Dietary intake, plasma homocysteine, and repetitive element DNA methylation in the Multi-Ethnic Study of Atherosclerosis (MESA)

Perng, Wei; Villamor, Eduardo; Shroff, Monal R.; Nettleton, Jennifer A.; Pilsner, J. Richard; Liu, Y.; Diez‐Roux, Ana V.

doi:10.1016/j.numecd.2013.11.011

Cited by 40 publications

(34 citation statements)

References 29 publications

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“…The results of the present study showed that the methylation levels of these AS-associated genes were significantly correlated with the ratio of SAM/SAH in the ApoE -/-mice. In conclusion, FABP4, EC-SOD and MCP-1 are important in the formation and/or development of AS, and previous studies have confirmed that DNA methylation is a diagnostic biomarker of AS (51)(52)(53). On this basis, the present study analyzed the correlation between DNA methylation of FABP4, EC-SOD, MCP-1, and B1 repetitive elements and the serum SAM/SAH ratio to confirm the hypothesis that the serum SAM/SAH ratio may be a more sensitive biomarker of AS, and …”

Section: A B C Dsupporting

confidence: 59%

Ratio of S-adenosylmethionine to S-adenosylhomocysteine as a sensitive indicator of atherosclerosis

Zhang

Liu

et al. 2016

Mol Med Report

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Abstract. The present study aimed to confirm whether the ratio of S-adenosylmethionine (SAM) to S-adenosylhomocysteine (SAH) is a sensitive indicator, and whether it can be used as a biomarker for the clinical diagnosis of atherosclerosis. Apolipoprotein E (ApoE) -/-mice were randomly divided into four groups and fed with a high methionine diet for 15 weeks. Serum levels of homocysteine (Hcy) were measured using an automatic biochemistry analyzer. The concentrations of SAM and SAH were determined using high-performance liquid chromatography. The methylation levels of B1 repetitive elements, adipocyte fatty acid binding protein (FABP4), monocyte chemoattractant protein-1 (MCP-1) and extracellular superoxide dismutase (EC-SOD) were analyzed using nested touchdown-methylation-specific-polymerase chain reaction analysis. After 15 weeks, compared with the normal control group, serum concentrations of Hcy were significantly increased by 1.15-, 2.54-and 1.17-fold (P<0.05) in the ApoE -/-control group, Meth group and Meth-F group, respectively. The sizes of the atherosclerotic lesions were increased in the ApoE -/-control group, Meth group and Meth-F group, by up to 1.44-, 2.40-and 1.45-fold, respectively, compared with the normal control group (P<0.05). The concentrations of SAM were significantly increased by 3.02-, 3.42-and 2.46-fold in the ApoE -/-control group, Meth group and Meth-F group, respectively (P<0.05). The ratios of SAM/SAH were increased by 1.67-and 2.75-fold in the in ApoE -/-control group and Meth group, respectively, compared with the normal control group. The methylation levels of B1 repetitive elements, FABP4, MCP-1 and EC-SOD were decreased and exhibited hypomethylation. The methylation statuses of these genes were correlated with the ratio of the serum levels of SAM and SAH. These findings suggested that the SAM/SAH ratio is a biomarker and may provide a sensitive indicator for the clinical diagnosis of atherosclerosis.

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Section: A B C Dsupporting

confidence: 59%

Ratio of S-adenosylmethionine to S-adenosylhomocysteine as a sensitive indicator of atherosclerosis

Zhang

Liu

et al. 2016

Mol Med Report

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“…Despite these findings, few studies examined the association between LINE‐1 methylation and either obesity or weight loss. In one study, participants with BMI ≥40 kg/m 2 had higher levels of LINE‐1 methylation than participants with a BMI ≤25 . As our study only examined women with an initial BMI ≥25 kg/m 2 , there was insufficient variation to make a comparable analysis.…”

Section: Discussionmentioning

confidence: 94%

No effect of weight loss on LINE‐1 methylation levels in peripheral blood leukocytes from postmenopausal overweight women

