Dietary diisononylphthalate contamination induces hepatic stress: a multidisciplinary investigation in gilthead seabream (Sparus aurata) liver

Carnevali, Oliana; Santobuono, Martina; Forner-Piquer, Isabel; Randazzo, Basilio; Mylonas, Constantinos C.; Ancillai, Daniele; Giorgini, Elisabetta; Maradonna, Francesca

doi:10.1007/s00204-019-02494-7

Cited by 16 publications

(9 citation statements)

References 151 publications

(157 reference statements)

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“…Oxidative stress is one of the main causes of cellular injuries and the endogenous anti-oxidant defense can prevent ROS-mediated damage [46]. To elucidate the role of PD in the oxidative stress response, the expression of a set of mRNA codifying for proteins with a pivotal role in the organism antioxidant defense, superoxide dismutase (sod1 and sod2) and catalase (cat), were investigated [34,47] [48]. Owing to their fast diffusion and versatile biological activities, reactive oxygen species, including hydrogen peroxide (H 2 O 2 ), are interesting candidates for wound-to-leukocyte signaling.…”

Section: Discussionmentioning

confidence: 99%

“…Catalase activity was performed as previously described in [34]. Zebrafish samples were prepared as described in [42].…”

Section: Catalase Activitymentioning

confidence: 99%

“…PD beneficial effects were investigated both before and after CuSO 4 exposure, in order to gain knowledge, in the first case, of eventual PD protective effects and in the second one, of its capacity to heal Cu toxicity. Response to oxidative stress was analyzed by evaluating the expression of genes codifying for superoxide dismutases (sod1 and sod2), and catalase (cat) mRNA and related activity [34], all central endogenous anti-oxidant enzymes involved in the first-line defense against ROS [6]. In addition, to get stronger evidence regarding PD anti-inflammatory effects, the amputation of the tail was also performed and the onset of inflammation was checked in the different experimental groups.…”

Section: Introductionmentioning

confidence: 99%

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Polydatin Beneficial Effects in Zebrafish Larvae Undergoing Multiple Stress Types

Pessina

Vincenzo

Maradonna

et al. 2021

IJERPH

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Polydatin is a polyphenol, whose beneficial properties, including anti-inflammatory and antioxidant activity, have been largely demonstrated. At the same time, copper has an important role in the correct organism homeostasis and alteration of its concentration can induce oxidative stress. In this study, the efficacy of polydatin to counteract the stress induced by CuSO4 exposure or by caudal fin amputation was investigated in zebrafish larvae. The study revealed that polydatin can reduced the stress induced by a 2 h exposure to 10 µM CuSO4 by lowering the levels of il1b and cxcl8b.1 and reducing neutrophils migration in the head and along the lateral line. Similarly, polydatin administration reduced the number of neutrophils in the area of fin cut. In addition, polydatin upregulates the expression of sod1 mRNA and CAT activity, both involved in the antioxidant response. Most of the results obtained in this study support the working hypothesis that polydatin administration can modulate stress response and its action is more effective in mitigating the effects rather than in preventing chemical damages.

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Section: Discussionmentioning

confidence: 99%

“…Catalase activity was performed as previously described in [34]. Zebrafish samples were prepared as described in [42].…”

Section: Catalase Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polydatin Beneficial Effects in Zebrafish Larvae Undergoing Multiple Stress Types

Pessina

Vincenzo

Maradonna

et al. 2021

IJERPH

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“…Kang et al showed that DINP contributes to the development of allergic asthma by promoting the elevation of oxidative stress and activating the NF-Kβ signaling pathway. The toxic effects of DINP such as acute toxicity, mutagenicity, carcinogenicity, and reproductive and developmental toxicity − have been associated with ROS accumulation. For instance, Ma et al demonstrated that the hepatic injury in mice was caused by the toxic effect of ROS accumulation by DINP.…”

Section: Discussionmentioning

confidence: 99%

Diisononyl Phthalate Differentially Affects Sirtuin Expression in the HepG2 Cell Line

Gutiérrez-García

Choudhury

León‐Rodríguez

2019

Chem. Res. Toxicol.

