Dietary pea fiber increases diversity of colonic methanogens of pigs with a shift from Methanobrevibacter to Methanomassiliicoccus-like genus and change in numbers of three hydrogenotrophs

Luo, Yuheng; Chen, Hong; Yu, Bing; He, Jun; Zheng, Ping; Mao, Xiangbing; Tian, Gang; Yu, Jie; Huang, Zhiqing; Luo, Junqiu; Chen, Shuai

doi:10.1186/s12866-016-0919-9

Cited by 25 publications

(25 citation statements)

References 48 publications

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“…The presence of this genus in rumen microbial communities is well known ( Henderson et al, 2013 ; Patra et al, 2017 ). Moreover, previous studies have also described its presence in the gastrointestinal tract of humans ( Thomas et al, 2017 ) and monogastric animals ( Hou et al, 2016 ; Luo et al, 2017 ); including rabbit as Kušar and Avguštin (2010) reported in their study. Nonetheless, Mi et al (2018) revealed the low presence of methanogenic archaea compared to Bacteria domain in rabbit cecum, due to its acidic pH (≈5.8) which does not favor the growth of methanogenic archaea.…”

Section: Discussionmentioning

confidence: 69%

Rabbit Microbiota Changes Throughout the Intestinal Tract

et al. 2018

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To gain insight into the importance of carefully selecting the sampling area for intestinal microbiota studies, cecal and fecal microbial communities of Caldes meat rabbit were characterized. The animals involved in the study were divided in two groups according to the feed intake level they received during the fattening period; ad libitum (n = 10) or restricted to 75% of ad libitum intake (n = 11). Cecum and internal hard feces were sampled from sacrificed animals. Assessment of bacterial and archaeal populations was performed by means of Illumina sequencing of 16S rRNA gene amplicons in a MiSeq platform. A total of 596 operational taxonomic units (OTUs) were detected using QIIME software. Taxonomic assignment revealed that microbial diversity was dominated by phyla Firmicutes (76.42%), Tenericutes (7.83%), and Bacteroidetes (7.42%); kingdom Archaea was presented at low percentage (0.61%). No significant differences were detected between sampling origins in microbial diversity or richness assessed using two alpha-diversity indexes: Shannon and the observed number of OTUs. However, the analysis of variance at genus level revealed a higher presence of genera Clostridium, Anaerofustis, Blautia, Akkermansia, rc4-4, and Bacteroides in cecal samples. By contrast, genera Oscillospira and Coprococcus were found to be overrepresented in feces, suggesting that bacterial species of these genera would act as fermenters at the end of feed digestion process. At the lowest taxonomic level, 83 and 97 OTUs in feces and cecum, respectively, were differentially represented. Multivariate statistical assessment revealed that sparse partial least squares discriminant analysis (sPLS-DA) was the best approach for this purpose. Interestingly, the majority of the most discriminative OTUs selected by sPLS-DA were found to be differentially represented between sampling origins in univariate analysis. Our study provides evidence that the choice of intestinal sampling area is relevant due to important differences in some taxa’s relative abundance that have been revealed between rabbits’ cecal and fecal microbiota. An appropriate sampling intestinal area should be chosen in each microbiota assessment.

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Section: Discussionmentioning

confidence: 69%

Rabbit Microbiota Changes Throughout the Intestinal Tract

et al. 2018

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“…Methanogens diversity in piglets is yet poorly understood. One metagenomic study performed in growing pig mid colon revealed that archaeal species are influenced by diet composition [51]. Additionally, the unique study performed using PCR-DGGE analysis on the fecal archaeal microbiota of weaning piglets revealed a shift from the Methanobrevibacter boviskoreani to M. smithii species during weaning transition [52].…”

Section: Discussionmentioning

confidence: 99%

“…The methanogenic archaeal community represent microbiota keystone species by transforming end-product from bacterial fermentation such as hydrogen, carbon dioxide or acetate into methane and potentially influencing the overall gut microbial populations [50,51]. In feces, the Methanobrevibacter genera performs hydrogenotrophic methanogenesis and transforms hydrogen and carbon dioxide derived from bacterial fermentations into methane and H 2 O [52].…”

