Dietary lipid levels could improve growth and intestinal microbiota of juvenile swimming crab, Portunus trituberculatus

Sun, Peng; Jin, Min; Ding, Liyun; Lu, You; Ma, Haijun; Yang, Yuan; Zhou, Qicun

doi:10.1016/j.aquaculture.2018.02.018

Cited by 67 publications

(52 citation statements)

References 90 publications

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“…Due to its economic value, the culture of P. trituberculatus is now expanding rapidly in China and aquaculture production of this crab reached 119 777 tons in 2017 (15) . Until now, studies have focused mainly on dietary macronutrient requirements, such as protein and lipid, and optimal dietary lipid levels for P. trituberculatus have been reported to range from 4•2 to 13•8 % (16)(17)(18)(19) . However, the relationship between dietary lipid level and the regulation of lipid uptake, anabolism and catabolism has not been studied in P. trituberculatus.…”

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Effects of dietary lipid level on growth, fatty acid profiles, antioxidant capacity and expression of genes involved in lipid metabolism in juvenile swimming crab, Portunus trituberculatus

et al. 2019

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The regulation of lipogenesis and lipolysis mechanisms related to consumption of lipid has not been studied in swimming crab. The aims of the present study were to evaluate the effects of dietary lipid levels on growth, enzymes activities and expression of genes of lipid metabolism in hepatopancreas of juvenile swimming crab. Three isonitrogenous diets were formulated to contain crude lipid levels at 5·8, 9·9 and 15·1 %. Crabs fed the diet containing 15·1 % lipid had significantly lower growth performance and feed utilisation than those fed the 5·8 and 9·9 % lipid diets. Crabs fed 5·8 % lipid had lower malondialdehyde concentrations in the haemolymph and hepatopancreas than those fed the other diets. Highest glutathione peroxidase in haemolymph and superoxide dismutase in hepatopancreas were observed in crabs fed 5·8 % lipid. The lowest fatty acid synthase and glucose 6-phosphate dehydrogenase activities in hepatopancreas were observed in crabs fed 15·1 % lipid, whereas crabs fed 5·8 % lipid had lower carnitine palmitoyltransferase-1 activity than those fed the other diets. Crabs fed 15·1 % lipid showed lower hepatopancreas expression of genes involved in long-chain-PUFA biosynthesis, lipoprotein clearance, fatty acid uptake, fatty acid oxidation, lipid anabolism and lipid catabolism than those fed the other diets, whereas expression of some genes of lipoprotein assembly and fatty acid oxidation was up-regulated compared with crabs fed 5·8 % lipid. Overall, high dietary lipid level can inhibit growth, reduce antioxidant enzyme activities and influence lipid metabolic pathways to regulate lipid deposition in crab.

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Effects of dietary lipid level on growth, fatty acid profiles, antioxidant capacity and expression of genes involved in lipid metabolism in juvenile swimming crab, Portunus trituberculatus

et al. 2019

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“…Compared with mammals and birds, fish have lower carbohydrate utilization abilities, especially carnivorous fish (Weber & Haman, 1996;Wilson, 1994). Numerous studies have shown the protein-sparing potential of non-protein components (lipids and carbohydrates) in diets (Sun et al, 2018;Zhang et al, 2016;P. Zhou et al, 2016).…”

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confidence: 99%

Effects of different dietary carbohydrate‐to‐lipid ratios on growth, plasma biochemical indexes, digestive and immune enzymes activities of juvenile orange‐spotted grouper Epinephelus coioides

et al. 2020

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An 8‐week feeding trial was conducted to investigate the effects of dietary carbohydrate‐to‐lipid (CHO:L) ratios on growth, liver and muscle glycogen content, haematological indices, and liver and intestinal enzyme activity of juvenile grouper (Epinephelus coioides). Five isonitrogenous (496.0 g/kg protein) and isoenergetic (21.6 KJ/g gross energy) diets with varying CHO:L ratios of 0.65 (D1), 1.31 (D2), 2.33 (D3), 4.24 (D4) and 8.51 (D5), respectively, were fed to triplicate groups of 20 fish (average 10.02 ± 0.1 g) for 8 weeks. Results showed that the weight gain rate, specific growth rate and protein efficiency ratio (PER) of juvenile grouper increased first and then decreased with the increase in CHO:L ratio, reaching a maximum value in the D4 (CHO:L = 4.24) diet. The trend for the feed conversion ratio was opposite to the PER. Along with the diet CHO:L ratios, the apparent digestibility coefficients (ADC) of crude lipid and energy for the juvenile groupers decreased gradually, while the ADC of dry matter, liver and muscle glycogen level increased gradually. Total protein, triglycerides and cholesterol in serum were all maximized in the D4 diet and glucose in the D5 (CHO:L = 8.51) diet. Digestive enzyme activity in the intestine was significantly affected by dietary CHO:L ratio. Liver hexokinase, phosphofructokinase and glucose‐6‐phosphate dehydrogenase activity increased significantly as CHO:L ratio increased. Liver lysozyme and acid phosphatase activity in the groupers fed the D3 (CHO:L = 2.33) diet was significantly higher than that of other diets. Liver fructose‐1,6‐bisphosphatase and alkaline phosphatase activity reached a maximum value in the D4 diet and was significantly higher than that in the D1 diet. Taking the above results together, it can be concluded that an optimal dietary CHO:L ratio of 2.33 is suitable for grouper culture concerning growth performance and health.

