Conjugated Linoleic Acid (CLA) Inhibits Fatty Acid Synthetase Activity<i>in Vitro</i>

Oku, Hirosuke; Wongtangtintharn, Sawitree; Iwasaki, Hiromichi; Toda, Takayoshi

doi:10.1271/bbb.67.1584

Cited by 21 publications

(13 citation statements)

References 16 publications

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“…It is possible that CLA could be affecting ACC phosphorylation and/or other enzymes of FA synthesis. For example, CLA supplementation decreased activity of other lipid synthesis enzymes including FA synthase in vitro (45) and hepatic microsomal phosphatidate phosphohydrolase in OLETF rats (10). The current study shows that CLA decreases liver ∆ 9 desaturase activity in both normal lean and obese insulin-resistant rats based on the FA composition of TAG and PL (Tables 4 and 5).…”

Section: Discussionsupporting

confidence: 50%

Conjugated linoleic acid reduces hepatic steatosis, improves liver function, and favorably modifies lipid metabolism in obese insulin‐resistant rats

Noto

Zahradka

Yurkova

et al. 2006

Lipids

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CLA has been shown to induce or suppress excess liver lipid accumulation in various animal models. Interestingly, the state of insulin resistance may be an important modulator of this effect. The objective of the current study was to determine how feeding a dietary CLA mixture would affect liver lipid accumulation in insulin-resistant/obese and lean rats in relation to liver function, lipidemia, liver TAG and phospholipid FA composition, and expression of hepatic markers of FA transport, oxidation, and synthesis. Six-week-old fa/fa and lean Zucker rats (n = 20/genotype) were fed either a 1.5% CLA mixture or a control diet for 8 wk. CLA supplementation reduced liver lipid concentration of fa/fa rats by 62% in concurrence with improved liver function (lower serum alanine aminotransferase and alkaline phosphatase) and favorable modification of the serum lipoprotein profile (reduced VLDL and LDL and elevated HDL) compared with controlfed fa/fa rats. The fa/fa genotype had two-thirds the amount of CLA (as % total FA) incorporated into liver TAG and phospholipids compared with the lean genotype. In both genotypes, CLA altered the hepatic FA profile (TAG greater than phospholipids) and these changes were explained by a desaturase enzyme index. Liver-FA-binding protein and acyl CoA oxidase, markers of FA transport and oxidation, respectively, were expressed at higher levels in CLA-fed fa/fa rats. In summary, these results illustrate a strong relationship between the state of insulin resistance and liver lipid metabolism and suggest that CLA acts to favorably modify lipid metabolism in fa/fa Zucker rats.

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Section: Discussionsupporting

confidence: 50%

Conjugated linoleic acid reduces hepatic steatosis, improves liver function, and favorably modifies lipid metabolism in obese insulin‐resistant rats

Noto

Zahradka

Yurkova

et al. 2006

Lipids

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“…CLA inhibited fatty acid synthetase activity in rat liver. (Oku et al, 2003), and the t10,c12 isomer attenuated lipogenesis in human AT cells (Brown et al, 2001). Therefore, CLA can affect lipid accumulation both by decreasing synthesis and increasing oxidation.…”

Section: Discussionmentioning

confidence: 97%

Influence of dietary conjugated linoleic acid (CLA) on lipid and fatty acid composition in liver and flesh of Atlantic salmon (Salmo salar)

Kennedy

Campbell

Porter

et al. 2005

Comparative Biochemistry and Physiology Part B: Biochemistry an

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The aim of the present study was to determine the effects of conjugated linoleic acid (CLA) on lipid and fatty acid metabolism in Atlantic salmon. The overall objective being to test the hypotheses that CLA has beneficial effects in salmon including growth enhancement, improved flesh quality through decreased adiposity and lipid deposition thereby minimising detrimental effects of feeding high fat diets, and increased nutritional quality through increased levels of beneficial fatty acids including n-3 highly unsaturated fatty acids (HUFA) and CLA itself. Salmon smolts were fed diets containing two levels of fish oil (low, ~18% and high, ~34%) containing three levels of CLA (a 1:1 mixture of 9-cis,trans-11 and trans-10,cis-12. at 0, 1 and 2% of diet) for 3 months and the effects on growth performance, liver and muscle (flesh) lipid contents and class compositions, and fatty acid compositions determined. The diets were also specifically formulated to investigate whether the effects of CLA, if any, were more dependent upon absolute content of CLA in the diet (as percentage of total diet) or the relative level of CLA to other fatty acids. Dietary CLA in salmon smolts had no effect on growth parameters or biometric parameters. However, there was a clear trend of increased total lipid and triacylglycerol contents in both liver and flesh in fish fed CLA, particularly in fish fed the high oil diets. Finally, CLA was incorporated into tissue lipids, with levels in flesh being 2-fold higher than in liver, but importantly, incorporation in liver was at the expense of saturated and monounsaturated fatty acids whereas in flesh it was at the expense of n-3HUFA.3

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“…A recent study showed that among the total 14 fatty acids examined, c9, t11 CLA exhibited the greatest inhibitory effect on liver FAS activity, followed by t10, c12 CLA. DHA was also shown to inhibit FAS activity but to a lesser extent than CLA [92]. In the study conducted by Tsuboyama-Kasaoka et al [21], the mRNA level of FAS showed marked decrease in female C57BL/6J mice after 5 months of supplementation with 1% CLA.…”

Section: Pufa Decrease Lipid Synthesis Throughmentioning

confidence: 92%

Anti‐obesity effects of conjugated linoleic acid, docosahexaenoic acid, and eicosapentaenoic acid

Huang²,

Xie³

2008

Molecular Nutrition Food Res

122

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Obesity has become a prevailing epidemic throughout the globe. Effective therapies for obesity become attracting. Food components with beneficial effects on "weight loss" have caught increasing attentions. Conjugated linoleic acid (CLA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) belong to different families of polyunsaturated fatty acids (PUFA). However, they have similar effects on alleviating obesity and/or preventing from obesity. They influence the balance between energy intake and expenditure; and reduce body weight and/or fat deposition in animal models, but show little effect in healthy human subjects. They inhibit key enzymes responsible for lipid synthesis, such as fatty acid synthase and stearoyl-CoA desaturase-1, enhance lipid oxidation and thermogenesis, and prevent free fatty acids from entering adipocytes for lipogenesis. PUFA also exert suppressive effects on several key factors involved in adipocyte differentiation and fat storage. Despite their similar effects and shared mechanisms, they display differences in the regulation of lipid metabolism. Moreover, DHA and EPA exhibit "anti-obesity" effect as well as improving insulin sensitivity, while CLA induces insulin resistance and fatty liver in most cases. A deeper and more detailed investigation into the complex network of anti-obesity regulatory pathways by different PUFA will improve our understanding of the mechanisms of body weight control and reduce the prevalence of obesity.

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Conjugated Linoleic Acid (CLA) Inhibits Fatty Acid Synthetase Activityin Vitro

Cited by 21 publications

References 16 publications

Conjugated linoleic acid reduces hepatic steatosis, improves liver function, and favorably modifies lipid metabolism in obese insulin‐resistant rats

Conjugated linoleic acid reduces hepatic steatosis, improves liver function, and favorably modifies lipid metabolism in obese insulin‐resistant rats

Influence of dietary conjugated linoleic acid (CLA) on lipid and fatty acid composition in liver and flesh of Atlantic salmon (Salmo salar)

Anti‐obesity effects of conjugated linoleic acid, docosahexaenoic acid, and eicosapentaenoic acid

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