Dietary supplementation of fish protein hydrolysate improves growth, feed efficiency and immune response in freshwater carnivore fish, Channa striata fingerlings

Abstract

The effect of dietary fish protein hydrolysate (FPH) at inclusion levels of 0% (control), 5% (FPH5), 10% (FPH10) and 15% (FPH15) (w/w) on growth, digestive enzyme activity, immune response and antioxidant status of striped murrel, Channa striata (4.18 ± 0.04 g), was evaluated. The dietary FPH inclusion had significantly increased weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER) and decreased feed conversion ratio (FCR) compared with the control (p < 0.05). The specific activities of digestive enzymes (amylase and total protease) and transaminases (AST and ALT) were similar in all the treatment groups (p > 0.05). The inclusion of dietary FPH had no influence on SOD and NBT (p > 0.05). The antioxidant enzyme catalase was lower in the FPH5 group than in other treatment groups. Fish fed a diet supplemented with FPH had higher haemoglobin, WBC and albumin levels than the control group, indicating better health. The fish fed FPH10 displayed significantly improved serum lysozyme and myeloperoxidase compared with control, FPH5 and FPH15. Remarkably, qRT‐PCR revealed significant upregulation of IGF‐I in FPH10 and FPH15; complement component C3 in FPH10; and glutathione S‐transferase (GST) in the fish fed FPH15 when compared to the control group. Based on the results of growth performance and immune response, it can be concluded that FPH at 10% may be recommended in the diet of striped murrel fingerlings.

Using Azadirachta indica protein hydrolysate as a plant protein in Nile tilapia (Oreochromis niloticus) diet: Effects on the growth, economic efficiency, antioxidant‐immune response and resistance to Streptococcus agalactiae

“…The increased FCs by AIPH inclusion were due to the increased TFI translated into superior growth capabilities. Our results concurred with earlier findings of Siddaiah et al (2022).…”

“…The latter, activated during hydrolysis with their hydrophobic features, can enhance their interaction with lipids and support their greater antioxidant properties (Wen et al, 2020). These findings were supported by earlier researches (Fan et al, 2022; Shaghayegh et al, 2019; Siddaiah et al, 2022). Additionally, it was reported by Arise et al (2019) that AIPH generates peptide fractions with antioxidant characteristics.…”

“…The augmented parameters supposedly enhanced hydrolyzed protein absorption and improved fish health overall, including immune status (Khosravi et al, 2015). In this regard, Siddaiah et al (2022) observed similar findings regarding the blood indices of striped murrel ( Channa striata ).…”

“…These outcomes come from the AAs and bioactive peptides of AIPH that have immunomodulatory and antioxidant characteristics (Kiewiet et al, 2018;Ndiaye et al, 2012). Similarly, the immune response of P. major and C. striata augmented in response to different dietary protein hydrolysates, as reported by Khosravi et al (2015) and Siddaiah et al (2022).…”

Effects of dietary hydrolysate supplementation on growth, body composition, hematological responses, and liver histology of juvenile giant trevally (Caranx ignobilis Forsskal, 1775)

“…On the other hand, whole‐body lipid of Japanese flounder increased with increasing FPH inclusions from 23 to 45 g/kg and then declined with a further increase in FPH from 67 to 90 g/kg (Zheng et al, 2014). Siddaiah et al (2022) also reported increased lipid in striped murrel Chana striata fed diet containing 100 g/kg FPH, while higher dietary FPH supplementation of 150 g/kg resulted in a reduced lipid level. A similar trend was reported in turbot (Wei et al, 2016; Xu et al, 2016) and pike silverside Chirostoma estor (Ospina‐Salazar et al, 2016) as whole‐body lipid levels reduced at certain dietary FPH levels (Espe et al, 2012; Xu et al, 2016; Zheng et al, 2014).…”