Duggan

Xiao

Terry

et al. 2014

Obesity

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Objectives Obesity and weight-loss are associated with methylation patterns in specific genes, but their effect on Long Interspersed Nuclear Elements (LINE-1) methylation, a measure of global methylation is largely unknown. Methods Three hundred overweight/obese post-menopausal women (50–75 years) were part of a completed, 1-year randomized controlled trial, comparing independent and combined effects of a reduced-calorie weight-loss diet, and exercise program, vs. control. DNA was extracted from peripheral blood leukocytes collected at baseline and 12-months, and LINE-1 methylation analyzed by pyrosequencing. We compared mean changes between groups using generalized estimating equations and examined effects of weight-loss on LINE-1 methylation using stratified analyses (gained weight/no weight-loss (N=84); <5% (N=45); 5–10% (N=45); >10% of baseline weight-loss (N=126)) within each arm, adjusted by blood cell counts. We also examined associations between LINE-1 methylation and previously measured biomarkers, and anthropometrics. Results No significant difference in LINE-1 methylation levels was detected in any intervention group vs. controls. The magnitude of weight-loss was not associated with LINE-1 methylation at 12-months. There were no associations between baseline characteristics of participants, or previously measured biomarkers, and LINE-1 methylation. Conclusions Our results suggest that lifestyle changes sufficient to significantly reduce weight over 12-months may not change LINE-1 DNA methylation levels.

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“…Furthermore, recent studies have demonstrated a relationship between DNA methylation and metabolic diseases, such as obesity [21]. Therefore, the methylation levels of the promoter region in the following genes IL6 , LINE1 , SERPINE1, and TNF , previously linked with this type of pathological conditions [21,31,32,33,37,38,39], were evaluated in obese subjects with SAHS, in order to examine the molecular links between resting oxygen consumption and chronic diseases. Methylation levels of IL6 and SERPINE1 were positively associated with FFM.…”

Section: Discussionmentioning

confidence: 99%

“…Several polymerase chain reaction (PCR)-based methods have been used in order to easily analyze DNA methylation from leukocytes. In this context, global DNA methylation has been assessed measuring the methylation of CpG islands in the promoter region of the long interspersed nucleotide element 1 ( LINE1 ) retrotransposon [30,31,32,33], the only active, autonomous transposable element in humans [34]. Besides, some studies have investigated the relation between methylation pattern of genes with obesity, metabolic disturbances, and aging [27,28,35,36].…”

Section: Introductionmentioning

confidence: 99%

Low Oxygen Consumption is Related to a Hypomethylation and an Increased Secretion of IL-6 in Obese Subjects with Sleep Apnea-Hypopnea Syndrome

Lopez‐Pascual

Lasa²,

Portillo³

et al. 2017

Ann Nutr Metab

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Background: Deoxyribonucleic acid (DNA) methylation is an epigenetic modification involved in gene expression regulation, usually via gene silencing, which contributes to the risks of many multifactorial diseases. The aim of the present study was to analyze the influence of resting oxygen consumption on global and gene DNA methylation as well as protein secretion of inflammatory markers in blood cells from obese subjects with sleep apnea-hypopnea syndrome (SAHS). Methods: A total of 44 obese participants with SAHS were categorized in 2 groups according to their resting oxygen consumption. DNA methylation levels were evaluated using a methylation-sensitive high resolution melting approach. Results: The analyzed interleukin 6 (IL6) gene cytosine phosphate guanine (CpG) islands showed a hypomethylation, while serum IL-6 was higher in the low compared to the high oxygen consumption group (p < 0.05). Moreover, an age-related loss of DNA methylation of tumor necrosis factor (B = -0.82, 95% CI -1.33 to -0.30) and long interspersed nucleotide element 1 (B = -0.46; 95% CI -0.87 to -0.04) gene CpGs were found. Finally, studied CpG methylation levels of serpin peptidase inhibitor, clade E member 1 (r = 0.43; p = 0.01), and IL6 (r = 0.41; p = 0.02) were positively associated with fat-free mass. Conclusions: These findings suggest a potential role of oxygen in the regulation of inflammatory genes. Oxygen consumption measurement at rest could be proposed as a clinical biomarker of metabolic health.

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Dietary intake, plasma homocysteine, and repetitive element DNA methylation in the Multi-Ethnic Study of Atherosclerosis (MESA)

Cited by 40 publications

References 29 publications

Ratio of S-adenosylmethionine to S-adenosylhomocysteine as a sensitive indicator of atherosclerosis

Ratio of S-adenosylmethionine to S-adenosylhomocysteine as a sensitive indicator of atherosclerosis

No effect of weight loss on LINE‐1 methylation levels in peripheral blood leukocytes from postmenopausal overweight women

Low Oxygen Consumption is Related to a Hypomethylation and an Increased Secretion of IL-6 in Obese Subjects with Sleep Apnea-Hypopnea Syndrome

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