Dietary supplementation of Bacillus subtilis PB6 improves sow reproductive performance and reduces piglet birth intervals

Abstract

We investigated the effects of dietary supplementation with Bacillus subtilis PB6 ( B. subtilis PB6) during late gestation and lactation on sow reproductive performance, antioxidant indices, and gut microbiota. A total of 32 healthy Landrace × Yorkshire sows on d 90 of gestation were randomly assigned to 2 groups, with 16 replicates per group, receiving basal diet (CON) or the basal diet + 0.2% B. subtilis PB6, containing 4.0 × 10 8 CFU/kg of feed (BS). The litter sizes (total born) and numbers of piglets born alive were larger in the BS group ( P < 0.01), whereas the weights of piglets born alive and the piglet birth intervals were lower in the BS group ( P < 0.05). Although the litter weights and piglet bodyweights (after cross-fostering) were lower after BS treatment ( P < 0.05), the litter sizes, litter weights, lactation survival rate, and litter weight gains at weaning were higher in BS group ( P < 0.05). The concentrations of malondialdehyde (MDA) in the sow sera at parturition were lower in the BS group ( P < 0.01). The serum total antioxidant capacity (T-AOC) at parturition and the serum catalase (CAT) concentrations on d 21 of lactation were higher in the BS group ( P < 0.05). Dietary supplementation with B. subtilis PB6 ( P < 0.05) reduced the serum endotoxin concentrations in the sows and the serum cortisol concentrations of the piglets at d 14 of lactation. The α-diversity indices of microbial were higher in the CON group ( P < 0.05). At the phylum level, B. subtilis PB6 supplementation increased the relative abundances of Gemmatimonadete and Acidobacteria (both P < 0.01) and reduced those of Proteobacteria, and Actinobacteria (both P < 0.05). At the genus level, B. subtilis PB6 supplementation increased the relative abundance of Ruminococcaceae_UCG-013 cc ( P < 0.05) and reduced that of Streptococcus ( P < 0.05). This study demonstrated that adding 4.0 × 10 8 CFU/kg B. subtilis PB6 to sows’ feed during late gestation and lactation could shorten piglet birth intervals, enhance the growth performance of suckling piglets, and improve the gut health of sows during late gestation.

Galacto-oligosaccharides improve barrier function and relieve colonic inflammation via modulating mucosa-associated microbiota composition in lipopolysaccharides-challenged piglets

“…The increased relative abundance of Firmicutes is beneficial to intestinal health, as it contains a large number of SCFA-producing bacteria, such as butyrate-producer Eubacterium rectale and Eubacterium Hallii , which help to maintain intestinal health [ 30 ]. Actinobacteria is reported to be part of the symbiosis intestinal microbiota, but a high proportion of Actinobacteria is associated with intestinal inflammatory diseases and colon cancer [ 31 ]. Therefore, our results suggested that GOS could increase the relative abundance of Firmicutes and decrease the relative abundance of Actinobacteria in the colonic mucosa of LPS-challenged piglets.…”

Galacto-Oligosaccharides Improve Barrier Function and Relieve Colonic Inflammation via Modulating Mucosa-Associated Microbiota Composition in Lipopolysaccharides-Challenged Piglets

“…Increased relative abundance of Firmicutes is bene cial to intestinal health, as it contains a large number of SCFAproducing bacteria, such as butyrate-producer Eubacterium rectale and Eubacterium Hallii, which help to maintain intestinal health 21 . Actinobacteria is reported to be part of the symbiosis intestinal microbiota, but a high proportion of Actinobacteria is associated with intestinal in ammatory diseases and colon cancer 22 . Therefore, our results suggested that GOS could increased the relative abundance of Firmicutes and decreased the relative abundance of Actinobacteria in the colonic mucosa of LPSchallenged piglets.…”

Avaliação do uso de blend de levedura em rações para suínos de fase de creche, crescimento e terminação

“…Existem diversos tipos de microrganismos utilizados como probióticos, com destaque são os dos gêneros Lactobacillus, Bifidobacterium, Enterococcus, Bacillus e leveduras (Zhang et al, 2020). Existem peculiaridades dos microrganismos para que esses possam vir a ser utilizados como probióticos, são: fazer parte da flora já existente no hospedeiro, ter alta capacidade de sobrevivência e colonização, ter boa capacidade de ligação ao epitélio intestinal, sobreviver à ação de enzimas digestivas do hospedeiro, não ser tóxicos ou patogênicos, ser cultivável em escala industrial, ser comerciável e estimular a capacidade imune do hospedeiro (Souza et al, 2020).…”