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“…In turn, studies have shown that major nutrients can affect the composition of gut microbial communities such as carbohydrate (Pedrotti et al, ; Qiao et al, ; Ringø, Sperstad, Kraugerud, & Krogahl, ), lipid (Lødemel et al, ; Zhang et al, ) and protein (Hartviksen et al, ; Heikkinen et al, ; Ringø, Sperstad, Myklebust, Refstie, & Krogdahl, ). Over the past decade, there has been a noticeable increase in the researches of intestinal microbiota for its effect on crustaceans such as Eriocheir sinensis (Li et al, ; Zhang et al, ), Fenneropenaeus chinensis (Liu et al, ), Litopenaeus vannamei (Moss, Lea Master, & Sweeney, ; Suo et al, ; Zhang et al, ; Zhou et al, ), Macrobrachium rosenbergii (Mente, Gannon, Nikouli, Hammer, & Kormas, ), Penaeus monodon (Rungrassamee et al, ), Portunus trituberculatus (Sun et al, ) and Scylla paramamosain (Li, Sun, Wu, Hu, & Liu, ). However, few researches have reported the effects of dietary copper sources on intestinal microbiota in Pacific white shrimp, only Zhou et al ().…”

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confidence: 99%

Effects of different dietary copper sources on the growth and intestinal microbial communities of Pacific white shrimp ( Litopenaeus vannamei )

et al. 2019

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An 8‐week feeding trial was conducted to evaluate the effects of dietary copper sources on growth performance and intestinal microbial communities of juvenile Pacific white shrimp (Litopenaeus vannamei). Four isonitrogenous (420 g/kg crude protein) and isolipidic (70 g/kg crude lipid) experimental diets were formulated to contain different copper sources, Diet NSC (no‐supplemented copper), Diet CS (copper sulphate), Diet Availa (copper amino acid complex from Availa®Cu100) and Diet M (1:1, copper sulphate + copper amino acid complex). A total of 360 Pacific white shrimp juveniles (initial weight: 1.86 ± 0.03 g) were randomly allocated into four groups, with three replicates per group and 30 shrimps each replicate. An Illumina‐based sequencing method was used to analyse the intestinal bacterial composition of shrimp juveniles. The results revealed that shrimps fed Diet M had significantly higher percent weight gain (PWG) than that fed Diet NSC and Diet CS, while there were no significant differences between Diet M and Diet Availa. Survival was not affected by the dietary copper sources. Proteobacteria and Bacteroidetes were dominant in the intestines of Pacific white shrimp. Shrimps fed Diet M and Diet Availa had higher bacterial richness and diversity than those fed Diet NSC and Diet CS with no significant differences among all treatments. Furthermore, Diet M and Diet Availa which contained copper amino acid complex reduced the relative abundance of Vibrionaceae in shrimp intestinal tract. This study indicated that dietary copper sources could affect the intestinal microbial communities and diets supplemented with Availa®Cu100 could have a positive impact on the growth performance and decrease the potential risk of disease in Pacific white shrimp.

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Dietary lipid levels could improve growth and intestinal microbiota of juvenile swimming crab, Portunus trituberculatus

Cited by 67 publications

References 90 publications

Effects of dietary lipid level on growth, fatty acid profiles, antioxidant capacity and expression of genes involved in lipid metabolism in juvenile swimming crab, Portunus trituberculatus

Effects of dietary lipid level on growth, fatty acid profiles, antioxidant capacity and expression of genes involved in lipid metabolism in juvenile swimming crab, Portunus trituberculatus

Effects of different dietary carbohydrate‐to‐lipid ratios on growth, plasma biochemical indexes, digestive and immune enzymes activities of juvenile orange‐spotted grouper Epinephelus coioides

Effects of different dietary copper sources on the growth and intestinal microbial communities of Pacific white shrimp ( Litopenaeus vannamei )

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