine plasma (SDP) has anti-inflammatory effects in inflammation models. We investigated whether dietary supplementation with SDP prevents the neuropathological features of AD. The experiments were performed in 2- and 6-month-old SAMP8 mice fed a control diet, or a diet supplemented with 8% SDP, for 4 months. AD brain molecular markers were determined by Western blot and real-time PCR. Senescent mice showed reduced levels of p-GSK3β (Ser9) and an increase in p-CDK5, p-tau (Ser396), sAPPβ, and the concentration of Aβ40, (all p < 0.05). SDP prevented these effects of aging and reduced Bace1 levels (all p < 0.05). Senescence increased the expression of Mme1 and Ide1 and pro-inflammatory cytokines (Il-17 and Il-18; all p < 0.05); these changes were prevented by SDP supplementation. Moreover, SDP increased Tgf-β expression (p < 0.05). Furthermore, in aged mice, the gene expression levels of the microglial activation markers Trem2, Ym1, and Arg1 were increased, and SDP prevented these increases (all p < 0.05). Thus, dietary SDP might delay AD onset by reducing its hallmarks in senescent mice.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dietary Spray-Dried Porcine Plasma Reduces Neuropathological Alzheimer’s Disease Hallmarks in SAMP8 Mice

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, it is widely accepted that health-promoting effect of SDP is mainly attributed to the immunomodulatory property of SDP on the intestinal barrier [3,6,31]. The anti-inflammatory activities of SDP including suppressing pro-inflammatory cytokine production (e.g., TNF-α and IFN-γ), stimulating anti-inflammatory cytokine production (e.g., IL-10 and TGF-β), inhibiting inflammatory cell infiltration in the gut mucosa and lamina propria, and improving intestinal barrier function are well-recognized especially in rodent models [32][33][34][35][36][37], and in weaned pigs to a lesser extent [8,38]. Continuous exposure of the intestinal mucosa to bacterial pathogens or toxins can cause chronic overstimulation of the mucosal immune system, thereby impairing intestinal barrier function and resulting in poor nutrient absorption.…”

Section: Discussionmentioning

confidence: 99%

Effects of spray-dried animal plasma on growth performance, survival, feed utilization, immune responses, and resistance to Vibrio parahaemolyticus infection of Pacific white shrimp (Litopenaeus vannamei)

et al. 2021

View full text Add to dashboard Cite

Spray-dried animal plasma (SDP) in feed for several animal species provides health benefits, but research about use of SDP in shrimp feed is very limited. The objectives of the present study were to investigate the effects of dietary SDP on growth performance, feed utilization, immune responses, and prevention of Vibrio parahaemolyticus infection in Pacific white shrimp (Litopenaeus vannamei). In Experiment 1, the post-larvae were divided into five groups (four tank/group and 80 shrimp/tank) and fed four times daily diets with porcine SDP at 0, 1.5, 3, 4.5, and 6% of the diet for 45 days. In Experiment 2, the surviving shrimp from Experiment 1 were redistributed into six groups: four SDP groups as in Experiment 1 plus the positive and negative controls (four tank/group and 30 shrimp/tank). They were then challenged with V. parahaemolyticus by immersion at 105 colony-forming units (CFU)/mL and were fed with the same diets for another 4 days. In Experiment 1, shrimp fed 4.5% or 6% SDP diets had significantly higher body weight, survival rate, and improved feed conversion ratio. The immune parameters (total hemocyte count and phagocytic, phenoloxidase, and superoxide dismutase activities) of the shrimp fed 3–6% SDP diets also showed significant enhancement compared to the control. In Experiment 2, the survival rates of the 3–6% SDP groups were significantly higher than the positive control at day 4 after the immersion challenge. Likewise, the histopathological study revealed milder signs of bacterial infection in the hepatopancreas of the 3–6% SDP groups compared to the challenged positive control and 1.5% SDP groups. In conclusion, shrimp fed diets with SDP, especially at 4.5–6% of the diet, showed significant improvement in overall health conditions and better resistance to V. parahaemolyticus infection.

show abstract

“…Namely, in young and senescent animals challenged by Staphylococcus aureus enterotoxin B, dietary supplementation with functional proteins from SDP of porcine origin has been shown to modulate the intestinal barrier and defense mechanisms, thereby reducing the degree of gut-associated lymphoid tissue activation [ 2 , 8 , 9 ]. A dietary supplement containing over 90% bovine serum protein (>50% immunoglobulins) reduces inflammatory markers and tissue damage in mice models of colitis [ 10 , 11 , 12 ] and mucositis [ 13 ]. Simultaneous protection against lung and colon inflammation has been documented for porcine SDP [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes

Hernández‐Chirlaque

Aranda

Ocón

et al. 2021

Animals

Self Cite

View full text Add to dashboard Cite

Serum protein concentrates have been shown to exert in vivo anti-inflammatory effects. Specific effects on different cell types and their mechanism of action remain unraveled. We aimed to characterize the immunomodulatory effect of two porcine plasma protein concentrates, spray dried serum (SDS) and an immunoglobulin concentrate (IC), currently used as animal nutritional supplements with established in vivo immunomodulatory properties. Cytokine production by the intestinal epithelial cell line IEC18 and by primary cultures of rat splenocytes was studied. The molecular pathways involved were explored with specific inhibitors and gene knockdown. Our results indicate that both products induced GROα and MCP-1 production in IEC18 cells by a MyD88/NF-κB-dependent mechanism. Inhibition of TNF production was observed in rat primary splenocyte cultures. The immunoglobulin concentrate induced IL-10 expression in primary splenocytes and lymphocytes. The effect on TNF was independent of IL-10 production or the stimulation of NF-kB, MAPKs, AKT, or RAGE. In conclusion, SDS and IC directly regulate intestinal and systemic immune response in murine intestinal epithelial cells and in T lymphocytes and monocytes.

show abstract

Dietary Supplementation with Spray-Dried Porcine Plasma Attenuates Colon Inflammation in a Genetic Mouse Model of Inflammatory Bowel Disease

Cited by 13 publications

References 59 publications

Dietary Spray-Dried Porcine Plasma Reduces Neuropathological Alzheimer’s Disease Hallmarks in SAMP8 Mice

Dietary Spray-Dried Porcine Plasma Reduces Neuropathological Alzheimer’s Disease Hallmarks in SAMP8 Mice

Effects of spray-dried animal plasma on growth performance, survival, feed utilization, immune responses, and resistance to Vibrio parahaemolyticus infection of Pacific white shrimp (Litopenaeus vannamei)

Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes

Contact Info

Product

Resources

About