Dietary Supplementation With High Fiber Alleviates Oxidative Stress and Inflammatory Responses Caused by Severe Sepsis in Mice Without Altering Microbiome Diversity

Abstract

In this study, we demonstrated the effects of a high-fiber diet on intestinal lesions, oxidative stress and systemic inflammation in a murine model of endotoxemia. C57BL/6 mice were randomly assigned to four groups: the control group (CONTROL), which received a commercial normal-fiber rodent diet comprising normal fiber; a CLP group, which received a commercial normal-fiber rodent diet and underwent caecal ligation puncture (CLP); a high-fiber group (HFG), which received a commercial high-fiber rodent diet; and a high fiber + CLP group (HFCLP) which received a commercial high-fiber rodent diet and underwent CLP (30%). The sepsis model was created via CLP after 2 weeks of dietary intervention. Notably, dietary high-fiber supplementation in HFCLP group improved survival rates and reduced bacterial loads, compared with CLP alone. In the HFCLP group, dietary fiber supplementation decreased the serum concentrations of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6) and high-mobility group protein 1 (HMG-1) but raised the concentration of interleukin 10 (IL-10), compared with the levels in CLP mice. Meanwhile, high-fiber supplementation increased the relative proportions of Akkermansia and Lachnospiraceae. These data show that dietary high-fiber supplementation may be therapeutic for sepsis-induced lesions.

[Retracted] Protective Effects of Hydrogen on Myocardial Mitochondrial Functions in Septic Mice

“…e CLP procedure is a well-established, clinically germane method for generating models used in sepsis research [8,9]. Mice with severe CLP-induced sepsis exhibit increased damage, compared with nonseptic mice.…”

“…For example, treatment with zinc protoporphyrin IX (ZnPPIX) prior to cecal ligation and puncture (CLP) was associated with increased proinflammatory cytokine levels 6 h after the procedure [7]. In addition, nuclear factor erythroid 2-related factor 2 (Nrf2) is increasingly recognized as an important regulator of basal and induced expression of a range of antioxidant response element-dependent genes [8,9]. us, Nrf2 regulates both physiological and pathophysiological responses to the presence of oxidants [10].…”

Chlorogenic Acid Ameliorates Colitis and Alters Colonic Microbiota in a Mouse Model of Dextran Sulfate Sodium-Induced Colitis

“…Studies have shown that the oral administration of DSS can reduce the abundance of Akkermansia muciniphila and Bacteroides acidifaciens in feces (Kang et al, 2013; Yin et al, 2018). Recent studies of an IL10 -/- mouse model of IBD have identified NLRP6 as an important inhibitor of spontaneous colitis, whereas a lack of NLRP6 leads to the enrichment of Akkermansia muciniphila (Seregin et al, 2017; Zhang et al, 2018). Further studies of mouse models of colitis found that Lactobacillus paracasei could reduce intestinal inflammation and the expression of pro-inflammatory factors in the mucosa (Oliveira et al, 2011; Wu and Tian, 2017; Azad et al, 2018).…”

Biological Effects and Applications of Chitosan and Chito-Oligosaccharides

“…Chronic inflammation of the gut is involved in various forms of inflammatory bowel disease (IBD), such as Crohn’s disease and ulcerative colitis (Herfarth, 2013; Hill, 2014; Guan and Lan, 2018; Hong and Piao, 2018; Zhang Y. et al, 2018). The occurrence of IBD has steadily increased in certain parts of the world in the last 40 years, perhaps as a result of changing dietary practices, including the preference for low-fiber diets (Rose et al, 2007; Umakanthan et al, 2016; Weichselbaum and Klein, 2018; Zhang X. et al, 2018).…”