Dietary fats alter the activity and expression of glucose‐6‐phosphate dehydrogenase in rat lymphoid cells and tissues

Otton, Rosemari; Graziola, Fabiana; Hirata, Mário H.; Curi, Rui; Williams, John F.

doi:10.1080/15216549800204052

Cited by 11 publications

(11 citation statements)

References 5 publications

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“…For the enzymatic activities and oxidative lesions in biomolecules cells were pelleted (5 × 10 6 ) and mixed with 1.0 mL of the assayspecific extraction buffer, vortexed briefly and lysed by ultrasonication in a Vibra Cell apparatus (Connecticut, USA) as previously described [25]. A centrifugation step was included (10,000 ×g, 10 min, at 4°C) in order to eliminate debris from the crude homogenate; the supernatant was then used for further analysis.…”

Section: Preparation Of Homogenatesmentioning

confidence: 99%

ROS production in neutrophils from alloxan-induced diabetic rats treated in vivo with astaxanthin

Marin

Bolin

Macedo

et al. 2011

International Immunopharmacology

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Section: Preparation Of Homogenatesmentioning

confidence: 99%

ROS production in neutrophils from alloxan-induced diabetic rats treated in vivo with astaxanthin

Marin

Bolin

Macedo

et al. 2011

International Immunopharmacology

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“…17 A centrifugation step was included (10 000 Âg, 10 min, at 48C) in order to eliminate debris from the crude homogenate; the supernatant was then used for further analysis. The extracts for enzyme assays and oxidative lesions were prepared with a 50 mM phosphate buffer (pH 7.4).…”

Section: Preparation Of Homogenatesmentioning

confidence: 99%

Testosterone suppresses oxidative stress in human neutrophils

Marin¹,

Bolin²,

Santos³

et al. 2010

Cell Biochemistry & Function

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The in vitro effect of testosterone on human neutrophil function was investigated. Blood neutrophils from healthy male subjects were isolated and treated with 10 nM, 0.1 and 10 microM testosterone for 24 h. As compared with untreated cells, the testosterone treatment produced a significant decrease of superoxide production as indicated by the measurement of extra- and intracellular superoxide content. An increment in the production of nitric oxide was observed at 0.1 and 10 microM testosterone concentrations, whereas no effect was found for 10 nM. Intracellular calcium mobilization was significantly increased at 10 nM, whereas it was reduced at 10 microM testosterone. There was an increase in phagocytic capacity at 10 nM and a decrease of microbicidal activity in neutrophils treated with testosterone at 10 microM. Glutathione reductase activity was increased by testosterone treatment, whereas no effect was observed in other antioxidant enzyme activities. An increase in the content of thiol groups was observed at all testosterone concentrations. Lipid peroxidation in neutrophils evaluated by levels of TBARS was decreased at 10 nM and 0.1 microM testosterone. These results indicate the antioxidant properties of testosterone in neutrophils as suggested by reduction of superoxide anion production, and lipid peroxidation, and by the increase in nitric oxide production, glutathione reductase activity and the content of thiol groups. Therefore, the plasma levels of testosterone are important regulators of neutrophil function and so of the inflammatory response.

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“…Glutathione peroxidase [23] and glutathione reductase [24] activities were measured based on the oxidation of β-NADPH in the presence of tert-butyl hydroperoxide used as substrate. The activity of glucose-6-phosphate dehydrogenase was determined as described in our previous study [25]. …”

Section: Erythrocytes Enzyme Activitiesmentioning

confidence: 99%

Cytokines and Oxidative Stress Status Following a Handball Game in Elite Male Players

Marin

Moreira

Macedo

et al. 2010

Free Radical Biology and Medicine

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Background.Handball is considered an intermittent sport that places an important stress on a player's aerobic and anaerobic metabolism. However, the oxidative stress responses following a handball game remain unknown. We investigated the responses of plasma and erythrocyte antioxidant system and oxidative stress biomarkers following a single handball game. Methods. Fourteen male elite Brazilian handball athletes were recruited in the present study. Blood samples were taken before, immediately, and 24 hours after the game. Results. After the game and during 24 hours of recovery, the concentration of all oxidative stress indices changed significantly in a way indicating increased oxidative stress in the blood (thiol groups and reduced glutathione decreased, whereas TBARS and plasma antioxidant capacity was increased) as well as in erythrocyte (increased levels of TBARS and protein carbonyls). Erythrocyte antioxidant enzyme activities were also significantly changed by handball. Muscle damage indices (creatine kinase and lactate dehydrogenase) increased significantly after exercise. In addition, IL-6 increased after the game, whereas TNF-α decreased during recovery. Conclusion. This study demonstrates that a single handball game in elite athletes induces a marked state of oxidative stress evidenced by the oxidative modification in plasma and erythrocyte macromolecules, as well as by changes in the enzymatic and nonenzymatic antioxidant system.

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Dietary fats alter the activity and expression of glucose‐6‐phosphate dehydrogenase in rat lymphoid cells and tissues

Cited by 11 publications

References 5 publications

ROS production in neutrophils from alloxan-induced diabetic rats treated in vivo with astaxanthin

ROS production in neutrophils from alloxan-induced diabetic rats treated in vivo with astaxanthin

Testosterone suppresses oxidative stress in human neutrophils

Cytokines and Oxidative Stress Status Following a Handball Game in Elite Male Players

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