Section: Discussionmentioning

confidence: 99%

Microbiota Composition and Functional Profiling Throughout the Gastrointestinal Tract of Commercial Weaning Piglets

et al. 2019

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Dietary, environmental, and social stresses induced by weaning transition in pig production are associated with alterations of gut microbiota, diarrhea, and enteric infections. With the boom of -omic technologies, numerous studies have investigated the dynamics of fecal bacterial communities of piglets throughout weaning but much less research has been focused on the composition and functional properties of microbial communities inhabiting other gastrointestinal segments. The objective of the present study was to bring additional information about the piglet bacterial and archaeal microbiota throughout the entire digestive tract, both at the structural level by using quantitative PCR and high-throughput sequencing, and on functionality by measurement of short-chain fatty acids and predictions using Tax4Fun tool. Our results highlighted strong structural and functional differences between microbial communities inhabiting the fore and the lower gut as well as a quantitatively important archaeal community in the hindgut. The presence of opportunistic pathogens was also noticed throughout the entire digestive tract and could trigger infection emergence. Understanding the role of the intestinal piglet microbiota at weaning could provide further information about the etiology of post-weaning infections and lead to the development of effective preventive solutions.

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“… Danielsson et al (2017) reported that unclassified Methanomassiliicoccaceae was 1.5-fold more abundant in low CH 4 emitters than that in high CH 4 emitters. Luo et al (2017) reported that dietary pea fiber increased the diversity of the colonic methanogen community structure of pigs with a shift from Methanobrevibacter to Methanomassiliicoccus and Methanomassiliicoccus -like genus. Hydrogenotrophic pathway, methylotrophic pathway and acetoclastic pathway are the three major pathways of methanogenesis.…”

Section: Discussionmentioning

confidence: 99%

Shifts of Hydrogen Metabolism From Methanogenesis to Propionate Production in Response to Replacement of Forage Fiber With Non-forage Fiber Sources in Diets in vitro

Wang

Nan

Chu³

et al. 2018

Front. Microbiol.

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The rumen microbial complex adaptive mechanism invalidates various methane (CH4) mitigation strategies. Shifting the hydrogen flow toward alternative electron acceptors, such as propionate, was considered to be a meaningful mitigation strategy. A completely randomized design was applied in in vitro incubation to investigate the effects of replacing forage fiber with non-forage fiber sources (NFFS) in diets on methanogenesis, hydrogen metabolism, propionate production and the methanogenic and bacterial community. There are two treatments in the current study, CON (a basic total mixed ration) and TRT (a modified total mixed ration). The dietary treatments were achieved by partly replacing forage fiber with NFFS (wheat bran and soybean hull) to decrease forage neutral detergent fiber (fNDF) content from 24.0 to 15.8%, with the composition and inclusion rate of other dietary ingredients remaining the same in total mixed rations. The concentrations of CH4, hydrogen (H2) and volatile fatty acids were determined using a gas chromatograph. The archaeal and bacterial 16S rRNA genes were sequenced by Miseq high-throughput sequencing and used to reveal the relative abundance of methanogenic and bacterial communities. The results revealed that the concentration of propionate was significantly increased, while the concentration of acetate and the acetate to propionate ratio were not affected by treatments. Compared with CON, the production of H2 increased by 8.45% and the production of CH4 decreased by 14.06%. The relative abundance of Methanomassiliicoccus was significantly increased, but the relative abundance of Methanobrevibacter tended to decrease in TRT group. At the bacterial phylum level, the TRT group significantly decreased the relative abundance of Firmicutes and tended to increase the relative abundance of Bacteroidetes. The replacement of forage fiber with NFFS in diets can affect methanogenesis by shifting the hydrogen flow toward propionate, and part is directed to H2 in vitro. The shift was achieved by a substitution of Firmicutes by Bacteroidetes, another substitution of Methanobrevibacter by Methanomassiliicoccus. Theoretical predictions of displacements of H2 metabolism from methanogenesis to propionate production was supported by the dietary intervention in vitro.

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Dietary pea fiber increases diversity of colonic methanogens of pigs with a shift from Methanobrevibacter to Methanomassiliicoccus-like genus and change in numbers of three hydrogenotrophs

Cited by 25 publications

References 48 publications

Rabbit Microbiota Changes Throughout the Intestinal Tract

Rabbit Microbiota Changes Throughout the Intestinal Tract

Microbiota Composition and Functional Profiling Throughout the Gastrointestinal Tract of Commercial Weaning Piglets

Shifts of Hydrogen Metabolism From Methanogenesis to Propionate Production in Response to Replacement of Forage Fiber With Non-forage Fiber Sources in Diets in vitro

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