Dietary Phospholipid-Dependent Reductions in Gene Expression and Activity of Liver Enzymes in Fatty Acid Synthesis in Fasted-Refed Rats.

Rouyer, Isabelle A.; Takahashi, Yōko; Ishii, Takashi

doi:10.3177/jnsv.45.287

Cited by 13 publications

(10 citation statements)

References 29 publications

(34 reference statements)

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“…Although the doses of fructose used in this study are widely used in studies of fructose, we have to note that they are above the physiological range. Consistent with previous reports (22,23), PL ingestion modulated the expression of lipogenic genes in the liver; gene expression and activities of the enzymes involved in fatty acid synthesis, FAS and ACC, were lower in rats fed the F-PL diet than in rats fed the F diet. PL ingestion also decreased SCD-1 and ELOVL6, which are involved in the modification of fatty acids.…”

Section: Discussionsupporting

confidence: 92%

“…PL from soybeans have hypolipidemic effects in humans and rodents (20,21). PL ingestion also suppresses mild hepatic TG accumulation through the inhibition of lipogenesis in the fastedrefed model (22,23). These findings suggest that the ingestion of PL is effective against fructose-induced hepatic steatosis and subsequent insulin resistance, but the effects of PL are not well understood.…”

Section: Introductionmentioning

confidence: 76%

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Dietary Phospholipids Ameliorate Fructose-Induced Hepatic Lipid and Metabolic Abnormalities in Rats

Mori

Kondo

Hase

et al. 2011

The Journal of Nutrition

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Overconsumption of fructose results in hepatic dyslipidemia, which has a documented correlation with metabolic syndrome. We examined whether the ingestion of phospholipids (PL) from soybeans prevents fructose-induced metabolic abnormalities. Rats were fed either a fructose-free diet (C), a 60% fructose diet (F), or a 60% fructose plus 3% PL diet (F-PL) for 10 wk. At wk 8, plasma glucose concentrations after glucose loading were significantly higher in rats fed the F diet than in rats fed the C and F-PL diets, which did not differ from one another. The concentrations of hepatic TG, diglycerides, ceramides, and oleates in rats fed the F diet for 10 wk was significantly higher than those in rats fed the C diet. The increases were prevented by concurrent PL ingestion; concentrations did not differ between the F-PL and C groups. Dietary fructose increased the mRNA expression of SREBP1, ChREBP, and genes related to lipogenesis. PL completely inhibited these increases. Furthermore, reflecting the difference at the mRNA level, lipogenic enzyme activities were greater in rats fed the F diet than in rats fed the C diet, and PL ingestion suppressed the increased activities by fructose feeding. Treatment of cultured Hep-G2 cells with fructose for 24 h increased the levels of SREBP1 and ChREBP nuclear proteins, which were suppressed by culture with purified PL components, especially phosphatidylethanolamine and phosphatidylinositol. These findings indicate that PL prevents fructose-induced metabolic abnormalities in association with alterations of the hepatic lipid profile by inhibiting de novo lipogenesis.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 76%

Dietary Phospholipids Ameliorate Fructose-Induced Hepatic Lipid and Metabolic Abnormalities in Rats

Mori

Kondo

Hase

et al. 2011

The Journal of Nutrition

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“…Rat liver was homogenized in 0.2 M HEPES (pH 7.4) containing 0.1 mM EDTA and 10 mM mercaptoethanol, centrifuged at 20,000× g for 30 min at 49 C. The activities of acetyl-CoA carboxylase, FAS, malic enzyme, and glucose-6-phosphate dehydrogenase (G6PDH) were measured as detailed previously. [7][8][9][10] The activity of ATP-citrate lyase was measured according to the procedure described by Takeda et al…”

mentioning

confidence: 99%

Conjugated Linoleic Acid (CLA) Inhibits Fatty Acid Synthetase Activityin Vitro

Oku

Wongtangtintharn

Iwasaki

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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“…(29,30) It has been reported that the expressions of the genes of malic enzyme 1 (31) as well as fatty acid desaturases (29,30) are dually regulated by SREBP-1 and PPARα. Our previous studies showed that both sesamin (3,26) and soybean phospholipid (7,8,10) decreased the activity and mRNA levels of many enzymes involved in hepatic lipogenesis. These previous findings were also confirmed in the present study (Fig.…”

Section: Discussionmentioning

confidence: 97%

“…(6–10) In relation to this, we previously demonstrated that soybean phospholipid profoundly and dose-dependently decreased the activity and mRNA levels of lipogenic enzymes in rat liver. (7,8,10) …”

Section: Introductionmentioning

confidence: 85%

Combined effect of sesamin and soybean phospholipid on hepatic fatty acid metabolism in rats

Ishii

2014

J. Clin. Biochem. Nutr.

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We studied the combined effect of sesamin (1:1 mixture of sesamin and episesamine) and soybean phospholipid on lipid metabolism in rats. Male rats were fed diets supplemented with 0 or 2 g/kg sesamin, and containing 0 or 50 g/kg soybean phospholipid, for 19 days. Sesamin and soybean phospholipid decreased serum triacylglycerol concentrations and the combination of these compounds further decreased the parameter in an additive fashion. Soybean phospholipid but not sesamin reduced the hepatic concentration of triacylglycerol. The combination failed to cause a strong decrease in hepatic triacylglycerol concentration, presumably due to the up-regulation of Cd36 by sesamin. Combination of sesamin and soybean phospholipid decreased the activity and mRNA levels of hepatic lipogenic enzymes in an additive fashion. Sesamin strongly increased the parameters of hepatic fatty acid oxidation enzymes. Soybean phospholipid increased hepatic activity of 3-hydroxyacyl-CoA dehydrogenase although it failed to affect the activity of other enzymes involved in fatty acid oxidation. Sesamin strongly increased hepatic concentration of carnitine. Sesamin and soybean phospholipid combination further increased this parameter, accompanying a parallel increase in mRNA expression of carnitine transporter. These changes can account for the strong decrease in serum triacylglycerol in rats fed a diet containing both sesamin and soybean phospholipid.

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Dietary Phospholipid-Dependent Reductions in Gene Expression and Activity of Liver Enzymes in Fatty Acid Synthesis in Fasted-Refed Rats.

Cited by 13 publications

References 29 publications

Dietary Phospholipids Ameliorate Fructose-Induced Hepatic Lipid and Metabolic Abnormalities in Rats

Dietary Phospholipids Ameliorate Fructose-Induced Hepatic Lipid and Metabolic Abnormalities in Rats

Conjugated Linoleic Acid (CLA) Inhibits Fatty Acid Synthetase Activityin Vitro

Combined effect of sesamin and soybean phospholipid on hepatic fatty acid metabolism in rats

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