Polydatin Beneficial Effects in Zebrafish Larvae Undergoing Multiple Stress Types

Abstract

Polydatin is a polyphenol, whose beneficial properties, including anti-inflammatory and antioxidant activity, have been largely demonstrated. At the same time, copper has an important role in the correct organism homeostasis and alteration of its concentration can induce oxidative stress. In this study, the efficacy of polydatin to counteract the stress induced by CuSO4 exposure or by caudal fin amputation was investigated in zebrafish larvae. The study revealed that polydatin can reduced the stress induced by a 2 h exposure to 10 µM CuSO4 by lowering the levels of il1b and cxcl8b.1 and reducing neutrophils migration in the head and along the lateral line. Similarly, polydatin administration reduced the number of neutrophils in the area of fin cut. In addition, polydatin upregulates the expression of sod1 mRNA and CAT activity, both involved in the antioxidant response. Most of the results obtained in this study support the working hypothesis that polydatin administration can modulate stress response and its action is more effective in mitigating the effects rather than in preventing chemical damages.

Polydatin: Pharmacological Mechanisms, Therapeutic Targets, Biological Activities, and Health Benefits

“…Diabetes mellitus during myocardial infarction/reperfusion (MI/R) can increase the severity of oxidative/nitrative injury. Polydatin reduced MI/R cardiac damage during diabetes by activating the Notch1/Hes1-dependent Pten/Akt signaling pathway [ 36 , 66 , 123 ]. Assessment of the effects of polydatin on diabetes-associated myocardial hypertrophy showed a decrease in fasting blood glucose, insulin, HbA1C, weight gain, and insulin resistance levels by inhibiting NF-κB, COX-2, and the iNOS signaling pathway and activating PPARβ.…”