Dietary Deprivation of B-Vitamins Reflected in Murine Splenocyte Proliferation in Vitro

Matthews, Kathleen S.; Mrowczynski, Emilia; Matthews, R.

doi:10.1006/bbrc.1994.1066

“…Additional, but nontoxic, amounts of retinyl palmitate have been shown to lower the susceptibility of rats to salmonella infection in vivo by enhancing phagocytic functions of isolated Kupffer cells, peritoneal and splenic macrophages (Hatchigian et al 1989). Dietary deprivation of B vitamins, and especially that of riboflavin, significantly lowers the macrophage counts in murine spleen (Matthews et al 1994). Treatment of monocytic human cell lines with cholecalciferol in vitro increases their phagocytic activity and enhances their oxidative burst capacity (Hauck et al 1997).…”

Section: Vitamins: Reactive Oxygen Species: Chemiluminescence: Respirmentioning

confidence: 99%

Effect of multivitamins in an effervescent preparation on the respiratory burst of peritoneal macrophages in mice

Jakus

¹

,

Kriska

²

,

Vanyúr

³

2002

British Journal of Nutrition

1

0

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The effect of regular intake of low doses of an effervescent multivitamin preparation on the free-radical-producing activity of murine peritoneal macrophages under conditions resembling a possible infection was studied in vitro. Initially, several groups of mice were fed a basal diet and given, for 2 weeks, water without or with supplementation of either a -tocopherol, ascorbic acid, riboflavin or a multivitamin preparation. The supplementation period was followed by a 2-week wash-out time interval during which control and multivitamin groups received deionized water. Macrophage stimulation tests using chemiluminescent spectroscopy were performed at the end of the supplementation and wash-out periods to determine cell counts and their capacity to produce free radicals. Multivitamin supplementation increased the number, and the reactive oxygen species-producing activity, of macrophages. This effect persisted for 2 weeks after higher doses of supplementation were stopped. Multivitamin supplementation lowered the steady-state free radical concentrations of liver and spleen as measured by electron paramagnetic resonance spectroscopy. It also increased the antioxidant reactivity of the same organs, while there was no effect on the free radical concentration and antioxidant capacity of the kidney and brain. When taken regularly, low doses of multivitamin supplementation may have a beneficial effect on the defence mechanisms of the organism.Vitamins: Reactive oxygen species: Chemiluminescence: Respiratory burst Nutrition has a great importance in the maintenance of immunity and health of both man and animals (for reviews see:

show abstract

“…Additional, but nontoxic, amounts of retinyl palmitate have been shown to lower the susceptibility of rats to salmonella infection in vivo by enhancing phagocytic functions of isolated Kupffer cells, peritoneal and splenic macrophages (Hatchigian et al 1989). Dietary deprivation of B vitamins, and especially that of riboflavin, significantly lowers the macrophage counts in murine spleen (Matthews et al 1994). Treatment of monocytic human cell lines with cholecalciferol in vitro increases their phagocytic activity and enhances their oxidative burst capacity (Hauck et al 1997).…”

mentioning

confidence: 99%

Effect of multivitamins in an effervescent preparation on the respiratory burst of peritoneal macrophages in mice

Jakus

¹

,

Kriska

²

,

Vanyúr

³

2002

View full text Add to dashboard Cite

The effect of regular intake of low doses of an effervescent multivitamin preparation on the free-radical-producing activity of murine peritoneal macrophages under conditions resembling a possible infection was studied in vitro. Initially, several groups of mice were fed a basal diet and given, for 2 weeks, water without or with supplementation of either a -tocopherol, ascorbic acid, riboflavin or a multivitamin preparation. The supplementation period was followed by a 2-week wash-out time interval during which control and multivitamin groups received deionized water. Macrophage stimulation tests using chemiluminescent spectroscopy were performed at the end of the supplementation and wash-out periods to determine cell counts and their capacity to produce free radicals. Multivitamin supplementation increased the number, and the reactive oxygen species-producing activity, of macrophages. This effect persisted for 2 weeks after higher doses of supplementation were stopped. Multivitamin supplementation lowered the steady-state free radical concentrations of liver and spleen as measured by electron paramagnetic resonance spectroscopy. It also increased the antioxidant reactivity of the same organs, while there was no effect on the free radical concentration and antioxidant capacity of the kidney and brain. When taken regularly, low doses of multivitamin supplementation may have a beneficial effect on the defence mechanisms of the organism.Vitamins: Reactive oxygen species: Chemiluminescence: Respiratory burst Nutrition has a great importance in the maintenance of immunity and health of both man and animals (for reviews see:

show abstract

Evaluation of the Immune System in the Nutritionally At-Risk Host

Powell¹,

Borchers²,

Yoshida³

et al. 2000

Nutrition and Immunology

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Dietary Deprivation of B-Vitamins Reflected in Murine Splenocyte Proliferation in Vitro

Cited by 4 publications

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Effect of multivitamins in an effervescent preparation on the respiratory burst of peritoneal macrophages in mice

Effect of multivitamins in an effervescent preparation on the respiratory burst of peritoneal macrophages in mice

Effect of multivitamins in an effervescent preparation on the respiratory burst of peritoneal macrophages in mice

Evaluation of the Immune System in the Nutritionally At-Risk Host

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