Dietary bioflavonoid quercetin modulates porcine ovarian granulosa cell functions <i>in vitro</i>

Kolesárová, Adriana; Roychoudhury, Shubhadeep; Klinerova, Bibiana; Packová, Dagmara; Michalcová, Katarína; Halenár, Marek; Kopčeková, Jana; Mňahončáková, Erika; Gálik, Branislav

doi:10.1080/03601234.2019.1586034

Cited by 21 publications

(13 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study thus represents the first assessment of quercetin effects on human oocytes. Moreover, our demonstration of multiple quercetin-related improvements in oocytes from aged mice corroborates findings from studies in swine 24,38 . The present study fundamentally supports further exploration of the use of quercetin to improve the IVM of oocytes derived from aging patients suffering from reproductive dysfunction.…”

Section: Discussionsupporting

confidence: 86%

Quercetin promotes in vitro maturation of oocytes from humans and aged mice

et al. 2020

View full text Add to dashboard Cite

Maternal fertility declines irreversibly with aging, and advanced maternal age is mostly related to impaired oocyte quality. The flavonol compound quercetin is considered to be an anti-aging agent due to its cytoprotective actions as an antioxidant. However, its role and mechanisms on aged oocytes are unclear. In this study, the quercetin promotes in vitro maturation (IVM) and early embryonic development of oocytes from aged mice. It is extended these findings in human oocytes, showing that quercetin promotes the IVM rate by 19.6% and increases the blastocyst formation rate by 15.5% compared to untreated controls. The overall oocyte quality of aged mice is improved by quercetin treatment, assessed as spindle/chromosome morphology and cortical granule distribution. Mitochondria is the primary endogenous source of age-related oxidative stress, and an RNA-seq analysis of quercetin-treated oocytes reveals molecular insights including scavenged mitochondrial-ROS, reduced apoptosis, and improved autophagy. Further, this study demonstrates that quercetin reduces ROS via SIRT3-mediated acetylation of SOD2’s K68 residue. Thus, beyond demonstrating that quercetin confers beneficial mitochondria-related impacts in aged oocytes, this study illustrates a potential strategy to prevent or delay oocyte aging and to improve success rates of assisted human reproductive technologies (ART).

show abstract

Section: Discussionsupporting

confidence: 86%

Quercetin promotes in vitro maturation of oocytes from humans and aged mice

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The current results that Que increased secretion of P 4 and expressions of steroidogenesis‐related genes (StAR, CYP11A1) were similar to previous reports that low doses of flavonoids improved secretion of P 4 by stimulating the expression of StAR, CYP11A1 , and FDX1 in Leydig cells, swine GCs, and laying hens. [ 51–54 ] However, other studies showed that 50‐µg/mL Que had no effects on the viability of swine GCs, but it inhibited the secretion of P 4 in a dose‐related manner (0‐50 µg/mL) due to the suppression of steroidogenic enzymes. [ 55 ] These differences were probably due to the fact that our experiments were conducted on goat LGCs rather than GCs or other cell models.…”

Section: Discussionmentioning

confidence: 99%

Quercetin alleviated H₂O₂‐induced apoptosis and steroidogenic impairment in goat luteinized granulosa cells

Yang

et al. 2020

J Biochem & Molecular Tox

View full text Add to dashboard Cite

Quercetin (Que) is a natural flavonoid in most plants. Luteinized granulosa cell (LGC) culture is necessary for the study of follicle growth/differentiation. In the present study, we analyzed the role of Que in steroid production and apoptosis in hydrogen peroxide (H2O2)‐treated goat LGCs. The results showed that treatment with H2O2 induced apoptosis in goat LGCs, and treatment with Que decreased LGC apoptosis induced by H2O2 (P < .05), accompanied with the different expressions of BAX, BCL‐2, Caspase 3, and Cleaved caspase 3. Meanwhile, the messenger RNA expressions of nuclear factor erythroid 2 like 2 (Nrf2) and its downstream genes were upregulated with H2O2 +Que treatment, accompanied by the increased cellular viability (P < .05). Furthermore, Que alleviated H2O2‐induced reduction in the secretion of progesterone (P4) (P < .05); however, it had no effect on the secretion of estrogen (E2). Simultaneously, the expressions of StAR and P450scc were increased when treated with Que +H2O2, compared with the group treated with only H2O2 (P < .05). In conclusion, it is observed that Que could alleviate the H2O2‐induced apoptosis and steroidogenic impairment in goat LGCs, which might be mediated by the Nrf2 pathway.

show abstract

“…An added advantage of the therapeutic use of quercetin is the lack of negative influences on the function of normal ovarian cells. Kolesarova et al [28] validated its safety in porcine ovarian granulosa cells and also reported that quercetin was able to promote the release of progesterone and inhibit p53 at 10μmol L -1 concentration, which suggests its dose-dependent effect on the function of ovarian cells. Contrary to these findings, Tarko and colleagues [29] reported that quercetin inhibited the growth of normal porcine ovarian granulosa cells at concentrations higher than 10μg mL -1 and promoted apoptosis, whereas at concentrations below 10μg mL -1 no effect on cell proliferation could be observed.…”

Section: Quercetin Mediated Cell Death In Ovarian Cancermentioning

confidence: 94%

Quercetin and MicroRNA Interplay in Apoptosis Regulation in Ovarian Cancer

Khan

Javed

Sadia

et al. 2021

CPD

View full text Add to dashboard Cite

: The multifaceted nature of ovarian cancer has severely hampered the development of effective therapeutics over the years. The composite nature of ovarian cancer makes it therapeutically challenging, therefore, there has been a renewed interest in phytochemistry. Phytochemicals have emerged as a potential therapeutic option due to their limited cytotoxicity and high specificity. Moreover, their signaling inhibition properties have also been studied extensively in recent times. A growing number of data obtained through high-throughput technologies has started to delineate the complex oncogenic signaling networks, thus broadening the therapeutic opportunities. Within such network, microRNAs (miRNAs) have been shown to play a versatile role in the regulation of cancer. Quercetin has been in the spotlight over the years because of its high pharmacological values and substantial evidence has demonstrated its anti-proliferative efficacy against various types of cancers. Despite the versatility of quercetin, little is known about its anti-proliferative potential towards ovarian cancer. This review sheds some light on the importance of quercetin as an alternative therapeutic approach to this tumor, furthermore addressing the interplay between miRNAs and quercetin in the regulation of apoptosis in ovarian cancer.

show abstract

Dietary bioflavonoid quercetin modulates porcine ovarian granulosa cell functions in vitro

Cited by 21 publications

References 25 publications

Quercetin promotes in vitro maturation of oocytes from humans and aged mice

Quercetin promotes in vitro maturation of oocytes from humans and aged mice

Quercetin alleviated H₂O₂‐induced apoptosis and steroidogenic impairment in goat luteinized granulosa cells

Quercetin and MicroRNA Interplay in Apoptosis Regulation in Ovarian Cancer

Contact Info

Product

Resources

About

Dietary bioflavonoid quercetin modulates porcine ovarian granulosa cell functions in vitro

Cited by 21 publications

References 25 publications

Quercetin promotes in vitro maturation of oocytes from humans and aged mice

Quercetin promotes in vitro maturation of oocytes from humans and aged mice

Quercetin alleviated H2O2‐induced apoptosis and steroidogenic impairment in goat luteinized granulosa cells

Quercetin and MicroRNA Interplay in Apoptosis Regulation in Ovarian Cancer

Contact Info

Product

Resources

About

Quercetin alleviated H₂O₂‐induced apoptosis and steroidogenic impairment in goat luteinized granulosa cells