Dietary conjugated linoleic acid supplementation alters the expression of genes involved in the endocannabinoid system in the bovine endometrium and increases plasma progesterone concentrations

Abolghasemi, Armita; Dirandeh, E.; Pirsaraei, Zarbakht Ansari; Shohreh, Bahram

doi:10.1016/j.theriogenology.2016.05.003

Cited by 16 publications

(8 citation statements)

References 38 publications

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Section: Discussionmentioning

confidence: 99%

“…The main components of the endocannabinoid system (CB2 receptor and the enzymes NAPEPLD and FAAH) coexist in various kinds of cells in the human (Taylor, Abbas, Habiba, & Konje, 2010) and bovine endometrium (Abolghasemi et al, 2016). Iuvone et al (2008) have suggested that in women who effected by endometrial inflammation, there is an upregulation of CB-2 receptor.…”

Section: Discussionmentioning

confidence: 99%

“…Samples were expressed relative to ubiquitin as a housekeeping gene, which was stable under the culture conditions used compared to 18Srna and GAPDH. The primers used in this study have been published (Abolghasemi, Dirandeh, Ansari Pirsaraei, & Shohreh, 2016;Salehi, Colazo, Gobikrushanth, Basu, & Ambrose, 2017). Data were normalized to a calibrator sample using the ΔΔCt method.…”

Section: Real-time Pcrmentioning

confidence: 99%

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Endocannabinoids as endometrial inflammatory markers in lactating Holstein cows

Bonsale

Sharifi

Dirandeh

et al. 2018

Reprod Domestic Animals

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The objective of this study was to consider endocannabinoid system as inflammatory markers in bovine endometrium to better understand the role of this system in regulating many of the functions that are related to inflammatory condition. At day 26 post-partum, fourteen cows were divided into two groups depending on the inflammatory condition: 1- subclinical endometritis (n = 7, with purulent or mucopurulent uterine discharge detectable in the vagina) and 2- healthy (n = 7, No (muco)) purulent discharge. Blood samples were collected at 26 and 30 days relative to calving to determine plasma tumour necrosis factor (TNF) and lipopolysaccharide-binding protein (LBP) concentrations; moreover, uterine biopsy was carried out on day 26 post-partum to measure mRNA abundance of TNF, interleukin-1B (IL1B), interleukin-6 (IL-6), C-X-C motif chemokine ligand 8 (CXCL8), endocannabinoid receptor (CNR2), N-acyl phosphatidylethanolamine phospholipase D (NAPEPLD), fatty acid amide hydrolase (FAAH), N-acylethanolamine acid amidase (NAAA) and monoglyceride lipase (MGLL) by real-time PCR. Results showed mean plasma concentrations of TNF and LBP were lower in healthy cows compared to subclinical endometritis cows (p < .05). Relative mRNA expression for NAAA and FAAH was decreased (p < .05), and relative mRNA expression for CNR2 and NAPEPLD increased in cows with subclinical endometritis compared to healthy cows. In conclusion, relative mRNA expression of TNF, IL1B and CXCL8 and plasma concentration of LBP increased during inflammatory condition along with decreased endocannabinoids hydrolyzing enzyme (NAAA and FAAH), increased enzymes that synthesize endocannabinoids (NAPEPLD) and relative gene expression of the endocannabinoid receptor; together, these contribute to increased endocannabinoids levels during inflammation. Overall, we provide evidence that endocannabinoid system is altered in endometrium tissue during inflammation through increased mRNA expression of CNR2 and synthesis enzyme and decreased mRNA expression of hydrolyzing enzymes interfere with pro-cytokine production and signalling, which may interfere with the onset and progression of inflammation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Real-time Pcrmentioning

confidence: 99%

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Endocannabinoids as endometrial inflammatory markers in lactating Holstein cows

Bonsale

Sharifi

Dirandeh

et al. 2018

Reprod Domestic Animals

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“…Dietary CLA has been shown to decrease the levels of the endocannabinoid 2-arachidonoyl-glycerol (2-AG) in the cerebral cortex of mice [55], and rats fed with 1% CLA (mixture of c9,t11 and t10,c12 isomers) had increased levels of the endocannabinoids N-oleoylethanolamide (OEA) and N-palmitoylethanolamide (PEA) in the liver. In dairy cows, dietary CLA decreased the mRNA expression of the CB2 in the uterus [56]. Based on this, we suggest that the lower expression and abundance of CB1 in the AT of the CLA-supplemented cows may be a negative feedback to the lipogenic signals that were induced following CLA supplementation in order to control AT lipogenesis; however, this issue requires further investigation.…”

Section: Discussionmentioning

confidence: 81%

Phosphoproteomic Analysis of Subcutaneous and Omental Adipose Tissue Reveals Increased Lipid Turnover in Dairy Cows Supplemented with Conjugated Linoleic Acid