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Human exposure to phthalates has received special attention due to their possible adverse human health effects. Diisononyl phthalate (DINP) is a plasticizer still widely used in many products, despite being considered an endocrine disruptor. In this study, we evaluated DINP's cytotoxicity, its effect on the levels of reactive oxygen species (ROS), and its effect on sirtuin expression in HepG2 cells. Results showed that 1 μg/mL DINP significantly downregulated Sirt1, Sirt2, Sirt3, and Sirt5 gene expression (p < 0.05), while other sirtuins remained unaffected. Furthermore, protein levels of Sirt1 and Sirt3 were significantly downregulated by 1 μg/mL DINP. On the other hand, 100 μg/mL DINP doubled the levels of lysine acetylation proteins (increased 2-fold) as well as reactive oxygen species (ROS) compared with the controls. In conclusion, our study suggests, for the first time, that DINP regulates the potential epigenetic disruptor sirtuin family and leads to induction of ROS via sirtuins.

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“…However, as a major DEHP substitute, DINP has received much less attention with regard to its potential impacts on lipid metabolism, particularly in mammals. To the best of our knowledge, the only data available were reported in aquatic organisms including zebrafish and seabream studies, which indicated that exposure to DINP causes disturbances in lipid-related metabolism, as well as the induction of oxidative stress and activation of immune responses. − …”

Section: Introductionmentioning

confidence: 99%

DEHP and DINP Induce Tissue- and Gender-Specific Disturbances in Fatty Acid and Lipidomic Profiles in Neonatal Mice: A Comparative Study

Huang

Sun

Tan

et al. 2019

Environ. Sci. Technol.

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Di-isononyl phthalate (DINP) is considered one of the main industrial alternatives to di(2-ethylhexyl)phthalate (DEHP), a well-known chemical with various toxic effects including the disruption with lipid metabolism. However, the potential effects of DINP on lipid metabolism have rarely been investigated in mammals. Our study demonstrated that exposure of neonatal mice to DEHP and DINP at a daily dose of 0.048 or 4.8 mg/kg from postnatal day 0 (PND0) to PND21 caused nonmonotonic as well as tissue- and gender-specific alterations of total fatty acid (FA) compositions in plasma, heart, and adipose tissues. However, the patterns of disruption differed between DEHP- and DINP-treated groups. On the basis of targeted lipidomic analyses, we further identified gender-specific alterations of eight lipid classes in plasma following DEHP or DINP exposure. At the higher dose, DEHP induced decreases in total phosphatidylcholines and phosphatidylinositol (PI) in females and increases in phosphatidylethanolamines (PEs) and triglycerides in males. By contrast, DINP at the higher dose caused alterations of PEs, PIs, phosphatidylserines, and cholesterols exclusively in male mice, but no changes were observed in female pups. Although the most significant dysregulation of lipid metabolism was often observed for the higher dose, the lower one could also disrupt lipid profiles and sometimes its effects may even be more significant than those induced by the higher dose. Our study for the first time identified tissue- and gender-specific disruptions of FA compositions and lipidomic profiles in mice neonatally exposed to DINP. These findings question the suitability of DINP as a safe DEHP substitute and lay a solid foundation for further elucidation of its effects on lipid metabolism and underlying mechanisms.

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Dietary diisononylphthalate contamination induces hepatic stress: a multidisciplinary investigation in gilthead seabream (Sparus aurata) liver

Cited by 16 publications

References 151 publications

Polydatin Beneficial Effects in Zebrafish Larvae Undergoing Multiple Stress Types

Polydatin Beneficial Effects in Zebrafish Larvae Undergoing Multiple Stress Types

Diisononyl Phthalate Differentially Affects Sirtuin Expression in the HepG2 Cell Line

DEHP and DINP Induce Tissue- and Gender-Specific Disturbances in Fatty Acid and Lipidomic Profiles in Neonatal Mice: A Comparative Study

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