Dietary carbohydrate level affects transcription factor expression that regulates skeletal muscle myogenesis in rainbow trout

Chapalamadugu, Kalyan C.; Robison, Barrie D.; Drew, Robert E.; Powell, Madison S.; Hill, Rodney A.; Amberg, Jon J.; Rodnick, Kenneth J.; Hardy, Ronald W.; Hill, M.; Murdoch, Gordon K.

doi:10.1016/j.cbpb.2009.01.013

Cited by 42 publications

(35 citation statements)

References 45 publications

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“…67 The lack of effect of dietary carbohydrate-to-protein ratio in the expression of myogenic regulatory factors coding genes contradicts also with a recent study in rainbow trout, showing that carbohydrate levels in the diet affect the expression of Myf5 and myogenin. 25 However, these results were obtained after 12 weeks of feeding, possibly reflecting that the effect of manipulating dietary macronutrient composition on the expression of these genes appears at long-term but not during the postprandial period. Furthermore, in contrast to zebrafish, rainbow trout displays an indeterminate muscle growth pattern allowed by a continuous production of muscle fibers (hyperplasia) in addition to an increase of fiber size (hypertrophy).…”

Section: Myogenesismentioning

confidence: 46%

“…Elevated glucose concentrations have thus been associated with an increase of the expression of several genes coding for enzymes needed for de novo lipogenesis such as acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), and liver pyruvate kinase (L-PK), [21][22][23][24] but also that of several myogenic regulatory factors coding genes. 25 In recent years, great progress has been achieved in understanding the molecular mechanisms that couple glucose availability to gene transcription. 20 However, these mechanisms have not been fully characterized to date and remain an important field of research for study of the regulation of physiological functions of individuals and/or animals living under conditions of restricted, imbalanced, or excessive food intake.…”

Section: Introductionmentioning

confidence: 99%

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Postprandial Regulation of Growth- and Metabolism-Related Factors in Zebrafish

et al. 2013

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Zebrafish (Danio rerio) have been proposed as a possible model organism for nutritional physiology. However, this potential has not yet been realized and studies on the field remain scarce. In this work, we investigated in this species the effect of a single meal as well as that of an increase in the ratio of dietary carbohydrates/proteins on the postprandial expression of several hepatic and muscle metabolism-related genes and proteins. Fish were fed once either a commercial diet (experiment 1) or one of two experimental diets (experiment 2) containing different protein and carbohydrate levels after 72 h of starvation. Refeeding induced the postprandial expression of genes of glycolysis (GK, HK1) and lipogenesis (FAS, G6PDH, ACCa) and inhibited those of gluconeogenesis (cPEPCK) and beta-oxidation (CPT1b) in the viscera. In the muscle, refeeding increased transcript levels of myogenesis (Myf5, Myogenin), inhibited those of Ub-proteasomal proteolytic system (Atrogin1, Murf1a, Murf1b), and induced the activation of key signaling factors of protein synthesis (Akt, 4EBP1, S6K1, S6). However, diet composition had a low impact on the studied factors. Together, these results highlight some specificity of the zebrafish metabolism and demonstrate the interest and the limits of this species as a model organism for nutritional physiology studies.

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Section: Myogenesismentioning

confidence: 46%

Section: Introductionmentioning

confidence: 99%

Postprandial Regulation of Growth- and Metabolism-Related Factors in Zebrafish

et al. 2013

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“…Nevertheless, there has been little research on how fish diets affect the expression of genes that participate in growth. Chapalamadugu et al (2009) evaluated the influence of dietary carbohydrate level (0, 15, 25, or 35%) on the temporal mRNA expression patterns (4, 8 or 12 week) of transcription factors that regulate satellite cell myocyte addition (MA) in rainbow trout. They showed that 15% and 25% carbohydrate containing diets significantly up-regulate Myod and Myf5, but not Pax7 after 12 weeks of feeding.…”

Section: Zebrafish Nutritional Genomicsmentioning

confidence: 99%

Zebrafish as a model organism for nutrition and growth: towards comparative studies of nutritional genomics applied to aquacultured fishes

Ulloa

Iturra

Neira

et al. 2011

Rev Fish Biol Fisheries

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Zebrafish (Danio rerio) is a common research model in fish studies of toxicology, developmental biology, neurobiology and molecular genetics; it has been proposed as a possible model organism for nutrition and growth studies in fish. The advantages of working with zebrafish in these areas are their small size, short generation time (12-14 weeks) and their capacity to produce numerous eggs (100-200 eggs/clutch). Since a wide variety of molecular tools and information are available for genomic analysis, zebrafish has also been proposed as a model for nutritional genomic studies in fish. The detailed study of every species employed as a model organism is important because these species are used to generalize how several biological processes occur in related organisms, and contribute considerably toward improving our understanding of the mechanisms involved in nutrition and growth. The objective of this review is to show the relevant aspects of the nutrition and growth in zebrafish that support its utility as a model organism for nutritional genomics studies. We made a particular emphasis that gene expression and genetic variants in response to zebrafish nutrition will shed light on similar processes in aquacultured fish.

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“…However, significantly greater expression of pax7 in treatments A, B and C (P = 0.014, P = 0.023 and P = 0.033, respectively) compared to control after 36 h of treatment was observed. We detected significantly (P = 0.022) higher expression of pax7 in cells under treatment A during 36 h compared to cells exposed to same treatment during 24 h. We detected no differences in pax7 expression in cells exposed to either treatment B or C during 24 h compared to cells under same treatments during 36 h. The ratio of pax7/myoD was analyzed since it has been previously suggested as a parameter likely to indicate satellite cell fate (Chapalamadugu, et al, 2009;Olguin et al, 2007). The pax7/myoD ratio was significantly (P = 0.021) higher in the control group compared to cells under treatment A during 24 h (Fig.…”

Section: Effect Of Anthocyanidins In Myogenic Programming In Early-inmentioning

confidence: 81%

“…The significant decrease in pax7/myoD mRNA ratio observed in cells exposed to the lower anthocyanidin doses during 24 h compared to control group further reinforces this statement. The pax7/myoD ratio is a determinant factor in myogenic cell fate where a low ratio indicates myogenic cells progress toward terminal differentiation (Chapalamadugu et al, 2009;Olguín et al, 2004, Olguín et al, 2007Olguín and Pisconti, 2012). In the present study an exposure to either anthocyanidin concentrations during 36 h induced an up-regulation of pax7 expression as well as a trend toward higher myoD levels, thus causing an intermediate pax7/myoD ratio in early-induced cells.…”

Section: Discussionmentioning

confidence: 94%