Dietary Docosahexaenoic Acid Can Alter the Surface Expression of CD4 and CD8 on T Cells in Peripheral Blood

Sasaki, Taku; Kanke, Yusuke; Nagahashi, Masahito; Toyokawa, Maki; Matsuda, Michiyo; Shimizu, Jun; Misawa, Yoshihisa; Takita, Toshichika

doi:10.1021/jf990358i

Cited by 12 publications

(6 citation statements)

References 16 publications

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“…As a consequence, a detrimental effect may occur, because host defense against bacterial and other antigens could be compromised (33). Likewise, a significant reduction of the expression of CD4 or CD8 on cell surfaces has recently been documented after dietary supplementation with DHA (34). The present results have confirmed that some dietary lipids are related to a reduction of lymphocyte proliferation stimulated by ConA or LPS.…”

Section: Discussionsupporting

confidence: 78%

Relevance of Dietary Lipids as Modulators of Immune Functions in Cells Infected with Listeria monocytogenes

Puertollano

Pablo

Cienfuegos

2002

Clin Vaccine Immunol

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Nutritional status may have significant importance for the immune system, and particularly, unsaturated fatty acids may serve as modulators of immune functions. Clinical and epidemiological studies have demonstrated that fatty acids are involved in the reduction of the inflammatory processes that occur in diseases characterized by an overactivation of the immune system. At the same time, an increase in susceptibility to infection has also been reported. The importance of immune system modulation by dietary lipids in the presence of an intracellular bacterial pathogen, such as Listeria monocytogenes, was evaluated in the present study. BALB/c mice were divided into four groups which were each fed a low-fat (2.5% by weight) diet, an olive oil (OO; 20% by weight) diet, a fish oil (FO; 20% by weight) diet, or a hydrogenated coconut oil (HCO; 20% by weight) diet for 4 weeks. In each group, lymphocye proliferation was measured, and a reduction in the stimulation index was observed in the FO and HCO groups. Cytotoxicity exerted by L. monocytogenes was increased in the groups fed diets containing OO and FO after 6 h of incubation with the bacterium. An important increase in the production of reactive oxygen species was found in the groups fed the HCO diet after 12 h of incubation with L. monocytogenes. Finally, invasion and adhesion factors were not modified substantially by the action of dietary lipids, although these factors were reduced in cells from mice fed an FO diet. These results underline the importance of several dietary lipids as biological modulators of immune functions and their crucial role in the alteration of host natural resistance.According to present knowledge, many fatty acids contained in the diets of both animals and humans may exert a modulatory effect on immune functions (5, 12). It is generally assumed that the long-chain n-3 polyunsaturated fatty acids eicosapentaenoic acid (20:5[n-3]) and docosahexaenoic acid (DHA; 22: 6[n-3]), contained in fish oil, promote an immunosuppressive function. However, it has also been determined that monounsaturated fatty acids, such as oleic acid (derived from olive oil), rather than the other biological substances contained in this fat, cause an important reduction of numerous immune functions (21). Therefore, as a consequence of these events, many immunological functions, such as lymphocyte proliferation (15,24), cytokine production (15, 24), expression of cell surface molecules (20), natural killer (NK) cell activity (36), and phagocytosis (7), are modulated by the action of fatty acids. As a result, in recent years, numerous studies have indicated the potential role of several dietary lipids in the development of cancer or in the increase in resistance to tumors (9, 31). Similarly, unsaturated fatty acids have been applied in the resolution of inflammatory disorders associated with diseases characterized by an overreactivation of the immune system, such as rheumatoid arthritis (22, 23), psoriasis (2), and multiple sclerosis (1,18). However, despite the benefi...

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Section: Discussionsupporting

confidence: 78%

Relevance of Dietary Lipids as Modulators of Immune Functions in Cells Infected with Listeria monocytogenes

Puertollano

Pablo

Cienfuegos

2002

Clin Vaccine Immunol

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“…The number of lymphocytes CD4 + and CD8 + cells, and spleen mononuclear CD4 + , CD8 + and IgM + cells did not differ between treatment groups (Table 5). Similar results of no difference between layer birds fed n-3 and n-6 fatty acids have been reported (Sasaki et al 2000;Wang et al 2000). Kelley et al (1991) reported no change in human peripheral cells by feeding n-3 fatty acids.…”

Section: Immune Cells Phenotypingsupporting

confidence: 80%

“…The changes brought about in the immune response of layer birds fed CLA, SFA, n-6 and n-3 fatty acids cannot be explained based on CD4 + /CD8 + cell population. Different fatty acids impede intracellular interaction involved in antigen recognition by T cells and antigen presenting cells and influence cell-mediated and antibody-mediated response differently (Sasaki et al 2000). One other possibility is that the difference in the level of activation of CD4 + /CD8 + positive cells, without affecting their absolute numbers, could have caused this effect.…”

Section: Immune Cells Phenotypingmentioning

confidence: 99%

Changes in delayed type hypersensitivity, egg antibody content and immune cell fatty acid composition of layer birds fed conjugated linoleic acid, n-6 or n-3 fatty acids

Selvaraj¹,

Cherian²

2004

Can. J. Anim. Sci.

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The effects of polyunsaturated fatty acids on delayed type hypersensitivity (DTH), egg yolk antibody content, immune tissue fatty acid profile and lipid oxidation products of layer birds were investigated. One hundred and twenty layer birds were fed diets containing conjugated linoleic acid (CLA) + animal fat (Diet I), sunflower oil (Diet II), canola + flax oil (Diet III) or fish oil (Diet IV). The total added lipid content of the diet was 3%. Birds fed Diets III and IV had higher content of n-3 fatty acids in lymphocyte and splenocytes. Thiobarbituric reactive substances were higher (P < 0.05) in the breast and thigh muscle of Diet IV fed birds. Serum and yolk anti-BSA antibody contents were higher (P < 0.05) in birds fed Diets III and IV. DTH was decreased (P < 0.05) in birds fed Diets IV and III. The number of lymphocyte CD4+ and CD8+ cells and spleen mononuclear cell CD4+, CD8+ and IgM+ cells did not differ (P > 0.05) between treatment groups. Feeding n-3 fatty acids increased antibody-mediated immune response, while n-6 fatty acids and CLA increased cell-mediated immune response. Key words: Conjugated linoleic acid, polyunsaturated fatty acids, delayed type hypersensitivity, immunoglobulins

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“…The percentages of T‐lymphocyte subpopulations in the spleen were measured by flow cytometry analysis 11. Splenocyte suspensions (1 × 10 6 cells mL −1 ) were doubly labelled with fluorescein isothiocyanate (FITC)‐conjugated and phycoerythrin (PE)‐conjugated antibodies for 15 min at room temperature (18–25 °C).…”

Section: Methodsmentioning

confidence: 99%

Protective effect of a marine oligopeptide preparation from Chum Salmon (Oncorhynchus keta) on radiation‐induced immune suppression in mice

et al. 2010

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Dietary Docosahexaenoic Acid Can Alter the Surface Expression of CD4 and CD8 on T Cells in Peripheral Blood

Cited by 12 publications

References 16 publications

Relevance of Dietary Lipids as Modulators of Immune Functions in Cells Infected with Listeria monocytogenes

Relevance of Dietary Lipids as Modulators of Immune Functions in Cells Infected with Listeria monocytogenes

Changes in delayed type hypersensitivity, egg antibody content and immune cell fatty acid composition of layer birds fed conjugated linoleic acid, n-6 or n-3 fatty acids

Protective effect of a marine oligopeptide preparation from Chum Salmon (Oncorhynchus keta) on radiation‐induced immune suppression in mice

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