Dietary Supplementation with Butyrate and Polyhydroxybutyrate on the Performance of Pacific White Shrimp in Biofloc Systems

Abstract

This study aimed to evaluate the effects of dietary supplementation with sodium butyrate or polyhydroxybutyrate (PHB) on growth performance, as well as changes in intestinal microbiota and hemato‐immunological parameters, of Litopenaeus vannamei reared under a superintensive biofloc system. Twelve 800‐L tanks were each stocked with 250 shrimp/m3 (3.96 ± 0.04 g mean initial weight) and reared over a 6‐wk period. The basal diet and two test diets supplemented with 2% of each feed additive. At the conclusion of the growth trial shrimp fed with the butyrate‐supplemented diet, as compared with the control shrimp, showed higher survival and productivity and lower total bacterial and Thiosulfate‐citrate‐bile salts‐sucrose Agar (TCBS) counts in the intestine. However, no differences were observed in other performance parameters analyzed. Shrimp fed with both supplementation regimens also showed an increase in total and granular hemocytes, as well as an increase in serum agglutination titer. Shrimp offered diets supplemented with sodium butyrate had higher counts of hyaline cells. Thus, for L. vannamei reared in a superintensive biofloc system, it can be concluded that dietary supplementation of sodium butyrate, more so than PHB, acted as an immune system modulator by reducing the concentration of pathogenic bacteria in shrimp gut, thereby increasing survival and productivity.

Effects of dietary supplementation of monobutyrin and tributyrin on growth, feed efficiency, innate immunity, digestibility and disease resistance of Pacific white shrimp ( Litopenaeus vannamei ) against Vibrio harveyi

“…Silva et al (2014) found that a sodium butyrate addition (5–20 g/kg) in diet reduced intestinal Vibrio counts in L. vannamei than sodium propionate supplementation (5–20 g/kg) in diet. The intestinal bacterial count was decreased in shrimp fed diet containing 20 g/kg sodium butyrate showing lower counts of Vibrio (Silva, Jatobá, et al, 2016). Therefore, it is assumed that the efficiency of butyric acid glycerol esters in the present study was because of high levels of butyric acid release in the intestinal tract of shrimp through optimized free organic acids release by endogenous lipases and esterases.…”

Benzoic Acid Inclusion Effects on Health Status and Growth Performance of Juvenile Pacific White Shrimp Penaeus vannamei

“…Benzoic acid inclusion in the commercial diet is a simple process in which the original components are not altered. The shrimp that were used throughout the experiment were found to be active during feeding time, consuming the pellets as reported in feeding bioassays with several salts of OAs for Pacific white shrimp (da Silva et al 2013Silva et al , 2016aSilva et al , 2016b.…”

The effects of dietary poly‐β‐hydroxybutyrate on growth parameters, intestinal microflora, and histopathology of rainbow trout, Oncorhynchus mykiss, fingerlings

“…It was also demonstrated in previous studies that the PHB had inert properties on the intestinal microflora of experimental animals. For instance, Da Silva et al (2016) investigated the effects of dietary butyrate and PHB on the performance of Pacific white shrimp and found that the shrimps fed with a 2% PHB diet did not alter significantly in intestinal bacterial counts. In another research, the intestinal microbiota of Siberian sturgeon larvae fed with PHB enriched Artemia nauplii did not differ concerning the control group (Najdegerami et al, 2015).…”