Dietary Conjugated Linoleic Acid Increases Immunoglobulin Productivity of Sprague-Dawley Rat Spleen Lymphocytes

Yamasaki, Masao; Kishihara, Koji; Mansho, Keiko; Ogino, Yoichiro; Kasai, Michio; Sugano, Michihiro; Tachibana, Hirofumi; Yamada, Koji

doi:10.1271/bbb.64.2159

Cited by 58 publications

(39 citation statements)

References 22 publications

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“…This is the first known report of a change in IgE with CLA ingestion. Yamasaki et al (2000) observed that CLA enhanced overall Ig production in rat spleen but did not affect serum IgG levels. These observations are in general agreement with our present findings in human volunteers which showed that plasma IgA was significantly increased (Po0.05) but IgE was decreased (Po0.05) during and after CLA supplementation suggesting a decreased sensitivity to allergen actions.…”

Section: Discussionmentioning

confidence: 82%

Effect of CLA supplementation on immune function in young healthy volunteers

et al. 2005

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Objectives: This study investigated the effect of dietary CLA supplementation (3g/day; 50:50 mix of the two major isomers) on the immune system and plasma lipids and glucose of healthy human (male and female) volunteers. Design: Double-blind, randomized, reference-controlled study. Subject and intervention: A total of 28 healthy male and female participants aged 25-50 y received either high oleic sunflower oil (reference) or 50% CLA 9-11 and 50% CLA 10-12 CLA isomers (50:50 CLA-triglyceride form). The treatments were given as supplements in soft-gel capsules providing a total 3 g (6 Â 500 mg capsules) per day in treatment groups for 12 weeks. A 12-week washout period followed the intervention period. Results: Levels of plasma IgA and IgM were increased (Po0.05 and 0.01 respectively), while plasma IgE levels were decreased (Po0.05). CLA supplementation also decreased the levels of the proinflammatory cytokines, TNF-a and IL-1b (Po0.05), but increased the levels of the anti-inflammatory cytokine, IL-10 (Po0.05). Another aspect of immune function, delayed type hypersensitivity (DTH) response, was decreased during and after CLA supplementation (Po0.05). However, plasma glucose, lipids, lymphocyte phenotypic results were not affected significantly by CLA. Conclusion: This is the first study to show that CLA, a fatty acid naturally found in dairy and meat products, can beneficially affect immune function in healthy human volunteers. Sponsorship: This study was supported by Loders-Croklaan, The Netherlands and SEERAD (Scottish Executive Environmental Rural and Agriculture Department).

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Section: Discussionmentioning

confidence: 82%

Effect of CLA supplementation on immune function in young healthy volunteers

et al. 2005

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“…Song et al (36) reported a similar effect in humans after supplementation with a 50:50 CLA isomer mixture for 12 wk. However, Yamasaki et al (37) found no significant effects on serum IgA, IgG, or IgM concentration after feeding 5-wk-old rats a 50:50 CLA isomer mixture for 3 wk at doses ranging from 0.05% to 0.5% (38). Other studies have additionally suggested that the effects of CLA intake during developmental phases are manifested beyond the supplementation period (30).…”

Section: Discussionmentioning

confidence: 99%

Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy

Pérez-Cano

Ramírez-Santana

Molero-Luís

et al. 2009

Journal of Lipid Research

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The aim of this work was to establish the effect of the cis9,trans11 conjugated linoleic acid (CLA) isomer on mucosal immunity during early life in rats, a period when mucosal immunoglobulin production is poorly developed, as is also the case in humans. CLA supplementation was performed during three life periods: gestation, suckling, and early infancy. The immune status of supplemented animals was evaluated at two time points: at the end of the suckling period (21-day-old rats) and 1 week after weaning (28-dayold rats). Secretory IgA was quantified in intestinal washes from 28-day-old rats by ELISA technique. IgA, TGFb, and PPARg mRNA expression was measured in small intestine and colon by real time PCR, using Taqman: specific probes and primers. IgA mucosal production was enhanced in animals supplemented with CLA during suckling and early infancy: in 28-day-old rats, IgA mRNA expression was increased in small intestine and colon by approximately 6-and 4-fold, respectively, and intestinal IgA protein by ?2-fold. TGFb gene expression was independent of age and type of tissue considered, and was not modified by dietary CLA. Gene expression of PPARg, a possible mediator of CLAʼs effects was also upregulated in animals receiving CLA during early life. In conclusion, dietary supplementation with CLA during suckling and extended to early infancy enhances development of the intestinal immune response in rats.-Pérez-Cano, F. J., C. Ramírez-Santana, M. Molero-Luís, M. Castell, M. Rivero, C. Castellote, and À. Franch. Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy.

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“…However, no effect on lymphoproliferation is observed in neonatal animals fed CLA [21]. On the other hand, although several studies in adult animals following CLA diets do not modify in vitro or in vivo immunoglobulin (Ig) production [19,20,22,23], some enhancing effects on immunoglobulin synthesis are described for neonatal and young rodents after CLA consumption [21,24,25].…”

Section: Introductionmentioning

confidence: 99%