Daddam

Hammon

Tröscher

et al. 2021

IJMS

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Phosphoproteomics is a cutting-edge technique that can be utilized to explore adipose tissue (AT) metabolism by quantifying the repertoire of phospho-peptides (PP) in AT. Dairy cows were supplemented with conjugated linoleic acid (CLA, n = 5) or a control diet (CON, n = 5) from 63 d prepartum to 63 d postpartum; cows were slaughtered at 63 d postpartum and AT was collected. We performed a quantitative phosphoproteomics analysis of subcutaneous (SC) and omental (OM) AT using nanoUPLC-MS/MS and examined the effects of CLA supplementation on the change in the phosphoproteome. A total of 5919 PP were detected in AT, and the abundance of 854 (14.4%) were differential between CON and CLA AT (p ≤ 0.05 and fold change ± 1.5). The abundance of 470 PP (7.9%) differed between OM and SC AT, and the interaction treatment vs. AT depot was significant for 205 PP (3.5% of total PP). The integrated phosphoproteome demonstrated the up- and downregulation of PP from proteins related to lipolysis and lipogenesis, and phosphorylation events in multiple pathways, including the regulation of lipolysis in adipocytes, mTOR signaling, insulin signaling, AMPK signaling, and glycolysis. The differential regulation of phosphosite on a serine residue (S777) of fatty acid synthase (FASN) in AT of CLA-supplemented cows was related to lipogenesis and with more phosphorylation sites compared to acetyl-coenzyme A synthetase (ACSS2). Increased protein phosphorylation was seen in acetyl-CoA carboxylase 1 (ACACA;8 PP), FASN (9 PP), hormone sensitive lipase (LIPE;6 PP), perilipin (PLIN;3 PP), and diacylglycerol lipase alpha (DAGLA;1 PP) in CLA vs. CON AT. The relative gene expression in the SC and OM AT revealed an increase in LIPE and FASN in CLA compared to CON AT. In addition, the expression of DAGLA, which is a lipid metabolism enzyme related to the endocannabinoid system, was 1.6-fold higher in CLA vs. CON AT, and the expression of the cannabinoid receptor CNR1 was reduced in CLA vs. CON AT. Immunoblots of SC and OM AT showed an increased abundance of FASN and a lower abundance of CB1 in CLA vs. CON. This study presents a complete map of the SC and the OM AT phosphoproteome in dairy cows following CLA supplementation and discloses many unknown phosphorylation sites, suggestive of increased lipid turnover in AT, for further functional investigation.

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“…Veth et al (99) evaluated studies that indicated a reduction in pregnancy time, from 151 to 117 days, when cows were supplemented with rumen-protected CLA. Abolghasemi et al (102) suggest that the CLA-enriched diet has beneficial effects such as reduced expression of the receptor endocannabinoid (CNR2) and enzymes that synthesize fatty acid amides (NAPEPLD), in addition to an increase in PTGS2, resulting in an increase in plasma progesterone measurements during early lactation. Oliveira et al (103) supplemented cows with CLA 18 days before parturition and observed that serum fat and β-hydroxybutyrate were reduced on days 1 and 7 postpartum, resulting in a lower prevalence of hyperketonemia on day 14 postpartum.…”

Section: Effects Of Cla Diet Supplementation On Fertilitymentioning

confidence: 99%

Conjugated linoleic acid as a potential bioactive molecule to modulates gamete and embryo cryotolerance

et al. 2020

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Conjugated linoleic acid (CLA) is a mixture of positional isomers of linoleic acid found in meat and dairy products from ruminants. It is a trans fat widely used by athletes as a food supplement, due to a supposed effect of maximizing the use of body fat reserves. The interest in diet and culture media supplementation with CLA is an emerging area, demanding studies in order to elucidate its benefits in the reproductive parameters, as well as in cryopreservation. Therefore, the aim of this review was to discuss the effects of CLA on the oocytes, sperm and embryos cryotolerance. Some studies have already demonstrated its use in cryopreservation of germline. Among those, it was observed that CLA supplementation during oocyte in vitro maturation can increase their viability post-freezing and developmental capacity. Regarding the use of CLA on sperm, there are few studies and their results are still inconclusive. Finally, studies about CLA supplementation on embryo culture media have shown promising results, indicating that this bioactive molecule is able to modulate lipid uptake on blastomeres. Altogether, these findings demonstrate the potential use of CLA as a bioactive molecule to improve germline and embryo cryotolerance and open new perspectives on human and animal reproduction field.

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Dietary conjugated linoleic acid supplementation alters the expression of genes involved in the endocannabinoid system in the bovine endometrium and increases plasma progesterone concentrations

Cited by 16 publications

References 38 publications

Endocannabinoids as endometrial inflammatory markers in lactating Holstein cows

Endocannabinoids as endometrial inflammatory markers in lactating Holstein cows

Phosphoproteomic Analysis of Subcutaneous and Omental Adipose Tissue Reveals Increased Lipid Turnover in Dairy Cows Supplemented with Conjugated Linoleic Acid

Conjugated linoleic acid as a potential bioactive molecule to modulates gamete and embryo cryotolerance

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