Dietary rescue of altered metabolism gene reveals unexpected Drosophila mating cues

Bousquet, François; Chauvel, Isabelle; Flaven‐Pouchon, Justin; Farine, Jean‐Pierre; Ferveur, Jean‐François

doi:10.1194/jlr.m064683

Cited by 15 publications

(10 citation statements)

References 29 publications

(28 reference statements)

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“…Given that the microbiota and nutrient quality of the juvenile diet can affect reproduction 18 , 19 , we compared the mating ability, fertility, fecundity, and production of cuticular pheromones in male and female flies raised on the different juvenile diet treatments.…”

Section: Resultsmentioning

confidence: 99%

Yeast quality in juvenile diet affects Drosophila melanogaster adult life traits

Grangeteau

Yahou

Everaerts

et al. 2018

Sci Rep

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Diet quality is critical for animal development and survival. Fungi can provide nutrients that are essential to organisms that are unable to synthetize them, such as ergosterol in Drosophila melanogaster. Drosophila studies examining the influence of yeast quality in the diet have generally either provided the diet over the whole life span (larva to adult) or during the adult stage and have rarely focussed on the juvenile diet. Here, we tested the effect of yeast quality in the larval diet on pre-adult development and adult weight, survival, reproduction and food preference. The yeast Saccharomyces cerevisiae was added in three forms in three treatments—live, heated or dried—to food used as the juvenile diet or was not added (empty treatment). Adults resulting from the larvae raised on these four juvenile diets were all maintained on a similar standard laboratory food diet. Our data indicate that yeast quality in the juvenile diet affects larva-to-pupa—but not pupa-to-adult—development. Importantly, adult survival, food preference, mating behaviour and cuticular pheromones strongly varied with the juvenile diet. Therefore, the variation of yeast quality in the pre-adult Drosophila diet affects key adult life traits involved in food search, reproduction and survival.

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Section: Resultsmentioning

confidence: 99%

Yeast quality in juvenile diet affects Drosophila melanogaster adult life traits

Grangeteau

Yahou

Everaerts

et al. 2018

Sci Rep

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“…To yield adults, 6908/RNAi larvae were raised on plain food enriched with oleic acid (“L”)39. Some 6908/RNAi individuals also received this diet during adult life (“L + A”).…”

Section: Resultsmentioning

confidence: 99%

“…The UAS- desat1 -RNAi was also targeted by the complete desat1 regulatory region (6908-Gal43238). Since 6908/RNAi individuals die during larval development, we rescued adulthood by feeding larvae with oleic acid (C18:1; 500 mg/ml) added in the lab food39. After emergence, rescued 6908/RNAi adults were either shifted on standard food or kept on C18:1-rich food.…”

Section: Methodsmentioning

confidence: 99%

Free flight odor tracking in Drosophila: Effect of wing chemosensors, sex and pheromonal gene regulation

Houot

Gigot

Robichon

et al. 2017

Sci Rep

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The evolution of powered flight in insects had major consequences for global biodiversity and involved the acquisition of adaptive processes allowing individuals to disperse to new ecological niches. Flies use both vision and olfactory input from their antennae to guide their flight; chemosensors on fly wings have been described, but their function remains mysterious. We studied Drosophila flight in a wind tunnel. By genetically manipulating wing chemosensors, we show that these structures play an essential role in flight performance with a sex-specific effect. Pheromonal systems are also involved in Drosophila flight guidance: transgenic expression of the pheromone production and detection gene, desat1, produced low, rapid flight that was absent in control flies. Our study suggests that the sex-specific modulation of free-flight odor tracking depends on gene expression in various fly tissues including wings and pheromonal-related tissues.

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“…Metabolic deficit is one of the important reasons for the lethal phenotype of DESAT1 -deficient or -silenced D. melanogaster larvae. However, artificial diet supplemented with unsaturated fatty acids can rescue the survival of larvae [49]. Knockdown of Nlug-desatA1-b , Nlug-desatA1-c , and Nlug-Ifc not only significantly down-regulated unsaturated and saturated fatty acid composition but also significantly reduced the survival rate of BPH (Figure 7 and Figure 8).…”

Section: Discussionmentioning

confidence: 99%

The Desaturase Gene Family is Crucially Required for Fatty Acid Metabolism and Survival of the Brown Planthopper, Nilaparvata lugens

Zeng

Noman

et al. 2019

IJMS

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Desaturases are essentially required for unsaturated fatty acid (UFA) biosynthesis. We identified 10 genes encoding putative desaturases in the transcriptome database of the brown planthopper (BPH), Nilaparvata lugens. These include eight First Desaturase family genes, one cytochrome b5 fused desaturase gene (Nlug-Cytb5r) and one Sphingolipid Desaturase gene (Nlug-ifc). Transcript level profiling revealed significant variation in the expression patterns of these genes across tissues and developmental stages, which occur in a gene-specific manner. Interestingly, their expression was also modulated by the insect food source: the mRNA levels of Nlug-desatC and Nlug-Cytb5r were down-regulated, but the expression level of Nlug-desatA1-b and Nlug-desatA1-c were elevated in the BPH fed on the resistant rice variety Babawee as compared to the non-resistant variety Taichun Native 1 (TN1). Silencing Nlug-desatA1-b, Nlug-desatA1-c, or Nlug-Ifc reduced fatty acid composition and abundance in female BPH 1-d-old-adults compared to controls. Whereas, single knockdown of all ten desaturase genes significantly increased mortality of BPH nymphs compared with controls. Of the ten desaturase genes, knockdown of Nlug-desatA1-b and Nlug-desatA2 caused the highest mortality in BPH (91% and 97%, respectively). Our findings offer a base for expression and functional characterization of newly identified desaturase genes in BPH, and may contribute to RNA interference-based pest management strategies.

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Dietary rescue of altered metabolism gene reveals unexpected Drosophila mating cues

Cited by 15 publications

References 29 publications

Yeast quality in juvenile diet affects Drosophila melanogaster adult life traits

Yeast quality in juvenile diet affects Drosophila melanogaster adult life traits

Free flight odor tracking in Drosophila: Effect of wing chemosensors, sex and pheromonal gene regulation

The Desaturase Gene Family is Crucially Required for Fatty Acid Metabolism and Survival of the Brown Planthopper, Nilaparvata lugens

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